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M Mulczyk

Publications and source records attributed to M Mulczyk.

At least 37 records · Page 2Linked to original sources
PubMed1988

Outer membrane protein composition of Hafnia alvei.

The outer membrane proteins (OMP) obtained from 33 strains of Hafnia of different origin, were analysed by SDS-polyacrylamide gel electrophoresis. About 21 protein bands could be distinguished in OMP of each strain. The major proteins of the outer membrane were of apparent molecular weight 34.000 (34K), 37.000 (37K), 39.000 (39K) and 41.000 (41K). On the basis of the electrophoregrams obtained, four groups of strains possessing different composition of major OMP were distinguished: (1) 34K and 37K, (2) 34K, 37K and 39K, (3) 34K, 37K and 41K, (4) 34K, 39K and 41K. The major OMP of 34K present in all strains examined was susceptible to proteolytic enzymes and was found to be heat modifiable. The remaining major OMP of 37K, 39K and 41K were identified as peptidoglycan associated proteins.

Bacterial Proteins↗
PubMed1987

Hypersensitivity to Shigella outer membrane proteins in mice.

Mice sensitized with living Sh. flexneri 3a or with outer membrane proteins (OMP) derived from these bacteria showed Arthus and delayed type hypersensitivity (DTH) when injected in the footpad with OMP. The delayed reactivity to OMP was transferable to normal mice by spleen cells obtained from donor animals which were previously sensitized with living bacteria.

Animals↗
PubMed1987

Effect of slime extract from Pseudomonas aeruginosa on cell mediated immunity induced in mice by Salmonella typhimurium.

Immunity of mice intraperitoneally treated with slime extract and 24 h later intravenously immunized with live Salmonella typhimurium cells, was studied. The induced immunity was evaluated by determining the number of bacteria in the spleens and mortality of the animals. Slime extract in the dose of 200 micrograms/mice was found to suppress the immunity which manifested itself by enhanced proliferation of bacterial in the spleens and increased mortality as compared to control animals. The experiments on passive transfer of immunity against Salmonella typhimurium infection by splenocytes of slime extract-treated animals and then immunized, with those bacteria, revealed suppressive effect of the extract on transfer of the immunity. Similar results were obtained when splenocytes of mice sensitized with Salmonella typhimurium were given to animals pretreated with slime extract. The results obtained suggest the possibility of transfer of suppressive activity of splenocytes from slime extract-treated mice, to normal animals.

Animals↗
PubMed1987

Effect of outer membrane proteins of Shigella on delayed hypersensitivity in mice to sheep red blood cells.

Small doses of outer membrane proteins (OMP) of Shigella flexneri injected intraperitoneally into mice 1 to 3 days before or 3 days after sensitization of animals with sheep erythrocytes were found to increase delayed hypersensitivity as measured by the footpad reaction. In contrast, administration of higher doses of OMP resulted in suppression of hypersensitivity response. Cell transfer experiments showed that the spleen cells from sensitized and OMP treated mice transferred stimulating and suppressing activity to normal recipients. Suppression of hypersensitivity was also observed when recipients were injected with OMP 24 h before they were infused with spleen cells obtained from donor mice sensitized with sheep erythrocytes.

Adjuvants, Immunologic↗
PubMed1987

Some biological properties of outer membrane proteins of Shigella.

Outer membrane proteins (OMP) derived from two antigenically different representatives of Shigella (Sh. flexneri 3a and Sh. sonnei phase I) were tested for the toxicity, pyrogenicity, ability to induce Shwartzman reaction as well as for their influence on the leukocyte system. LD50 dose determined on mice was 28 mg/kg for OMP of Sh. flexneri and 23 mg/kg for OMP of Sh. sonnei. Both preparations injected intravenously to rabbits caused moderate increase of body temperature, expressed by the value 1.8 degrees C. Intravenous administration of protein preparations to rabbits, induced at first leukopenia and then transient leukocytosis. When injected subcutaneously in the dose of 500 micrograms and after 24 h intravenously in the dose 100 micrograms, they produced slight hemorrhagic changes at the site of administration.

Animals↗
PubMed1986

Humoral response in mice immunized with outer membrane proteins of Shigella flexneri.

Intraperitoneal immunization of mice with outer membrane proteins (OMP) of Sh. flexneri induced in the animals a synthesis of specific antibodies. Their level determined by ELISA test was found to be relatively low in the sera of animals immunized with a single dose (10 micrograms) of OMP; it was markedly higher in mice immunized with two doses of OMP, and very high after three fold immunization. The specific antibodies maintained in the animals for 8-16 weeks after immunization. Anti-OMP sera given to normal mice by intraperitoneal route protected them not only against challenge with homologous Shigella but also against Proteus and Escherichia.

