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Biomedical subjects

M N Molodenkov

Publications and source records attributed to M N Molodenkov.

At least 37 records · Page 2Linked to original sources
PubMed1977

[Possiblity of using immobilized urease for degradation of urea in blood plasma].

As revealed the velocities of urea decomposition in the citrate donor plasma with soluble urease and urease immobilized by addition to carboxymethyl ester of cellulose, 2-(3'-amino-4'-methoxyphenyl)-sulphonylethyl ester of cellulose, diethylaminoester of cellulose, stained with dichlortriazine stain, or graft copolymere of cellulose and polyglycidylmetacrylate were sufficiently close to one another. Preparations of immobilized urease can be repeatedly used for urea decomposition in the citrate donor blood. Periodical treatment of the mentioned preparations with cystein solution led to a lesser decrease of enzymatic activity of the immobilized urease after repeated use.

Blood Preservation↗
PubMedDate not supplied

[Chemiluminescence of the blood serum in the presence of divalent iron salts].

On incubation of diluted blood serum with ferrous ions kinetics of chemiluminescence, typical for lipid containing systems, was observed. It comprised several sequental steps: quick flash, inhibition of the chemiluminescence, slow flash with subsequent steady-state luminescence. At each step of the phenomenon a correlation was found between the intensity of chemiluminescence, the rate of accumulation of products of lipid peroxidation (malone dialdehyde) and concentration of Fe2+ in the mixture. The data obtained suggest that chemiluminescence of blood serum in presence of Fe2+ is due to lipid peroxidation in the serum. Kinetics of the reaction and shape of the chemiluminiscence curve depended upon a number of factors: dilution of blood serum, concentration of Fe2+ added, temperature, pH and (if blood plasma was used) type of an anticoagulant use. Only in blood lipoproteins, mainly in beta-lipoproteins, the distinctive pattern of peroxidation was developed with simultaneous accumulation of malone dialdehyde, oxidation of ferrous ions and ultralow chemiluminescence. In protein fraction of blood serum addition of Fe2+ caused only the quick splash of chemiluminescence without the malone dialdehyde production.

Animals↗
PubMedDate not supplied

[Complex immunochemical examination of patients with some liver disorders].

Complex differential-diagnostic immunodiffusional test for a number of blood plasma proteins, antigens of liver tissue and alpha-fetoprotein was used to evaluate the liver tissue functional state. The test characterized more distinctly the liver tissue function as compared with estimation of individual proteins and was more informative in diagnosis of liver tissue disorders.

Antigens↗
PubMedDate not supplied

[Immunochemical identification of proteins adsorbed on columns of activated charcoal during hemosorption in patients with psoriasis].

After haemosorption of patients with psoriasis eluates from columns of IGI charcoal were tested for presence of 19 main plasma proteins using monospecific antisera. Distinct selectivity of protein sorption on IGI charcoal was found due to strong adsorption of the proteins responsible for immunity (IgG, IgA, IgM, C3- and C4-components of complement) as well as of fibrinogen.

Adsorption↗
PubMedDate not supplied

[Chemoluminescence of blood serum lipoproteins isolated by means of ultracentrifugation and precipitation with heparin and calcium].

Apo-B containing lipoproteins obtained after precipitation with heparin and calcium as well as very low (LPVLD) and low density lipoproteins (LPLD) produced using preparative scale ultracentrifugation had identical chemoluminescent properties. However, LPVLD are involved only slightly in total chemoluminescence of apo-B containing lipoproteins due to their low concentration in blood serum. In blood serum there is the factor having the quenching effect on the chemoluminescence and also exhibiting the antioxidant activity. Subfraction 3 of high density lipoproteins from patients with atherosclerosis differed from the analogous subfraction of donors by its chemoluminescent properties.

Calcium↗