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Biomedical subjects

M Prinz

Publications and source records attributed to M Prinz.

At least 55 records · Page 3Linked to original sources
PubMed1996

Resolution of apolipoprotein B repeat unit position variants on agarose, denaturing, and native polyacrylamide gels.

Utilizing three different electrophoresis systems for the separation of variable number of tandem repeats (VNTR) alleles of the high variable region 3' from the apolipoprotein B (Apo B) locus, it could be shown that apparent allele subtypes on native polyacrylamide gels comigrated with the regular ladder alleles on agarose and denaturing polyacrylamide gels. Two subtypes, one with a shift towards the cathode and one shifted towards the anode, and the corresponding ladder alleles were sequenced in order to determine the molecular basis for this aberrant electrophoretical mobility. Subtype and ladder alleles displayed the same number of repeat units but differed in the number and order of different types of repeats. Depending on the variation in the region of pure AT repeat units, the potential secondary structures of hairpin loop formation differed for subtypes and ladder alleles, which is thought to explain the shift in electrophoretic mobility on native polyacrylamide gel electrophoresis.

Apolipoproteins B↗
PubMed1996

Consonant clusters in child phonology and the directionality of syllable structure assignment.

The production of target consonant clusters at early stages of acquisition is analysed from a phonological representational perspective. The data stem from five normal monolingual German and four normal monolingual Spanish children at ages from 0;9 to 2;1, observed in naturalistic settings. At the beginning stages, target clusters are reduced to a single consonantal position, due to lack of branching of the syllabic constituents. This finding coincides with other results in the literature, which have in general been explained by means of universal principles. Nevertheless, there is an essential difference between the German and the Spanish data: German children tend to prefer the first consonant and Spanish children the second one. This difference can only be explained in terms of parameterization of syllabification, which in German takes place from left to right and in Spanish from right to left. At later stages, when clusters begin to be produced with two consonantal positions, they offer evidence for the beginning of branching of syllabic constituents, due to parameterization, and for the chronological order of the setting of the subsyllabic parameters. Our data offer evidence in favour of the following acquisitional hierarchy: CV > CVC > CVCC > CCVCC.

Child↗
PubMed1996

Clear cell meningioma: report of a spinal case.

A sacrococcygeal case of clear cell meningioma in a 38-year-old man is reported. Compared to the seven spinal clear cell meningiomas described so far, this case demonstrated the highest recurrency rate. Multiple recurrences were most likely due to the extensive infiltrative growth pattern of the tumor hindering complete microscopic surgical resection. Histologic parameters were not relevant to predict recurrences, since cellular anaplasia was lacking and growth fraction was low in the first operation and all subsequent recurrences. In view of what is now known of clear cell meningiomas, the tumor showed all histologic and immunohistochemical features which are typical for this tumor entity. However, the tumor was first diagnosed by capable pathologists and neuropathologists as fibroma-like mesenchymal tumor, metastatic renal carcinoma, chordoma, chondroma untypical osteosarcoma, and microcystic meningioma. Correct diagnosis was made by electron microscopy which has revealed comparable findings to those of other rare electron microscopical cases reported so far; high content of cytoplasmic glycogen diffusely distributed throughout the cytoplasm or aggregated within vacuoles, intermediate filaments, desmosomes, interdigitation of cell membranes and large amounts of collagen fibers, some of which were of the giant amianthoid type. Since meningiomatous features of clear cell meningiomas are not obvious light microscopically and the tumors may be confused with nonmeningiomatous neoplasms, electron microscopical investigation is highly recommended in each case of suspected clear cell meningioma.

Adult↗
PubMed1994

High sensitive DNA typing approaches for the analysis of forensic evidence: comparison of nested variable number of tandem repeats (VNTR) amplification and a short tandem repeats (STR) polymorphism.

The approach of using nested primers for the APO B variable number of tandem repeats (VNTR) increases the sensitivity of the polymerase chain reaction (PCR) to single cell level. Different experiments and a comparison to the short tandem repeats (STR) system VWA were carried out, to determine the applicability of this method to forensic samples. Nested amplification of the Apo B VNTR was affected by a strong tendency towards preferential amplification of the shorter alleles. This phenomenon was observed for DNA quantities as low as 100 pg and impaired, depending on the allele length, the results for mixed samples. As expected, VWA polymorphism showed less preferential amplification. The high sensitivity of both PCR systems is accompanied by an increased susceptibility to contamination. Using artificially contaminated bloodstains, the bloodstain genotype, the contamination or both genotypes could be found on one piece of evidence. Here a single analysis can lead to an incorrect result. Therefore a strategy for obtaining reliable results should consist of multiple stain extractions and the amplification of different stepped dilutions of the DNA solution.

