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Biomedical subjects

M N Islam

Publications and source records attributed to M N Islam.

At least 19 recordsLinked to original sources
PubMed2006

T-cell sensitisation to hepatitis B virus surface antigens.

The present study was conducted to explore the immunity to hepatitis B surface antigens (HBsAg) of ad and ay subtypes at the cellular level among adult individuals. Peripheral blood mononuclear cells (PBMCs) obtained from acute hepatitis B virus (HBV) infected patients, chronic HBV infected patients, recovered subjects from HBV infection and uninfected vaccinated controls were stimulated with HBsAg ad and HBsAg ay subtypes in vitro. Stimulated PBMCs were incubated in CO(2) for production of interferon-gamma (IFN-gamma), which was measured from the supernatant of cultured PBMCs by an in-house ELISA technique. The mean +/- SE of IFN-gamma levels produced by PBMCs in response to HBsAg ad among the acute, chronic, recovered and control groups were 282.5+/-134.51 pg/ml, 307.45+/-94.84 pg/ml, 915.62+/-170.80 pg/ml and 511.67+/-161.22 pg/ml respectively, while on stimulation by HBsAg ay, the levels were 246.25+/-103.50 pg/ml, 374.70+/-104.02 pg/ml, 1040 +/-140.76 pg/ml and 465.83+/-166.26 pg/ml respectively among the above mentioned groups. The results of this study showed that PBMCs were non-responsive to stimulation by both HBsAg ad and HBsAg ay subtypes in acute and chronic patients with HBV infection. The recovered group responded significantly to both subtypes of HBsAg and the control group did not. The study indicates that although the patients with acute and chronic HBV infection showed weak or no IFN-gamma response to the HBsAg, subjects showed strong IFN-gamma response to the surface antigens on recovery from HBV infection.

Acute Disease↗
PubMed2006

Electrophoretic patterns of human rotavirus strains prevailing among hospitalized children with acute gastroenteritis.

Polyacrylamide gel electrophoresis (PAGE) and silver staining was applied to detect rotavirus dsRNA from acute diarrheic stool of 365 hospitalized children below 5 years of age. The study was conducted at Mymensingh and Sunamgonj districts, Bangladesh from January 2002 to February 2003. Among 345 stool specimens tested, 86 (24.9%) were positive by PAGE. The rate of infection was highest in 0-12 months of age and declined significantly with increasing age. Males were slightly more infected than females and infection rate was more in winter. Twelve different electropherotypes were identified, of them eight were long and four were short. RNA profiles of the analyzed specimens, 88.6% were long and 11.4% were short patterns. Two of these long patterns (2F N 1 L, 2F N 3(U) L) circulated through out the study period and a single type was predominant (2F N 1 L). Mixed electropherotypes were also detected. Electropherotyping technique can be applied routinely to study the prevalence and epidemiological features of rotavirus infection. It is an excellent method for studying genomic variation, tracing mixed infections, detecting atypical rotaviruses lacking group-antigen and characterizing virus strains in outbreaks.

Acute Disease↗
PubMed2006

Pattern of change of weight following birth in the early neonatal period.

Birth weight of new born will vary according to gestational age, maternal, Placental and foetal intrauterine conditions. Postnatal weight change in the first few days of life (early neonatal period) may vary according to gestational age and birth weight. This may create problem in management of these babies. Birth weight and their pattern of change were followed on 105 newborns. Questionnaire and observation charts were the research Instruments and statistical analysis were done by using manual calculator. It was observed that full term newborns had better control on initial weight change following birth than preterm and low birth weight babies. Majority of term newborns loss their weight up to 5 days of life and regained their initial weight by 10 days. Maximum weight loss for longer duration occurred in preterm and very low birth weight babies who regained their initial weight by 10 - 14 days and some required more than 21 days.

Age Factors↗
PubMed2006

Morphometry of the intercommissural distances and other structures of the aortic valve of bovine heart.

