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M Perraud

Publications and source records attributed to M Perraud.

23 records · Page 2Linked to original sources
PubMed1979

[Absence of anti double stranded DNA antibodies in viral hepatitis (author's transl)].

The presence of anti double stranded DNA antibodies (ds DNA Ab) in the sera of patients with acute viral hepatitis and B virus chronic active hepatitis has recently been described. In contrast with these results we found no ds DNA ab, but anti single stranded DNA antibodies (ss DNA ab) in the sera from patients with these diseases. The ds DNA ab were studied simultaneously by two methods: Immunofluorescence (IF) on crithidia luciliae and Farr test. In this later case it was shown that the ds DNA preparation contained no ss DNA because it did not react with an anti ss DNA rabbit serum. Our results indicate that the above mentioned authors have been using in their Farr test a preparation of ds DNA contaminated by ss DNA, thus revealing the presence of anti ss DNA ab but not anti ds DNA ab, in the sera of their patients. On the other hand the presence of ds DNA ab has been shown in the sera of 85% of the patients with systemic lupus erythematosus and of 20,5% of the patients with non EB chronic active hepatitis. At least one part of these later are probably autoimmune diseases.

Acute Disease↗
PubMedDate not supplied

[Intranuclear structures of monkey kidney cells recognised by immunofluorescence and immuno-electron microscopy using anti-ribonucleoprotein antibodies (author's transl)].

Autoantibodies which react with a ribonucleoprotein material present in the saline extract of nuclei have been found in the sera of patients with Sharp's syndrome and in some cases of systemic lupus erythematosus and of systemic sclerosis. Such antibodies give a granular or reticulated immunofluorescence staining of the nuclei of mouse peripheral blood leukocytes. When cultures of monkey kidney cells (Macacus cynomolgus) were examined by immunofluorescence using such antibodies, it was found that the staining pattern depended on the state of the cell cultures. Nuclei of cells in stationary cultures showed the presence of perinucleolar granules of RNP whereas, within a few hours after subculture, the nuclei contained widely dispersed specks of antigen. In mitotic cells the antigen appears to be evenly dispersed throughout the cell volume with the exception of the chromosomes. Immuno-electron microscopy of cells treated 24 hours after subculture indicates that the specks correspond to aggregates of 200--250 A granules, probably interchromatin granules.

Animals↗
PubMedDate not supplied

Comparison of three techniques for the detection of antibodies to double-stranded DNA: immunofluorescence on Trypanosoma gambiense, immunofluorescence on Crithidia luciliae and radioimmunoassay using the Farr technique.

Three techniques for the detection of antibodies against double-stranded DNA were compared: two immunofluorescent (IF) techniques using either Trypanosoma gambiense or Crithidia luciliae as antigen and a radioimmunoassay (RIA) based on the Farr technique. The IF on T. gambiense or C. luciliae gives very similar results but the reaction on C. luciliae is easier to read and to interpret. The use of C. luciliae permits the swift elimination of the two main causes of errors: antibodies against the nucleus of Trypanosomidae and antibodies reacting with the base of the flagella. The RIA appears as a very sensitive test since six sera gave a positive reaction with the RIA and a negative reaction with the IF on C. luciliae. However, the RIA gives in nine cases false positive reactions and in four cases false negative results. Those last four sera contained specific antibodies with low avidity for double-stranded DNA.

Animals↗