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Biomedical subjects

M Pandolfi

Publications and source records attributed to M Pandolfi.

At least 37 records · Page 2Linked to original sources
PubMed1984

Human ureter, a source of tissue plasminogen activator.

In organ cultures of human ureters, plasminogen activators were released into the medium. Unlike most tissue cultures that release mainly urokinase (UK), the activators released by ureter cultures were predominantly of the tissue-type (t-PA), as shown by radioimmunoassay. Using affinity chromatography it was possible to distinguish two activities. One minor fraction was quenched by antibodies against UK, but not by antibodies against t-PA. The other activity was quenched by antibodies against t-PA, but not by those against UK, and required the presence of fibrin to activate plasminogen. Because of the large amount of t-PA, cells derived from human ureter might well prove to be a useful source of t-PA.

Adolescent↗
PubMed1984

The effect of sodium hyaluronate and sodium chondroitin sulfate on the coagulation system in vitro.

Healon (sodium hyaluronate) and sodium chondroitin sulfate (CDS) are injected in the ocular cavities in a variety of operations, mainly intraocular lens (IOL) implantation. Both Healon and CDS are structurally similar to heparin. We found that like heparin CDS has an inhibiting action on blood coagulation in vitro. The inhibiting activity is of the antithrombin type. Healon does not possess anticoagulant activity. Since the anticoagulant effect of sodium chondroitin sulfate is observable at concentrations likely to occur in vivo the substance may impair ocular hemostasis.

Blood Coagulation↗
PubMed1984

Growth hormone and endothelial function during exercise in diabetics with and without retinopathy.

Changes in plasma growth hormone (GH), factor VIII related antigen (VIIIR:Ag), and plasminogen activator activity (PAA) during exercise were evaluated in 50 insulin-dependent diabetics. In patients with a short to moderately long duration of diabetes (5-19 years, mean 11), GH increased only in those with retinopathy. VIIIR:Ag and PAA increased most pronouncedly in retinopathy patients as well. In diabetics with long duration of the disease (21-49 years, mean 35), GH, VIIIR:Ag and PAA increased almost equally in those with and without retinopathy.

Adult↗
PubMed1982

Localization and characteristics of transglutaminase in the rabbit and human eye.

Transglutaminases (transamidases; endo-gamma-glutamine: zeta-lysine transferases) are calcium-dependent enzymes, which cross-link proteins by introducing covalent zeta-(gamma-glutaminyl)lysine pseudopeptide bonds between the molecules. The distribution and characteristics of transglutaminase in both human and rabbit eyes have been studied. Transglutaminase activity was measured with an isotope technique based on the enzyme catalysed incorporation of 14C-putrescine into casein. Electrophoretic characteristics were studied using agarose gel electrophoresis combined with a specific fluorescent activity staining procedure based on the transglutaminase catalysed incorporation of monodansylthiacadaverine into casein. A thrombin-independent enzyme, indistinguishable from the guinea pig liver transglutaminase with regard toi electrophoretic mobility, was found in the choroid/pigment epithelium and ciliary body of the human eye while the lens, retina, iris and vitreous humour did not contain detectable amounts. The lens and the choroid/pigment epithelium of the rabbit eye tissues contained high activities while the activity in the extract from the combined ciliary body/iris was low but measurable.

Aged↗
PubMed1980

Regulation of intraocular pressure and pupil size by beta-blockers and epinephrine.

The interaction of adrenergic beta-receptor blockers and epinephrine on intraocular pressure and pupil size was investigated in healthy volunteers. Oral (atenolol and propranolol) and local (timolol) beta-blockers were studied. The experiment was carried out under double-blind randomized crossover conditions. The results showed that the combination of oral beta-blockers with topical epinephrine had an additive hypotensive effect on IOP, while timolol and epinephrine eyedrops both reduced the IOP used alone, but combined had an antagonistic effect, resulting in the disappearance of the epinephrine-mediated IOP reduction.

Adrenergic beta-Antagonists↗
PubMed1980

[Ebstein's anomaly in adult patients: diagnostic and prognostic profile with regard to two cases (author's transl)].

Two cases of Ebstein's anomaly in apparently asymptomatic adult patients are described. The chances of non invasive recognition of the malformation by means of electro-vectorcardiography and echocardiography are discussed. Unlike their useful diagnostic contribution, their prognostic valve seems to be negligible. For this purpose clinical course of malformation would remain very important, even if some unusual electrocardiographic and radiological features can provide interesting informations.