The morphometry of the intercommissural distances and their relation with other structures of the aortic valve of bovine heart was carried out. A total of 32 apparently healthy bovine hearts of indigenous cattle of both sexes (50% male and 50% female), 2-4 years of age, weighing 500 to 800 gms were used in this study. The external intercommissural distances were higher in all cusps of the valve compared to the internal intercommissural distances. The internal intercommissural distance was larger in the RC sinus in case of male animals followed by that of the LC and NC sinus. The mean external intercommissural distances were 34.67+/-3.40, 34.40+/-3.64 and 34.97+/-4.37 mm and the internal intercommissural distances were 21.60+/-4.21, 21.20+/-4.70 and 20.67+/-4.06 mm for LC, RC and NC respectively. Analyzing the internal intercommissural distance, value in the LC sinus was higher followed by the RC and the NC but this variation was non-significant. In regard to the position of the coronary ostia, there were two coronary ostia, the right coronary ostium present in the RC sinus and the left coronary ostium in the LC sinus respectively and the right coronary ostium was supracommissural in position in bovine heart. Data about the morphometrical analysis of intercommissural distances and the position of the coronary ostia of the aortic valve facilitate the knowledge of manufacturing bovine tissue valve as well as those might be involved in valve replacement in human.

Animals↗
PubMed2006

Serum triglyceride level in IUGR babies and its comparison with preterm AGA and term normal babies.

Intrauterine growth and birth weight are probably the most important factors that affect in survival and future quality of life. Intrauterine growth retardation causes program changes in body systems specially lipid metabolism that creates various problems of which coronary heart disease is important. Higher serum triglyceride (TG) level in IUGR babies relates to increase incidence of coronary heart disease. This cross sectional study was conducted in Bhangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during July 2004 to June 2005. Serum TG levels were estimated among three groups: IUGR (n=30), preterm appropriate for gestational age (AGA) (n=30) and term normal (n=30) new born babies. Blood samples were collected from the study population in the neonatal unit and serum TG level were measured with all accuracy in a computerized automated biochemical analyzer in Biochemistry department of BSMMU. Statistical analysis were done by using student's 't' test. It was observed that serum TG level (54.4 +/- 11.2 mg/dl) in IUGR babies were significantly higher than that of term normal babies (38.7 +/- 5.8 mg/dl), p value < 0.05. This implicate future coronary heart disease in these babies as shown by others in long term prospective studies.

Bangladesh↗
PubMed2006

Diagnosis of human rotavirus in stool specimens: comparison of different methods.

A total of 345 stool specimens of hospitalized children below 5 years of age with acute gastroenteritis were tested for the presence of rotavirus by Polyacrylamide gel electrophoresis (PAGE), a monoclonal antibody based enzyme immunoassay (EIA) and a latex agglutination test (LAT). Detection rate for PAGE and EIA were 24.9% (345/86) and 20.9% (345/70) respectively. Using PAGE as the standard, the sensitivity and specificity of EIA were 75.6% and 98.1% respectively. The sensitivity of LAT was 70.9% with 100% specificity (LAT was done in only PAGE positive specimens). LAT appeared as the simplest and economic for both bed side and field use.

Bangladesh↗
PubMed2006

Mitral stenosis in a girl of five years.

Mitral stenosis(MS) detected below the age of 20 years is called juvenile mitral stenosis (JMS). JMS constitute 25-40 % of all cases of isolated mitral stenosis, though overall incidence of rheumatic fever and rheumatic heart disease (RHD) within mixed population is 7.5- 7.8 per thousand. The patient was 5 years old girl hailing from Modhupur, Tangail got herself admitted into CCU Mymensingh Medical College Hospital with the complaints of low grade fever, shortness of breath and also associated with failure to thrive. She was ill looking, mildly anaemic. Precordial examination revealed apex beat was in the left 5th intercostals space, tapping in nature, palpable P(2). There was left parasternal heave. 1st heart sound loud, pulmonary component of the 2nd heart sound was accentuated, opening snap with mid diastolic murmur with pre systolic accentuation. Routine blood examination reveals leucocytosis with raised ESR. C-reactive protein (CRP) and ASO titre were significantly raised. X-ray chest P/A view showing the features of mitral stenosis. Echocardiography showing MS (moderate) with pulmonary hypertension. She was treated with antibiotics and other relevant drugs and discharge with an advice for follow up and take preparation for cardiac intervention.

Child, Preschool↗
PubMed2005

Topography and morphometry of the common bile duct and major duodenal papilla of man and principal domesticated animals.

