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Biomedical subjects

M Mazurkiewicz

Publications and source records attributed to M Mazurkiewicz.

At least 19 recordsLinked to original sources

The clinical course of the Infectious Laryngotracheitis (ILT) field case in hens and estimation of immunoprophylaxis efficiency.

The ILT case was observed in laying hen farm where birds of different age (from 40 to 107 weeks) were kept in 10 flocks. A rapid spread of the disease, the decrease in egg production (in flock No. 1 it reached 58%) and higher mortality (the highest in 76 and 77 week-old birds, accounting for 0.11% and 0.36%, respectively ) was recorded during first 2 weeks of disease. Antibodies against ILT virus were detected in serum of the examined birds during the whole observation period (50 weeks after the disease outbreak). The laying hens were vaccinated at 8 weeks of age and boosted after 5 weeks. The vaccine was applied in drinking water, in a dose twice as high as usually recommended per one bird. Immunopropylaxis efficiency was estimated on the basis of immunological response in birds (serum samples, ILT ELISA kit, Guildhay Ltd.) and general health status of hens in flocks. Postvaccinal immunity, the presence of specific antibodies against ILT, was observed in all birds during the observation period (51 weeks). During that time GMT value ranged from 8261,3 (week 10) to 5196 (week 51) after the second vaccination, and CV amounted in this period to 41.1% and 51%, respectively. Subsequently, clinical symptoms of the disease disappeared and the egg production, as well as mortality, returned to the level of technological norms for laying hens.

Animals↗

A multicentre randomized/controlled trial of a conventional versus modestly accelerated radiotherapy in the laryngeal cancer: influence of a 1 week shortening overall time.

BACKGROUND AND PURPOSE: To compare in a phase III study the loco-regional control, disease-free survival and overall survival induced by an accelerated regimen (AF) as compared with conventional regimen (CF) and to analyze the early and late post-radiation morbidity in both arms. MATERIALS AND METHODS: Patients with age < or = 75, WHO 0-1, suitable for a radical course of radiotherapy T1-T3, N0, M0, stage of glottic and supraglottic laryngeal cancer were randomized to either CF: 66Gy given in 33 fractions over 45 days or AF: 66Gy given in 33 fractions over 38 days (2 fractions every Thursday). A total of 395 patients were included from 05.1995 to 12.1998. RESULTS: Early toxicity: At the end of radiotherapy patients treated with AF complained for more severe reactions than patients treated with CF. In 8 weeks after treatment completion patients treated with AF complained only for more severe pain on swallowing (P=0.027). In 4 months after treatment completion all types of toxicity except for skin teleangiectasia (P=0.001) were similar in the two groups. Loco-regional control: comparison between CF and AF showed no difference in terms of loco-regional control (P=0.37). CONCLUSIONS: The improvement in AF in terms of loco-regional control is estimated to be 3-5% in comparison with conventional regimen and is not significant. The intensity of reactions after 4 months was similar in both arms, what suggests the possibility of further shortening of the overall time by few days or enhancing the total dose within the limits of acceptable morbidity.

Aged↗

Comparison of ITS profiling, REP- and ERIC-PCR of Salmonella Enteritidis isolates from Poland.

Thirty-one Salmonella Enteritidis strains isolated from chickens, broilers and hens were analysed by genotypic typing including REP-PCR. ERIC-PCR and ITS profiling (PCR-ribotyping). Analysis of DNA banding patterns generated by REP-PCR revealed the presence of 22 different genotypes, which were grouped by dendrogram analysis into three distinct lineages (maximum similarity approx. 50%). Each isolate of S. Enteritidis analysed by ERIC-PCR generated an individual DNA pattern. Again, these isolates could be divided into three distinct genomic groups (maximum similarity approx. 60%) by their ERIC-PCR fingerprints. REP- and ERIC-PCR were found to be more discriminatory for typing of S. Enteritidis than ITS profiling. Amplification of the 16S-23S rDNA spacer region gave nine different profiles of DNA, subdivided into two closely related groups by dendrogram analysis. In summary, data obtained by genotyping methods for S. Enteritidis isolates from regions located in the south-west and the central parts of Poland revealed an enormous heterogeneity among analysed samples, and proved that REP- and ERIC-PCR are highly discriminatory techniques, which can be used, in addition to conventional methods, in epidemiological studies of S. Enteritidis infections.

Animals↗

[Cisplatin and vinorelbine therapy of previously treated advanced breast cancer (preliminary studies)].