Base Sequence↗
PubMed1993

DNA typing of urine samples following several years of storage.

The possibility of typing DNA polymorphisms on urine samples was investigated in a controlled storage experiment and for samples that were 1-7 years old. Female urine samples showed a higher amount of epithelial cells and therefore a higher DNA yield. Employing the polymerase chain reaction, specific amplification results were achieved for all samples over a 6 month storage period. The microscopical examination of the samples revealed not only differing degrees of contamination with bacteria, yeasts and fungi, but also the presence of still intact epithelial cells. Only 20% of the male samples and 32% of the female samples yielded specific amplification results. By separating the human cells from the contaminating organisms prior to DNA extraction, the number of successfully typed samples could be improved to 35% of the male and 77% of the female samples. This result confirms that excess amounts of co-extracted non-human DNA can inhibit the specific amplification of human target sequences.

Cell Count↗
PubMed1993

Analysis of creatine kinase, CK-MB, myoglobin, and troponin T time-activity curves for early assessment of coronary artery reperfusion after intravenous thrombolysis.

BACKGROUND: Thrombolysis has become the standard therapeutic approach in patients with acute myocardial infarction. To identify patients who may benefit from early invasive procedures, reliable noninvasive assessment of success or failure of thrombolytic therapy is mandatory. METHODS AND RESULTS: In a prospective study in 63 consecutive patients undergoing thrombolysis for their first myocardial infarction, serial measurements of creatine kinase (CK), its isoenzyme CK-MB, myoglobin, and troponin T were done to determine their value for noninvasive prediction of coronary artery patency. Blood samples were drawn every 15 minutes during the first 90 minutes, every 30 minutes during the first 4 hours, every 4 hours during the first 24 hours, and every 8 hours during the first 72 hours. The perfusion status of the infarct-related artery was assessed angiographically 90 minutes after initiation of thrombolysis. For each marker, time to its peak concentration and its early initial slope (start of thrombolysis to 90 minutes thereafter) were determined. Areas under receiver operator characteristic (ROC) curves were 0.83, 0.76, 0.82, and 0.80 for maxima of CK, CK-MB, myoglobin, and troponin T, respectively (p = NS by univariate Z test). The corresponding values for early slopes of CK, CK-MB, myoglobin, and troponin T were 0.79, 0.82, 0.89, and 0.80 (p = 0.23 for comparison between myoglobin and CK-MB; p = 0.07 between myoglobin and CK). Sensitivity, specificity, and positive and negative predictive values regarding noninvasive prediction of coronary artery patency after 90 minutes were 80%, 82%, 95%, and 61% for time to CK maximum; 91%, 77%, 91%, and 77% for time to myoglobin maximum; 87%, 71%, 89%, and 67% for early CK slope; and 94%, 88%, 94%, and 82% for myoglobin slope, respectively. When myoglobin slope was assessed together with other clinical reperfusion markers (resolution of chest pain or ST segment elevation, occurrence of reperfusion arrhythmias) by logistic regression analysis, only the myoglobin slope was an independent predictor of coronary artery patency (p < 0.0001). CONCLUSIONS: With regard to noninvasive prediction of coronary artery patency after thrombolytic therapy, measurement of the early initial slopes of the serum markers within only 90 minutes after the initiation of therapy is as accurate as the determination of the time to their peak concentration. Compared with the other markers examined, myoglobin appears to have advantages because of its earlier rise, yielding a better negative predictive value and a higher area under the ROC curve for determination of its early initial slopes.

Adult↗
PubMed1991

[Experimental studies on intraligamental anesthesia].

In an experimental study on animals the correlation between the pressure created by periodontal ligament injection and the distribution pattern of the anesthetic solution was examined. It was possible to demonstrate the penetration following intraligamental injection by scintiscanning of the mandibular bone. A correlation between pressure and distribution could not be established.

Anesthesia, Dental↗
PubMed1990

The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains.

Bloodstains were made with 200 microliters blood on each of 11 different common substrates to examine the effect of the stain carrier on the amount and quality of DNA recoverable. High-molecular-weight DNA was extracted from all samples after 2 days. The yield of DNA from each sample varied considerably, not only between the different stain carriers but also within a given category. With a DNA yield of up to 10 micrograms, paper, glass, nylon, wood, smooth leather and wool gave the best results, followed by blue denim and wallpaper (up to 6 micrograms), cotton fabric and carpeting (up to 4 micrograms) and suede (up to 2 micrograms). For several stain carriers the DNA-containing solution was contaminated by chemical substances, which in the case of the blue denim, suede, and carpet samples inhibited the digestion of the DNA with restriction enzymes and prevented DNA typing. The different textures of the stain carriers tested and (as for varying yields on the same carrier) the differing degree of loss of DNA during extraction and the physiological variation in the number of leukocytes in human blood are discussed as possible reasons for the wide range of variation in the amounts of DNA it was possible to extract.

