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Biomedical subjects

M Mazza

Publications and source records attributed to M Mazza.

At least 37 records · Page 2Linked to original sources
PubMed1996

Foot and mouth disease virus concentration and purification by affinity chromatography.

Foot and mouth disease virus, (FMDV) from a crude cell lysate was purified in a single step by affinity chromatography with heparin as a ligand. The virus eluted from an Heparin-Ultrogel A4R column at 1M sodium chloride in 10 mM sodium phosphate buffer, pH 7.0, while most cell protein and albumin did so at lower concentrations of sodium chloride in the same buffer. Purity of the eluted fraction containing the virus was assessed by SDS-PAGE, HPLC, ultracentrifugation, and UV absorption spectrum. With this method, intact viral particles are recovered in high yield (over 90%) and specific virus purity increases nearly 1000-fold. The capacity of the chromatographic matrix for the virus was found to be 1.1 mg viral mass per mL of hydrated gel.

Aphthovirus↗
PubMed1996

Putative odorant-binding protein in antennae and legs of Carausius morosus (Insecta, Phasmatodea).

A 19 kDa protein has been purified by gel filtration and anion-exchange chromatography from the antennae of Carausius morosus. Its amino terminal amino acid sequence shows significant similarity (30% identity) with another putative odorant-binding protein, the so called OS-D protein isolated from the antennae of Drosophila melanogaster; only 20% of its amino acids are shared with some members of Lepidoptera pheromone-binding proteins. Polyclonal antibodies, raised against a synthetic amino terminal peptide cross-react with 19 kDa band in the legs extracts, but not with soluble proteins from other parts of the body. The amino terminal sequence of this protein, purified from the legs was identical with that of the antennal protein.

Amino Acid Sequence↗
PubMed1995

Comparison between the effects of neostigmine and ranitidine on interdigestive gastroduodenal motility of patients with gastroparesis.

The antroduodenal motor effects of ranitidine, and H2-receptor antagonist with cholinergic activity, and neostigmine, a cholinomimetic drug, were compared in 16 patients with idiopathic gastroparesis characterized by dysmotility-like dyspepsia, delayed gastric emptying at scintigraphy and absence of gastroduodenal phase 3 of the migrating motor complex during a manometric recording of at least 300 min. After overnight fasting in 8 of these patients, neostigmine was administered intravenously at a dose of 0.5 mg, and in the remaining 8 patients ranitidine was given intravenously at a dose of 100 mg. Ranitidine induced a gastroduodenal phase 3 activity in a significantly (p < 0.02) higher percentage of patients (87.5%) in comparison with neostigmine (25%). In the majority of patients, neostigmine induced only an irregular increase in gastroduodenal motor activity sometimes characterized by propagated and nonpropagated clustered contractions. This property of ranitidine of inducing a phase 3 activity in these patients cannot be ascribed to its weak cholinergic activity, as neostigmine, which has a more intense cholinergic activity than ranitidine, is unable to produce the effect demonstrated by ranitidine. Another unknown mechanism able to trigger the neurohormonal program of migrating motor complex phase 3 or to abolish inhibitory influences should be taken into account to explain this effect of ranitidine.

Adult↗
PubMed1995

Abnormal esophagocardiac inhibitory reflex in patients with diffuse esophageal spasm.

The aim of this study was to establish if patients with an esophageal dysmotility similar to that found in the majority of patients with 'swallow syncope' may also have a pathologic esophagocardiac reflex similar to that observed in these patients. The effects on heart rate induced by dry and solid swallows and by intraesophageal balloon distension were studied in 8 normal subjects and in 10 patients with diffuse esophageal spasm by simultaneous recording of ECG and intraesophageal pressures. Dry swallows induced a brief increase in heart rate, while deglutition of a solid bolus initially induced an increase followed by a decrease in heart rate, significantly more marked in patients with diffuse spasm. The intraesophageal balloon inflation induced a decrease in heart rate which was significantly more intense in patients with diffuse spasm. In conclusion, the esophageal wall distension either due to solid bolus ingestion or to balloon inflation elicits an inhibitory esophagocardiac reflex which is more intense in patients with diffuse spasm and might induce cardiac arrhythmias in predisposed subjects.

Adult↗
PubMed1995

[Nonfamilial cherubism. A clinical case report].

The authors report a rare case of non-familial cherubism with a six-year follow-up. After an analysis of the hereditary transmission of the disease, the authors evaluate the anatomopathological and radiological characteristics and differential diagnosis, and then discuss possible therapies in the light of the latest research. They underline the important role of CT in identifying lesions which may not be diagnosed by simple X-ray with orthopantomography and highlight the importance of taking extreme care to conserve as much dental and bone tissue as possible during surgery.

Biopsy↗
PubMed1994

Differential hemodynamic, metabolic and hormonal effects of morphine and morphine-6-glucuronide.