Animals↗
PubMed1986

Characterization of outer membrane proteins of virulent phase I Shigella sonnei strains and their avirulent phase I derivatives.

Comparison of polyacrylamide gel electrophoretic protein profiles of four isogenic sets of virulent phase I Sh. sonnei strains and their avirulent phase I cells revealed no differences in outer membrane protein composition between virulent and avirulent derivatives. However, significant qualitative and quantitative differences were found in composition of major outer membrane proteins between strains of different origin. All four strains tested contained one major protein of 33K. This protein was susceptible to proteolytic enzymes and was found to be heat modifiable. Other major proteins of 35K and 37K present in three strains and 36K present in one strain were identified as peptidoglycan associated proteins.

Bacterial Outer Membrane Proteins↗
PubMed1985

Studies on specificity of protection induced by immunization with outer membrane proteins of Shigella.

Immunization of C3H/HeJ mice with outer membrane proteins (OMP) and with peptidoglycan associated proteins (PGP) isolated from Shigella flexneri 3a and from Shigella sonnei phase I protected the animals against lethal dose of homologous and heterologous bacteria and against various serotypes of Shigella flexneri. Neither of the protein preparations protected the animals against challenge with Escherichia, Klebsiella, Citrobacter, Salmonella, Serratia, Proteus and Pseudomonas. OMP preparations however, isolated from these species protected the animals not only against challenge with homologous bacteria but also against Shigella flexneri 3a.

Animals↗
PubMed1985

Transfer of immunity by means of spleen cells from mice immunized with outer membrane proteins of Shigella flexneri.

Intraperitoneal immunization of mice with a single dose (5 micrograms) of outer membrane proteins (OMP) of Shigella flexneri was found to evoke in the spleen the appearance of cells by means of which immunity to lethal dose of Shigella could be transferred into other mice. Active cells capable of transferring immunity appeared in the spleen of the animals as early as on day 3, reached the strongest protective activity on day 4 and disappeared on day 8 after immunization. Active cells from animals immunized with two doses of OMP maintained in the spleens for 19 days. The experiments revealed that immunity to Shigella could be transferred only with lymphocytes; macrophages were found to be inactive.

Animals↗
PubMed1984

Protective properties of peptidoglycan associated protein of Shigella flexneri.

Groups of CH3/Hej mice were immunized with peptidoglycan associated protein (PGP) from Shigella flexneri 3a administered in a single intraperitoneal injection (5 micrograms per mouse). After various periods post immunization the animals were challenged intraperitoneally with homologous bacteria. PGP proved to be strongly immunogenic. As early as one week after immunization all the animals survived challenge with 100 X LD100, and one third of the animals survived challenge with a dose of 500 X LD100. This high state of immunity maintained at almost unaltered intensity for 12 weeks after immunization (the end of the observations period).

Animals↗
PubMed1983

Antibacterial activity of Wratizolin.

Wratizolin in the concentration of 1-10 micrograms/ml was found to inhibit the growth of Gram-positive bacteria such as: Staphylococcus, Streptococcus, Sarcina, Corynebacterium, and some representatives of aerobic bacilli. Gram-negative bacteria of Enterobacteriaceae and Pseudomonas were not sensitive to Wratizolin even in the concentration of 100 micrograms/ml.

Anti-Bacterial Agents↗
PubMed1983

Antifungal activity of Wratizolin.

Wratizolin was found to inhibit completely or delay markedly the growth of fungi of Microsporum, Trichophyton and Epidermophyton genus. Mould fungi, yeasts, and yeast-like organisms, with a few exceptions, were not sensitive to Wratizolin.

Antifungal Agents↗
PubMed1983

Biological evaluation of Wratizolin penetration from ointments to the skin.

The degree of Wratizolin penetration to the skin from ointments prepared in 6 different media was determined. Experiments were carried out in mice and guinea pigs using: a) method determining the decrement of cytotoxic and antibacterial activity of the tested ointment contacted with the skin, b) biological tests to check the presence-of the drug in tissue extracts. Absorption of Wratizolin appeared dependent upon the kind of base ointment. The preparation penetrated well when suspended in polyethylene glycol base ointment (PEG).

Animals↗
PubMed1981

Studies on virulence of Shigella flexneri. Protective effect of outer membrane proteins.

Groups of mice were immunized intraperitoneally with proteins isolated from outer membrane of virulent strain of Shigella flexneri 3a and its avirulent variant. All the animals were found to survive challenge with LD100 of virulent Sh. flexneri 3a. Similar protective effect against challenge with Sh. flexneri 3a gave also immunization of mice with outer membrane proteins isolated from Sh. flexneri of other serotypes (2a, 4a, 6, and X) and from Escherichia coli, Hafnia alvei and Shigella sonnei.

Animals↗