A comparative topography and morphometry of the common bile duct and major duodenal papilla was studied on the extrahepatic biliary apparatus of human and major domesticated animals such as horse, ox, Black Bengal (BB) goat, sheep, dog and cat of local breed. In Black Bengal goat, sheep, dog and cat, the extrahepatic biliary system seemed to be the continuation of the cystic duct, which was found to continue as the common hepatic/common bile duct into which all the hepatic/lobar ducts open. In man, the mean length and the mean diameter of the common bile duct were 7.67+/-0.58 cm. and 7.27+/-0.77 mm. respectively. In the ox, these values were 1.57+/-0.72 cm. and 11.82+/-0.79 mm. respectively; in the Black Bengal goat, 7.23+/-0.74 cm. and 4.45+/-0.72 mm. respectively; in the sheep, 5.27+/-0.66 cm. and 3.75+/-0.79 mm. respectively; in the dog, 4.72+/-0.67 cm. and 6.43+/-0.55 mm. respectively and in the cat, 3.26+/-0.87 cm. and 5.63+/-0.43 mm. respectively. In man, the mean length and the mean diameter at the base of the MDP were 4.37+/-0.56 mm. and 3.97+/-0.72 mm. respectively. In horse, these were 52.36+/-0.67 mm. and 12.47+/-0.63 mm. respectively; in ox, 12.53+/-0.73 mm. and 8.83+/-0.68 mm. respectively; in Black Bengal goat, 14.36+/-0.55 mm. and 5.23+/-0.57 mm. respectively; in sheep, 11.27+/-0.69 mm. and 4.51+/-0.74 mm. respectively; in dog, 17.43+/-0.82 mm. and 6.72+/-0.64 mm. respectively and in cat, these values were 12.36+/-0.47 mm. and 7.12+/-0.87 mm. The distance of the Major Duodenal Papilla (MDP) form the pyloric end of the stomach was proportionately shorter in the meat-eaters, i.e., the omnivorous man (9.76+/-0.88 cm), and the carnivores, dog (6.37+/-0.73 cm) and cat (2.75+/-0.87 cm.) when compared to that of the herbivores, i.e., ox (55.37+/-0.68 cm.), BB goat (23.27+/-0.89 cm.) and sheep (25.62+/-0.77 cm.). In the horse, the major duodenal papilla was perpendicular to the duodenal mucosal surface with the largest mean length and the mean diameter. In all other animals, it was obliquely placed on the duodenal surface with its opening directed aborally.

Adult↗
PubMed2005

Effects of prolonged use of injectable hormonal contraceptive on serum lipid profile.

The current study was carried out to determine serum levels of total cholesterol, triacylglycerol (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in human female volunteers taking injectable hormonal contraceptives. For this purpose, 200 (two hundred) subjects with age range of 20-35 years were selected. Out of them, 140 (one hundred and forty) were experimental subjects and 60 (sixty) were control. Women without hormonal contraceptive were selected as the subjects of control group. The experimental subjects were taking injectable hormonal contraceptive, DMPA (depo-medroxyprogesterone acetate) for 3-5 years uninterruptedly. The mean serum total cholesterol and mean serum triacylglycerol levels of the DMPA users were significantly (P 0.05). The mean serum LDL-cholesterol level of the subjects of DMPA users was significantly (P<0.05) elevated in comparison to that of the control.

Adult↗
PubMed2004

The fuel properties of pyrolysis liquid derived from urban solid wastes in Bangladesh.

Urban solid wastes in the form of scrap tyre, waste plastic and waste paper, were pyrolyzed in an externally heated fixed bed reactor. The condensed liquids were analyzed for their properties as fuels and compared with petroleum-derived products. The properties were physical properties, higher heating value, elemental (CHNOS) analysis and chemical composition using Fourier transform infra-red (FTIR) spectroscopy. The results showed that the pyrolysis of urban solid wastes may be a potential source of liquid hydrocarbon fuel.

Bangladesh↗
PubMed2004

Reversion of molecularly engineered, partially attenuated, very virulent infectious bursal disease virus during infection of commercial chickens.

A molecularly cloned, tissue culture-adapted infectious bursal disease virus (BD-3tc) was generated from a very virulent strain by the reverse genetics approach following site-directed mutagenesis (Q253H and A284T in VP2). The pathogenicity of BD-3tc was tested in commercial chickens. The wild-type strain (BD-3wt) and the molecularly cloned parental strain (BD-3mc) were included for comparison. The subclinical course of the disease, with delayed and milder pathological lesions followed by quick follicular regeneration in the bursa of Fabricius in BD-3tc-inoculated birds, suggested that these amino acid substitutions made BD-3tc partially attenuated. However, severe bursa atrophy was observed at 14 days after inoculation. Reverse transcription-polymerase chain reaction coupled with restriction enzyme analysis revealed that both point mutations in BD-3tc had reverted 14 days after inoculation. Further investigations demonstrated that the codon for amino acid at position 284 had already reverted to the wild-type phenotype (T284A) 3 days after inoculation.