Blood Stains↗
PubMed1990

[Possibilities for individual identification of hair roots using DNA probes].

High-molecular weight DNA was extracted from hair roots. The obtained DNA was digested with Hae III and Msp I and transferred to nylon membranes by Southern blotting. The possibility of applying a probe for sex determination (pHY2.1) a "single-locus"-probe (YNH24) as well as a "multi-locus"-probe (M13) was examinated as a function of the amount of hair being supplied. In the laboratory experiment a single hair root was enough for a sex determination or individual identification. Because of methodical difficulties in practice this kind of profiling should only be done with several hair roots.

Adolescent↗
PubMed1989

DNA extraction from bloodstains in respect to age and stained substrate.

The amount and the quality of DNA that could be extracted from bloodstains equivalent to 200 microliters blood were examined after 3, 6, 9, 12, 15, 18 and 21 days of storage under dry or humid conditions at room temperature. DNA was also extracted from two days old 200 microliters bloodstains on different stain carriers. The amount of DNA that could be extracted showed a dependency on all the parameters that were examined. On carriers with a rough surface structure, where the blood can soak in, the resolving of leukocytes was impaired and the DNA recovery rate was low, e.g. carpeting and suede. The DNA yield was higher for substrates with a smooth surface, e.g. paper, glass and smooth leather. The aging experiments revealed that for the stains stored under dry conditions, there was no decrease in the DNA yield for stains on glass, while the amount of DNA that could be extracted became less for older stains on cotton. Under humid conditions the DNA yield was high and did not decrease for both glass and cotton fabric. The quality of the DNA was not impaired by aging or storage conditions, but for several stain carriers the DNA was contaminated by chemical substances which inhibited restriction enzyme digestion and successful DNA-typing.

Aging↗
PubMed1989

[Methodologic variants of DNA blood stain analysis: extraction in agarose and digoxigenin marking].

DNA was extracted from 1 microliter bloodstains on glass, cotton cloth and paper using proteinase K digestion in a solution containing low gelling temperature agarose. This method is advantageous for small amounts of source material because the purifying procedures of other methods that contain a risk of loss of DNA, are avoided. The recovered DNA was digested with Hae III and after Southern blotting, a Digoxigenin labeled probe (Amprobe, human Y-chromosome specific repeat) was successfully used for sex determination. With this non-radioactive method quantities of DNA smaller than 1.5 pg were detected.

Blood Grouping and Crossmatching↗
PubMed1981

[Vitamin A requirement of fattening turkeys. 1. Vitamin A requirement of fattening turkeys under conditions of intensive rearing on the ground].

In two experiments with a total of 2,250 fattening turkeys at VEB KIM Turkey and Goose Production Gühlen-Glienicke graded doses of vitamin A (0...20,000 I.U./kg feed) in a ration type poor in vitamin A and carotene were tested in dependence on sex. After a 20-day depletion period the vitamin A supplement of 0, 125 and 250 I.U.vitamin A/kg feed resulted in vitamin A deficiency symptoms after feeding this ration for 1 week. A supplement of between 500 and 1,000 I.U. vitamin A/kg feed diminished consumption in some cases significantly. On the basis of parameters of the fattening performance and of the vitamin A content of the liver it could be proved that the optimal growth of the male and female animals requires approximately 2,000 I.U. and the adequate storage of vitamin A in the liver approximately 5,000 I.U./kg feed. The requirements are fulfilled by the current supplements of the mixed feed for fattening turkeys kept intensively on the ground.

Adipose Tissue↗
PubMed1980

Asbestos as a cofactor in carcinogenesis among nickel-processing workers.

It has been widely assumed, especially in the absence of other explanations, that lung cancer and nasal sinus cancers observed among nickel smelter workers are the result of the carcinogenicity of nickel. Although there may be such influence, supplementary hypotheses are also possible. The nickeliferous ores from at least one major smelter in New Caledonia (excess numbers of cancers have been found in these smelter workers) are derived from serpentinized host rocks which contain large amounts of chrysotile asbestos. Analysis indicates that nickel ores from this area are heavily contaminated by these fibers. The deposits are mined for their nickel content, but workers may be exposed to the asbestos fibers contained in the deposits. Hygiene measures limited to the avoidance of nickel may be inadequate under such circumstances and should be reevaluated so as to prevent the inhalation of asbestos-containing dusts.