Although the hyperglycemic effect of morphine has been previously described, it is not clear whether this is the result of increased glucose production and/or decreased glucose utilization and if this metabolic effect is lost with glucuronidation. This study assessed the hemodynamic (heart rate; HR and mean arterial blood pressure; MABP), hormonal and whole body glucose metabolic effects of morphine (MOR) and its metabolite morphine 6-glucuronide (MOR-6G) in conscious unrestrained chronically catheterized rats. Whole body glucose kinetics were assessed with a primed constant intravenous infusion of [3-3H]glucose in rats infused i.c.v. with H2O (Con; 5 microliters/h), MOR (80 micrograms/h) or MOR-6G (1 microgram/h) for a total of 4 h. MOR administration resulted in a significant 20% elevation in HR and no change in MABP. MOR-6G produced a 14% increase in HR and no change in MABP. A significant rise in plasma glucose (+23%), hepatic glucose production (Ra; +27-61%) and whole body glucose utilization (Rd; +31-61%) was also observed within 60 min of MOR administration. I.c.v. MOR-6G resulted in hyperglycemia (+60%), stimulation of glucose Ra (+60%) and glucose Rd (+50%). No significant alterations were noted in hemodynamic, metabolic and hormonal parameters of H2O infused rats. I.c.v. MOR resulted in a significant increases in epinephrine (2-fold), norepinephrine (50%), corticosterone (97%) with no alterations in plasma insulin and glucagon. I.c.v. MOR-6G resulted in more marked elevations in norepinephrine (5-fold), epinephrine (7-fold) and similar elevation in corticosterone (99%) and modest elevation of glucagon (40%).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1994

Antigravitropic activity of 2-(2-arylamino-1,2-dioxoethyl) benzoic acid methyl esters.

Methylation of 1,3-dihydro-1-hydroxy-3-oxo-1-isobenzofurancarboxamides (A-I) yields 2-(2-arylamino-1,2-dioxoethyl)benzoic acid methylesters. The esters 1-7 allowed the evaluation of the intrinsic activity of 2-(2-arylamino-1,2-dioxoethyl)benzoic structure, to whom the antigravitropic effect of the carboxamides A-I may be probably assigned. The comparison of activities of methylesters 1-7 (B) and 8-14 (C) shows how conformational differences as those between the arylamino-1,2-dioxoethylic portion of B and the arylamino-2-oxoethylic portion of C may deeply effect phytotropin interaction with the receptor site.

Benzoates↗
PubMed1991

[Salivary gland calculi with ambulatory treatment].

The surgical treatments suggested on selected patients are: --local anaesthesia, normally, when the calculus are located in the terminal seats of Wharton's and Stenone's duct; --general anaesthesia, as a rule, in surgical enucleation of the calculus seated up the duct or inside the gland.

Ambulatory Surgical Procedures↗
PubMed1991

["Esophageal" angina and angina pectoris].

In the last few years the non cardiac angina-like chest pain has encompassed more and more agitation not only in many patients but also in cardiologists, gastroenterologists and psychologists, as it involves socio-economic, pathophysiologic and therapeutic problems. The socio-economic aspect is well explained by the fact that in the USA at least 200,000 patients a year suffering from non cardiac angina-like chest pain, even when coronary arteriography has demonstrated normal coronary vessels, nevertheless continue to require cardiologic examinations and, if no one has clearly demonstrated the origin of their pain, they continue to live as invalids in constant fear of myocardial infarction. The discovery that the esophagus may be one of the causes of chest pain in these patients presenting with a previous diagnosis of "atypical" angina pectoris, unfortunately cannot resolve definitively the problem. An association of esophageal angina in patients with angina pectoris treated for long periods of time with Ca-antagonists and nitroderivatives has been described. In addition, the provocative or spontaneous tests to demonstrate the esophageal origin of chest pain give only a "likely" and not a "definite" diagnosis of esophageal angina. This also means to no "gold standard" text exist. Lastly, the "likely" diagnosis of esophageal angina is made in only about 50% of patients leaving the problem of the remaining 50% unanswered. These uncertainties induce some psychologists to assert that the cause of non cardiac angina-like chest pain is in the head ("panic disorder") and not in the esophagus, where the observed motor disorders should be an epiphenomenon.(ABSTRACT TRUNCATED AT 250 WORDS)

Angina Pectoris↗
PubMed1991

Determination of 2-mercaptopropionylglycine and its metabolite, 2-mercaptopropionic acid, in plasma by ion-pair reversed-phase high-performance liquid chromatography with post-column derivatization.

A simple and fast high-performance liquid chromatographic method was developed for the simultaneous measurement of 2-mercaptopropionylglycine (Tiopronine) and its metabolite (2-mercaptopropionic acid) in human plasma after the administration of a pharmaceutical dosage form (Acadione). The sample treatment before high-performance liquid chromatographic analysis consisted of the reduction of the corresponding disulphides by tri-n-butylphosphine and protein precipitation with ethanol. Separation was achieved by ion-pair high-performance liquid chromatography on a reversed-phase column (LiChrospher RP 18e) with cetrimonium bromide as counter ion and detection by fluorimetry after post-column derivatization with a selective thiol reagent, i.e. pyrenemaleimide. The high frequency of the analyzed samples and validation results make the method suitable for pharmacokinetic studies, and this was demonstrated by the first results obtained after the administration of an oral dose of 500 mg of Tiopronine to two healthy subjects.

Chemical Precipitation↗
PubMed1990

[Fibrous idiopathic hyperplasia. Report of a case].

It is presented a case of fibromatous gingival idiopathic hyperplasy of rare imponence. While describing the clinic characteristic the morphology of the lesion, the provoked functional troubles and the therapeutic program are evidenced. It is also confirmed its pathologic characteristic on hereditary base.

Adult↗
PubMed1985

Antimycotic 2-alkyldithio, 2-aralkyldithio, 2-aryldithiobenzamides.

Some 2-alkyldithio, 2-aralkyldithio, 2-aryldithio benzoic acids, their methyl esters and N-monosubstituted amides were prepared and tested in vitro against Candida albicans and Trichophyton mentagrophytes. Some N-monosubstituted amides displayed activities similar to those of clotrimazole and pyrrolnitrin. Against Candida albicans, N-monosubstituted amides exhibited a generally higher activity than the corresponding N-monosubstituted amides of 2,2'-dicarboxydiphenyldisulfide.

Amides↗