In this work it was investigated the efficacy of second and third line chemotherapy (vinorelbine, cisplatin/carboplatin) in patients with metastases breast cancer. The twenty pre-treated women with metastases to the liver, lung, lymph nodules and subcutaneous tissue were treated the regiment including navelbine 30 mg day 1 and 5 and cisplatin 100 mg/m2 day 1 every 3 weeks. The patients had been early received CMF and/or FAC. The total response rate was 40%. The median time to progression was 8 months. The stabilisation disease was at 20% patients. The tolerance of treatment was good and the frequent complication was leucopenia (WHO 2 and 3) and thrombocytopenia (WHO 1). The women received from 6 to 10 courses.

Adult↗

Intercellular cytoplasm transport during oogenesis of the moth midge, Tinearia alternata Say (Diptera: Psychodidae).

Polytrophic ovaries of the nematocerous dipteran, Tinearia alternata Say consists of several developmentally synchronized ovarioles each housing only one functional egg chamber with 15 nurse cells and an oocyte. At the early stages of previtellogenesis the nurse cells become polyploid and synthetically active. Their nuclei contain polytene chromosomes and prominent nucleoli. With the advance of previtellogenic growth the nurse cell cytoplasm is loaded with the growing number of ribosomes and contain perinuclear nuage material, mitochondria, electron dense bodies and aggregations of endoplasmic reticulum. All these organelles are transported into the oocyte thanks to the massive and rapid flow of the nurse cell cytoplasmic contents. Nurse cell-oocyte transport is mediated by actin cytoskeleton. Prior to the rapid cytoplasm transfer, F-actin network is associated with the nurse cell membranes while tiny bundles of microfilaments form actin baskets connected with ring canals. Nurse cells in Tinearia lack an extensive scaffold of radially oriented, F-actin bundles (cables) that would tether their nuclei in place, thus preventing ring canals from plugging. The way the nuclei are anchored to their central positions within the cells remains unclear. Towards the final stages of oogenesis nurse cells are almost devoid of cytoplasm and degenerate. Although their nuclei undergo dramatic morphological transformations, typical hallmarks of apoptotic pathway could not be clearly observed. Rapid ooplasmic streaming does not occur.

Actin Cytoskeleton↗

[Induction chemotherapy and radiotherapy in locally advanced head and neck carcinoma].

The value of inductive chemotherapy preceding the radical irradiation of locally advanced neoplasms of head and neck has been estimated. The following have been applied: cisplatin, adriamycin, bleomycin, or cyclophosphamide, adriamycin, 5-fluorouracil, or cisplatin 5-fluorouracil. A reaction to treatment in the form of CR and PR has been achieved in 71.5% of the treated patients. Irradiation was started in two to four weeks after chemotherapy had been stopped. After combined treatment the proportion of 69.5% CR and PR was achieved. The average length of time of CR and PR was 8.7 months. Treatment was well tolerated.

Adult↗

[Operations of parotid malignant tumors].

In a group of 64 patients with malignant tumours of the parotid gland the most common type was adenoid cystic carcinoma (20%) and malignant pleomorphic adenoma (14%). A group of 33 patients with follow-up from 4 months to 18 years was analyzed. The overall 5-year survival rate was 45%.

Adolescent↗

The role of GABA-ergic system in human mammary gland pathology and in growth of transplantable murine mammary cancer.

In this paper we described the results of our studies on the baclophen (gamma aminobutyric acid (GABA)-B receptor agonist) inhibitory effect on the growth of experimental mammary cancer 16/C in mice and on the estimation of GABA level and GAD (glutamine acid decarboxylase--the key enzyme in GABA synthesis) activity after this treatment in mice. The experimental data are confronted with the estimation of GABA level and GAD activity in human mammary gland material taken from the patients with benign breast tumors of different pathological and age related hormonal stages. A significant inhibition of 16/C tumor growth in treated with baclophen mice was observed. Mean GABA level and GAD activity were significantly higher both in tumor and in normal tissue of baclophen treated mice in comparison to control animals. The results of clinical studies have shown that the lowest GABA level and GAD activity in tumor and normal mammary gland tissue was detected in patients in peri-menopausal stage. Both, in human and mouse material, the GABA level and GAD activity were higher in tumor than in normal tissue and there was a clear positive correlation between GABA level and GAD activity in both tissues studied. GABA level and GAD activity in tumor and in normal tissue were lower in patients with dysplasia than in patients with fibroadenoma. Considering our results, namely an inhibitory effect of GABA receptor agonist on mammary cancer growth and the correlation between GABA level and the stage of breast pathology and/or hormonal activity, it seems probable that GABA-ergic system is involved in hormonal regulation and pathogenesis of breast cancer.

Adolescent↗

GABA level and GAD activity in human and mouse normal and neoplastic mammary gland.