Humans↗
PubMed1980

[Requirements of turkeys kept on the ground with respect to energy and crude protein content of mixed feeds].

In four experiments with 9,500 turkeys assorted according to sex, three energy levels (500, 550 and 600 EFh (energetic feed units for hens = 14,6 kJ net energy body fat of hens)/kg feed) were combined with three levels of crude protein (250, 300 and 350 g/kg feed) in the mixed feed (9 rations) and tested under conditions of keeping the turkeys on the ground from hatching to their 42nd day of life. The feed consumption of the turkeys did not correlate with the energy content of the mixed feed. The energy consumption of the groups whose mixed feed had a high energy content (550 and 600 EFh) was significantly higher than that of groups whose feed had a low energy content. With the increasing content of crude protein in the mixed feed the consumption of crude protein per turkey and test period rose significantly. Due to the increase of the content of energy and crude protein, the live weight gain increased after a 28- and 42-day test period; the growth-stimulating effect was more distinct with male animals. Mortality had no connections with nutrition. For the starting-off feed for turkeys an energy content of < 580 EFh with 300 g crude protein per kg mixed feed up to the 4th/5th week of life is recommended.

Animal Feed↗
PubMed1978

[Energy and crude protein requirements of young fattening geese. 2. Inluence of varying energy and crude protein contents of feed on carcass quality].

In 5 trials using a total of 5,7000 geese (Deutsche Legegans maleXWhite Italian Goose female) of which 482 were slaughtered and subsequently dressed, the influence of varying net energy and crude protein levels in the ration on fattening and carcass performance were studied. Increased crude protein contents were found to improve the meat yield. High-energy feeding likewise caused gains to go up, the extra gain almost exclusively consisting in adipose tissue (particularly skin, and intestinal and abdominal fat). In addition, high energy intakes induced earlier juvenile moulting, this effect making the 56-day old carcasses rather bristly. Therefore, it seems more convenient to fatten geese on low-energy rations (500 energetic feed equivalents/poultry [EFp] per kg) up to the age of 63 days because the meat yield (especially of breast meat) is likely to improve considerably when slaughter takes place at a later date. Goose fattening rations should stick to the following parameters (per kg): 500--550 EFp, 160--180 g crude protein, 8 g lysine, 6.5 g thio-amino acids.

Animal Feed↗
PubMed1978

[Studies on the vitamin requirement and vitamin supply in fattening turkeys. 2. Checking the vitamin supplements to state-approved mixed animal feed for fattening turkeys].

In turkey fattening experiments, the vitamin A, vitamin D3, vitamin B6, vitamin B12, niacine, pantothenic acid, folic acid, biotine and choline chloride additions to mixed feeds for fattening turkeys were checked. The vitamin A requirement of the fattening turkey is met, in all stages of growth, by adding 3,000 I.U./kg feed. For vitamin D3 the necessary supplements are 1,000 and 500 I.U./kg for starter feed and fattening mixes, respectively. The requirements for vitamins B2, B6, B12, niacine, pantothenic acid, folic acid and biotine of the fattening turkey are covered by the respective amounts contained in the ration components. To ensure adequate choline supply (optimum gain, small losses) during the starter period, the addition of 500 mg/kg feed proved necessary. On the basis of the results presented, recommendations are given for requirement norms and mixed feed supplementation that have been fully implemented in the meantime.

Animal Feed↗
PubMed1977

[Requirements of Muscovy ducks (Cairina moschata domestica) for crude protein and energy levels of fattening diets].

Groups of male and female Cairina ducks subjected to equal starter feeding (1st to 3rd weeks) were used from the 4th week of live (fattening period) to test feed mixes of varying energy and crude protein levels (480 energetic feed equivalents/heu[EFH] - 140 g crude protein[CP]; 480 EFh - 180/g CP; 555 EFh - 140 g CP; 555 EFh - 180 g CP; 625 EEh - 140 g CP; 625 EFh - 180 g CP - all figures referred to 1 kg of feed). The different energy levels remained without influence of the live weight development. High-energy feed caused, however, higher expenditures of energetic feed equivalents per kg of gain and resulted in carcases with higher fat proportion. At all the 3 energy levels under study, high-protein feeding proved superior to low-protein rations with regard to live weight gains and meat yield. For the time being, 500 EFh and 180 g CP per kg of organic matter are recommended as indicators for Cairina duck fattening rations.

Animal Feed↗