Animals↗
PubMed2004

Ciliary staphyloma: very rare sequelae of conjunctival rhinosporiodosis.

The main ocular manifestation of rhinosporiosis is lid or conjunctival infection. Conjunctival rhinosporiodosis is very rately causing sclera necrosis. A patient named Shumi (8 years), D/O.- Rukan Uddin of Rupchandrapur, Atpara, Netrokona admitted on 09/03/2003 in the department of Ophtalmology, Mymensingh Medical College Hospital with complains of mass in right eye looks externally like a growth in upper lid and mild dimness of vision of right eye. On examination, it was found that she got VAR : 6/24 and having a shaphyloma near 12-00 O'clock position with a mass adjacent to it which was identified as rhinosporiodosis after excisional biopsy. No history of ocular trauma or any other ocular disease was found.

Biopsy↗
PubMed2004

Iron status and infants feeding practice in children with anaemia.

A cross sectional study was carried out in the then IPGM&R now (Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh) to detect the pattern of feeding practice among the anemic children who were below 2 years of age and to find out a relationship between feeding practice and iron status. Hemoglobin was measured on finger prick blood samples using Haemoglobinometer. A peripheral blood film, and serum-ferritin was estimated by micro particle enzyme-immonoassay (MEIA). A dietary questionnaire was completed with particular emphasis on the type of feeding, breast and formula and at which age the weaning was introduced. 140 clinically suspected anemic patients were selected randomly for this study. Among them 111 (79.3%) patient had hemoglobin value ranging between 7.2-6.4 gm and their serum-ferritin level at or below 12 ngm/ml. It was observed that female children were predominantly affected and incidentally all patients were malnourished. In this study it was found that babies with an exclusive diet of breast milk were mildly anemic and not deficient in iron. On the contrary infants with mixed feeding habits or prolonged breast-feeding without weaning at all or babies devoid of any breast milk had moderate to severe anaemia and all were deficient in iron.

Anemia↗
PubMed2004

A study on interferon-gamma (IFN-gamma) response by T cells stimulated by hepatitis B virus core antigen.

Cell-mediated immune response by lymphocyte induced through recognition of HBV core antigen during acute HBV infection, chronic HBV infection and in subjects recovered from HBV infection was investigated in the present study by assessing the competence of IFN-gamma secretion by cultured PBMCs on stimulation by HBV nucleocapsid antigen (HBcAg). Fresh blood was collected in heparin from acute, chronic and recovered groups of HBV infected patients and uninfected vaccinated healthy controls aged between 18-50 years. PBMCs were separated by Ficoll-Hypaque density gradient centrifugation technique and were stimulated with hepatitis B virus core antigen (HBcAg) and mitogen (lectin). Stimulated PBMCs were cultured in CO2 and IFN-gamma levels were measured from the culture supernatant by an in-house ELISA technique. The mean+/-SE levels of IFN-gamma in HBcAg stimulated PBMCs in acute, chronic, recovered and controls groups were 875 pg/ml+/-297.56, 128.50 pg/ml+/-33.66, 905 pg/ml+/-172.51 and 235.33 pg/ml+/-111.28 respectively. IFN-gamma levels produced by HBcAg stimulated PBMCs of acute and recovered groups were significantly higher than that of chronic and control group (p<0.001). All groups responded strongly to lectin stimulation. Thus, it may be concluded that patients with acute HBV infection and those who had recovered from HBV infection show vigorous response to HBcAg stimulation in contrast to patients with chronic HBV infection.

Acute Disease↗
PubMed2004

Interferon-gamma (IFN-gamma) response to different hepatitis B virus antigens in hepatitis B virus infection.