Our studies have demonstrated that GABA (gamma-aminobutyric acid) is detectable both in mouse and in human normal mammary gland and in neoplastic alterations. We have also shown that GABA content in tumor was significantly higher than in normal tissue. The statistically significant difference in GAD (glutamine acid decarboxylase) activity between tumor and normal mammary tissue was also detected. The positive correlation between GABA content and GAD activity in tumor cells was observed both in human and in mice materials. The observed increase in GABA level and GAD activity in tumor tissue could reflect an eventual local antitumor immune response, however, a hypoxia of tumor cells could also be considered. The role of GABA, GAD and GABA-ergic receptors in cancerogenesis and in cancer progression is still to be clarified and requires further studies; however, it may indicate that the known agonists of GABA-ergic system (e.g. baclofen) can potentially modulate the tumor growth.

Adenocarcinoma↗

Diptera--ovary structure and oogenesis in midges and flies.

Comparative study of ovary development and oogenesis in the dipterans revealed significant differences between the Nematocera (lower dipterans, midges) and the Brachycera (true flies). The occurrence of these differences emphasizes well the phylogenetic division of the Diptera into these major subgroups. Basic discrepancies were found in the course of ovary development and in the mode of follicular cell differentiation. In contrast to more advanced flies, in midges the initial stages of germ cell differentiation, i.e. divisions of gonial cells, germ cell cluster formation and diversification of cystocytes within clusters take place exclusively in the larval and early pupal stages. Moreover, the formation of cystocyte clusters precedes that of ovarioles. Differences in the behaviour of some follicular cells found between the ovarian follicles of midges and advanced flies suggest that both major dipteran subgroups may differ in the scenario and/or the mechanisms of terminal signalling leading to the determination of the anteriormost part of the body.

Animals↗

[The investigation of causes of treatment failures of osteosarcoma].

A series of 57 patients (32 females, 25 males) aged 12-41 (mean 17.2) treated due to IIB advanced osteosarcoma has been analyzed. From among 20 patients who underwent surgery only, 2 persons survived. In 10 patients supplementary chemotherapy or preventive lung radiotherapy has been used. Two patients survived more than 5 years. In past 11 years 4 pre and postoperative chemotherapy protocols have been used. Big number of failures has been noted in the first few years. Despite chemotherapy recurrence took place in 6 cases. Failures resulted also from the late diagnosis or discontinued treatment. During last 5 years all 6 osteosarcoma patients received chemotherapy at Hematology and Pediatric Oncology Department and tumor resection has been performed. All patients stay alive (2 to 5 years).

Adolescent↗

[Acute radiation reaction and its healing time after skin cancer radiotherapy in elderly patients].

In group of 106 patients over 70 with skin cancer radiation-induced reaction and its healing time were evaluated. Mean age was 78.3 years. The control group consisted of 35 patients under 70 with average age 59 years. All patients were treated under conventional roentgenotherapy conditions with dose fractionation. Intensification of acute radioreaction was classified according to RTGO and EORTC scores. In elderly patients the first grade of reaction was twice lower than in control group. Average healing time of radiation-induced reaction in the whole elderly group was 3 weeks longer in comparison with controls. Mean healing time of the same reaction intensity grade was also longer in elderly. In the first grade of changes it was 1 week longer. 3 and 1 week there were in second and third degree respectively.

Acute Disease↗

[Comparison of two irradiation methods for laryngeal carcinoma in the second degree of clinical progression].

Two methods of irradiation of larynx carcinoma have been compared: conventional dose fractionation and hyperfractionation. The first consisted in irradiating once a day a dose of 2.2 GY to the complete dose of 70 Gy to 77 Gy, and the other in applying two fractions daily, 1.1 Gy each, until the combined dose of 70 Gy to 77 Gy was reached. 20 patients irradiated conventionally and 32 treated with hyperfractionation have been examined. The proportion of complete remissions in the compared groups was 65% and 72% respectively. A better tolerance of irradiation by means of hyperfractionation has been recorded. The proportion of I degree and II degree reactions in that group of patients was 75% whereas it was 65% in the group irradiated conventionally.

Adult↗

[The value of inductive chemotherapy in radiation treatment of advanced neoplasms of the head and neck].

The value of inductive chemotherapy preceding the radical irradiation of locally advanced neoplasms of the head and neck has been estimated. The following have been applied: cisplatin, adriamycin, bleomycin. A reaction to treatment in the form of CR and PR has been achieved in 80% of the treated patients. Irradiation was started in two to four weeks after chemotherapy had been stopped. After combined treatment the proportion of 33.3% CR was achieved. The average length of time of CR and PR was 9.8 months.

Adult↗

The effects of selective alpha-2 adrenergic agents on the performance of rats in a 5-choice serial reaction time task.