Carotid Arteries↗
PubMed1980

[Maximal walking tolerance evaluated by graded treadmill exercise in femoral and iliac obstruction (author's transl)].

The Authors evaluated by graded speed treadmill exercise the maximal walking tolerance in two groups of patients with isolated obstruction of femoral (15 cases) and iliac (11 cases) respectively, recognized by non invasive methods. Clinical and instrumental patterns at rest were nearly the same. In the two groups studied the following data have been considered: ankle systolic pressure at rest, arm-thigh pressure gradient, pressure index, maximum walking tolerance and baseline pressure recovery time after exercise. Mean and standard deviation of each parameter were calculated; statistical significance of the observed differences was tested by Student's t test. Since walking tolerance resulted significantly higher (p < 0.01) in femoral obstructions, factors associated with the level of the obstructive lesion are analyzed and discussed, which can explain such functional behaviour.

Arterial Occlusive Diseases↗
PubMed1979

Hypotensive synergism of propranolol and adrenalin.

The effect of combined treatment with an oral beta-blocking agent (propranolol tablets 40 mg twice a day) and a local alpha- and beta-agonist (epinephrine eyedrops twice a day) was studied in 10 healthy volunteers. Propranolol was given in a double-blind randomized manner, while adrenalin was given unmasked. Propranolol and adrenalin proved to have an additive reducing effect on the intraocular pressure. The effect of propranolol alone was stronger than that of adrenalin.

Adult↗
PubMed1979

[VCG analysis of the delta wave in WPW syndrome: its relevance for localization of pre-excitation areas (author's transl)].

Electrocardiograms of 27 patients with the Wolff-Parkinson-White syndrome (12 A Rosenbaum and 15 B Rosenbaum type) were studied. Planimetrically it was determined mean polarity of the delta were on both frontal (DI and DIII bipolar leads) and horizontal (V1 and V3 precordial leads) planes. In order to identify anatomical site of pre-excitation, all data were statistically analyzed. It was allowed to distinguish 5 delta wave groups and 5 relative anatomical sites of pre-excitation: posterior paraseptal, left ventricle free wall, right ventricle free wall, left ventricle posterior wall and dorsal portion of the interventricular septum.

Adult↗
PubMed1978

In vitro release of fibrinolytic activators from cornea.

Rabbit corneas cultured in vitro in chemically defined media continuously released fibrinolytic agents. Such a substance was an activator of plasminogen and was released largely from the epithelium. Addition of prednisolone did not modify fibrinolytic activity, while death of the explants was followed by cessation of fibrinolysis, a finding suggesting synthesis of the activator during culture.

Animals↗
PubMed1978

Long-term treatment of glaucoma with systemic propranolol.

In 17 patients with glaucoma and in two with ocular hypertension, all of whom had not responded satisfactorily to any conventional therapy, treatment was extended to include propranolol systemically. In periods up to 3.5 years, this therapy has kept the intraocular pressure at levels below 23 mm Hg in seven patients. Propranolol was withdrawn in five patients whose visual field continually deteriorated. No correlation between worsening of visual field defects and decrease in systemic blood pressure was noted. In the other patients propranolol was withdrawn because of increasing intraocular pressure or side effects.

Blood Pressure↗
PubMed1978

Conjunctival bleeding in Osler's disease with associated platelet dysfunction. A case report.

A patient with Osler's disease (hereditary haemorrhagic telangiectasia) was admitted to hospital because of obstinate, profuse conjuctival bleeding occurring without any known preceding rrauma. Extensive examination of the haemostatic mechanism revealed an impaired platelet function reflected in defective platelet aggregation by ADP, collagen adrenalin and defective clot retraction. This platelet dysfunction, whose association with conjunctival telangiectasia was hitherto unknown, impaired the patient's already deficient primary haemostasis following the vascular anomaly and apparently contributed to the severity of the bleeding which could only be checked surgically. The findings seem to warrant investigation of the platelet function in patients with Osler's disease. In the event of platelet dysfunction drugs, such as acetylsalicylic acid (aspirin), indomethacin, dextrans as well as transfusions with bank blood are contraindicated.

Adenosine Diphosphate↗