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Biomedical subjects

M Ohkido

Publications and source records attributed to M Ohkido.

At least 55 records · Page 3Linked to original sources
PubMed1985

Effect of DNA-damaging agents on DNA replication and cell-cycle progression of cultured mouse mammary carcinoma cells.

The effects of DNA-crosslinking agents (mitomycin C and cisplatin), a DNA-intercalating agent (adriamycin) and monofunctional psoralen adducts (4-methyl-4',5'-dihydropsoralen plus near-UV radiation or 8-methoxypsoralen plus narrow band 395 nm light) on DNA replication and cell-cycle progression of cultured mouse mammary carcinoma cells were studied and compared at a dose of each agent sufficient to cause complete inhibition of cell growth. In cells treated with the DNA-crosslinking agents, inhibition of incorporation of [8H]thymidine occurred progressively upon incubation of the treated cells, and finally the cells were arrested at the G2 phase. However, although the mode of inhibition of DNA replication or cell-cycle traverse was the same as that of the crosslinking agents, adriamycin did not completely block cell progression at the G2 phase and some of the cells entered the G1 phase. In contrast to these agents, monofunctional psoralen adducts inhibited DNA replication immediately after treatment and no shift in the distribution of cells in the cell-cycle was observed during incubation. These results suggested different responses of the cells to different types of DNA damage.

Animals↗
PubMed1983

Contact dermatitis due to honeybee royal jelly.

A woman who had ingested honeybee royal jelly as a nutrient, showed an exacerbation of dermatitis when it was applied to her feet. A topical fungicide also aggravated her skin lesions. Patch testing showed positive reactions to the royal jelly, pyrrolnitrin in the fungicide and urushiol. Positive reactions to the royal jelly were found in 2 out of 10 controls, 1 of whom was sensitive to propolis.

Adult↗
PubMed1983

Mechanism of skin surface lipid peroxidation.

The correlation between the amount of lipid peroxide and squalene in skin surface lipid was observed by statistical analysis. To investigate the mechanism of skin surface lipid peroxidation, especially the role of active oxygen, squalene was irradiated by long wavelength ultraviolet light (UVA) with or without the photosensitizer, 8-methoxypsoralen (8MOP). Sodium azide (NaN3), a singlet oxygen quencher, or deuterium oxide, which elongates the life time of singlet oxygen, were also added in these experiments. The lipid peroxidation was detectable by observing malondialdehyde (MDA) formation. The MDA value in squalene solution was increased by UVA irradiation, and this effect was enhanced under the presence of 8MOP. These effects were repressed by the addition of NaN3 in the experimental system. These results may show that squalene peroxidation was mediated by singlet oxygen. Epidermal keratin could inhibit the increase of MDA values in human skin surface lipid in vitro. The inhibitory effect of epidermal protein on the deposition or the formation of lipid peroxide in skin surface was suggested.

Adult↗
PubMed1982

DNA replication and cell-cycle progression of cultured mouse FM3a cells after treatment with 8-methoxypsoralen plus near UV-radiation.

To investigate the response of cells to one type of DNA damage--namely DNA crosslinks--cell-cycle progression and macromolecular synthesis were studied with cultured mouse FM3A cells. Treatment of the cells with low doses of 8-methoxypsoralen (8-MOP) plus near-UV radiation (0.1 micrograms/ml plus 5 kJ/m2 or 1.0 micrograms/ml plus 1-2.5 kJ/m2) halted the progression of cells through the cell cycle temporarily for the first several hours. Then the cells resumed progression through the cell cycle, and most of the cells reached, and were finally arrested at, the G2 phase of the cycle. There was a rapid decrease of incorporation of [3H]thymidine into cellular Dna immediately after the treatment. Then, after 8 h of incubation, the incorporation of [3H]thymidine recovered to some extent depending on the dose of 8-MOP plus near-UV radiation. Thus the decrease and recovery of the incorporation of [3H]thymidine were correlated with the halt and resumption in the cell-cycle process. Synthesis of RNA and protein was measured by determination of the amounts in the cells or by the incorporation of radioactive precursors after treatment. RNA and protein synthesis were stimulated by low doses of 8-MOP plus near-UV radiation, but inhibited severely by high doses.

Animals↗
PubMed1981

Phytosterolemia and type IIa hyperlipoproteinemia with tuberous xanthomas.

A patient with phytosterolemia and type IIa hyperlipoproteinemia with tuberous xanthomas is described. The plant sterols, beta-sitosterol, campesterol, and stigmasterol, were identified in plasma and the xanthomas; to our knowledge, this is the first case of phytosterolemia and xanthomatosis in a Japanese.

Child, Preschool↗
PubMed1981

Some recent advances in HLA and skin diseases.

During the past 15 years, much has been learned about the human major histocompatibility complex. These new findings have been applied to the whole field of medicine, including transplantation immunology, blood transfusions, association with disease, human genetics, and the mechanism of human immune responses. In clinical medicine, the association between the major histocompatibility complex and various diseases has attracted the greatest interest. In this paper, we summarize the basic information on the human histocompatibility complex and its association with dermatologic disorders. Data from the world literature are reviewed and tabulated. Our own investigations on psoriasis, atopic dermatitis, and epidermolysis bullosa are discussed in detail.

Animals↗
PubMed1981

Studies on structural lipids in the stratum corneum.

To determine the mode of existence of structural lipids in the stratum corneum, lipids induced in the thick horny cell layers of guinea pig skin by n-hexadecane treatment were analyzed. Total lipids in the stratum corneum were extracted by Folch's solution and the ratio of phospholipids to free cholesterol was 1:8. Lipids extracted by non-ionic surfactant treatment appeared to originate from both the cell surface and intercellular space of the stratum corneum and the ratio of free cholesterol to phospholipids in this lipid fraction was much higher than that in the total lipids of the stratum corneum extracted by Folch's solution.

Animals↗
PubMed1980

Lipid peroxide of human skin.

Lipid peroxide in human skin surface lipids was investigated. The correlation between the amount of lipid peroxide and the total amount of lipids in skin surface lipids was determined by statistical analysis. Furthermore, the correlation between lipid peroxide and squalene in skin surface lipids was also proved. These results suggest that the major source of lipid peroxide in skin surface is squalene, and that lipid peroxidation is enhanced by external factors such as ultraviolet light irradiation.

Adult↗
PubMed1980

Two cases of chromomycosis.

More than 190 cases of chromomycosis have been reported in Japan since the first one in 1933. We encountered two cases of this fungal disease in the last four years. The diagnosis of chromomycosis is still difficult, especially in the early stage and brain metastasis is frequently seen in this country. We report here these two cases with a discussion on clinical manifestations of previous reports in Japan for early diagnosis.

Chromoblastomycosis↗
PubMed1980

Eight cases of sporotrichosis.

About 1,100 cases of sporotrichosis were reported in Japan between 1955 and 1973. From February 1975, when our hospital opened, to November 1979, we encountered eight cases of this disease. The patients, two males and six females, were aged from 6 months to 90 years old. Among the clinical types, three cases were of the lymphangitic type and five cases were of the localized type. Seven cases treated with oral potassium iodine and one case treated with topical thermotherapy were cured or improved by such treatments. We report here these eight cases with a discussion on clinical manifestations in previous reports of cases in Japan.

Adult↗