The present study investigated how the systemic administration of alpha-2 adrenergic agents that modulate the function of the noradrenergic system in brain, affect rousal and sustained attention. Food-deprivated rats were trained to detect and respond to brief flashes of light presented randomly in one of five spatially diverse locations. The effects of single-dose administrations of dexmedetomidine, an alpha-2 agonist, and atipamezole, an alpha-2 antagonists, on the choice accuracy, errors of omissions, speed of responding, and collection of the reward could be assessed in this task. Dexmedetomidine increased the amount of omissions, speed of response, and decreased the number of premature responses, although it did not markedly lengthen response latencies and food collection latency. Atipamezole increased the number of premature responses. Neither dexmedetomidine nor atipamezole had any effect on choice accuracy of rats in this task. The results suggest that dexmedetomidine decreased behavioral activity and arousal of rats, whereas atipamezole had mild stimulant effect on behavior.

Adrenergic alpha-2 Receptor Agonists↗

Dose- and parameter-dependent effects of atipamezole, an alpha 2-antagonist, on the performance of rats in a five-choice serial reaction time task.

The present study investigated whether atipamezole (ATI), a potent alpha 2-adrenoceptor antagonist that increases the release of noradrenaline in brain, improves attention in rats. Thus, the effects of ATI on the performance of adult male rats in the five-choice serial reaction time task were studied. Food-deprived rats were trained to detect and respond to brief flashes of light presented randomly in one of five spatially diverse locations. The effects of single-dose administration of ATI (0.03-3.0 mg/kg) on the performance of rats under different parametric manipulations of the task were tested: 1) the visual stimuli were presented at unpredictable intertrial intervals (ITIs) or b) the intensity (brightness) of visual stimuli was reduced, thus placing an additional load on attentional processing for animals. Presenting the stimuli earlier than normally or reducing its intensity markedly impaired the choice accuracy of rats. At doses of 0.03, 0.3, and 1.0 mg/kg, ATI improved the choice accuracy of rats when tested using reduced stimulus intensity. ATI 3.0 mg/kg did not affect accuracy performance when tested using reduced stimulus intensity but impaired it when tested using unpredictable ITIs. The other doses of ATI (0.03, 0.3, and 1.0 mg/kg) did not markedly affect choice accuracy of rats tested using unpredictable ITI. Our results could be explained by the assumption that an acute, systemic administration of ATI affects arousal mechanisms and facilitates the processing of visual stimuli related to reward.

Adrenergic alpha-Antagonists↗

Effects of an inhibitor of GABA-aminotransferase (gamma-vinyl-GABA) on the spatial navigation deficit induced by nicotinic blockade.

1. The present study investigated whether stimulation of the GABA-ergic system affects spatial navigation (water-maze, WM) deficit induced by nicotinic blockade (mecamylamine). 2. The effects of various doses of gamma-vinyl-GABA (GVG: 50, 150 and 300 mg/kg) and mecamylamine (2.5 and 10 mg/kg) were examined alone and in combination. 3. GVG at the dose 150 mg/kg alone did not impair the performance of rats in the WM task. 4. Mecamylamine at the dose 2.5 and 10 mg/kg clearly impaired the performance of rats in WM task. 5. When the two drugs were co-administered, no interaction between mecamylamine and GVG was observed. 6. Combined nicotinic and muscarinic blockade did not interact as well with GVG administration. 7. Our results do not provide support for any interaction between cholinergic and GABA-ergic mechanisms.

4-Aminobutyrate Transaminase↗

The behavioral effects of serotonin synthesis inhibition and quisqualic acid induced lesions of the nucleus basalis magnocellularis in rats.

1. To investigate the role of the cholinergic and serotonergic systems in the regulation of cognitive functions, the effects of concurrent lesioning of nucleus basalis magnocellularis (NB) with quisqualic acid (quis) and inhibition of brain serotonin synthesis by systemic p-chlorophenylalanine (PCPA) treatment on passive avoidance (PA) retention and water maze (WM) spatial navigation performance were studied in rats. 2. Quis NB lesioning induced a marked reduction (-62%) in frontal cortical choline-acetyltransferase activity, impaired retention of PA, and slightly and transiently impaired acquisition of WM spatial navigation. 3. PCPA (400 mg/kg/day x 3, i.p.) treatment depleted frontal cortical concentrations of both serotonin (82% depletion) and its major metabolite 5-HIAA (90% depletion) and slightly affected the noradrenergic and dopaminergic systems. PCPA treatment alone had no effect on WM or PA behavior, but potentiated the PA retention deficit and slightly aggravated the WM deficit in rats subjected to quis NB lesioning. 4. The present results further support the view that serotonergic and NB neurons interact in the regulation of cognitive functions.

Animals↗