The IFN-gamma levels in serum and cultured supernatant of peripheral blood mononuclear cells (PBMCs) were compared after stimulation by HBsAg ad, HBsAg ay and HBcAg among 3 groups of subjects i.e. patients with acute HBV infection, patients with chronic HBV infection and subjects recovered from HBV infection. Uninfected vaccinated group was taken as control. Serum and PBMCs were obtained from 38 individuals between 18-50 years of age. PBMCs were separated from heparinised blood by Ficoll-Hypaque density gradient centrifugation technique and cultured in CO2 incubator after stimulation by HBV surface and core antigens. IFN-gamma concentration was measured in serum and culture supernatant of PBMCs by an in-house ELISA technique. The mean serum IFN-gamma levels in acute, chronic, recovered and control groups were 88 pg/ml, 96.6 pg/ml, 155 pg/ml and 205 pg/ml respectively. On stimulation by HBsAg ad, IFN-gamma levels in cultured PBMCs of the above mentioned groups were 282.50 pg/ml, 307.45 pg/ml, 915.62 pg/ml and 511.67 pg/ml respectively, while in the same group on HBsAg ay stimulation, IFN-gamma levels were 246.25 pg/ml, 374.70 pg/ml, 1040 pg/ml and 465.83 pg/ml respectively. On stimulation by HBcAg, the IFN-gamma levels were 875 pg/ml, 128.50 pg/ml, 905 pg/ml and 235.33 pg/ml respectively in the acute, chronic, recovered and control groups. When compared with serum, significantly higher levels of IFN-gamma in cultured supernatant of PBMCs were observed after stimulation by HBsAg ad and HBsAg ay subtype in cases of chronic (p<0.05) and recovered groups (p<0.01 and p<0.001 respectively). However, no statistically significant difference of IFN-gamma level was observed between serum and PBMCs amongst the acute and control groups when stimulated by either of the HBsAg subtypes or HBcAg. In the recovered group, IFN-gamma levels produced by PBMCs after stimulation by HBcAg were significantly higher than that of serum (p<0.01). The study concludes that on subsequent exposure, PBMCs of the recovered group produces higher levels of IFN-gamma in response to different hepatitis B antigens. This response perhaps is able to protect individuals who are unable to develop anti-HBs.

Acute Disease↗
PubMed2003

Resistance pattern of bacteria isolated from acute respiratory tract infection (ARI) cases.

Out of 350 acute respiratory tract infection (ARI) patients 110 (31.4%) were positive on culture. Among then (35.5%) yielded from upper respiratory tract infection (URI) and 64.5% yielded from lower respiratory tract infection (LRI). Predominant bacterial isolates from URI were Staph aureus (12.4%) and Strepto. pyogen (9.8%) whereas predominant agent from LRI were Strepto. pneumoniae (14.7%) and Haemophilus influenzae (8.6%). Capsular typing by polymerase chain reaction (PCR) and type specific antisera revealed that 64.7% of the isolates were type-b and rest were non-b. The most frequent resistance pattern of H. influenzae was found resistant to penicillin-ampicillin (64.7%) followed by SMX-TMP (14.7%) and tetracycline (5.9%). Among penicillin-ampicillin resistant strains, 81.8% were beta lectamase positive and 18.2% were beta lectamase negative. Among beta lectamase positive strains 66.7% were capsular type-b and 33.3% were non-b, had MICS < or = 8 microgram/ml. and < or = 4 microgram/ml. respectively. 5.9% strains of Strepto. pneumoniae was found resistant to SMX-TMP, 5.9% to penicillin-ampicillin and 2.9% to cephalexin. Common resistance pattern of Staph aureus was penicillin-ampicillin (60%), SMX-TMP (37.2%) and tetracycline-erythromycine-cephalexin (11.4%). Strepto. pyogen was found resistant to tetracycline in 12.2% cases and to SMX-TMP in 8.3% cases.

Acute Disease↗
PubMed2002

Study of natural radionuclide concentrations in an area of elevated radiation background in the northern districts of Bangladesh.

The activity concentrations of naturally occurring radioactive materials in soil samples from an elevated radiation background area of three northern districts of Bangladesh were determined using gamma ray spectrometry. The outdoor and indoor external effective dose rates and the radiation hazard indices from these soil activities were evaluated. The dose rates were found to be about four times higher than the reported world average value. The concentration of natural radionuclides, derived radium equivalent activities and the representative level indices were also found to be higher. Recommendations on radiological and dosimetric measures have been suggested with an aim of minimising the harmful effects of ionising radiation to the population of the area concerned.

Background Radiation↗