Biomedical subjects

M Raes

Publications and source records attributed to M Raes.

69 records · Page 4Linked to original sources

PubMed1987

Transient synovitis of the hip in children: role of US.

Transient synovitis of the hip remains a common diagnostic problem for the clinician. The physical signs are not pathognomonic of the condition, and the classic technical examinations are of little help. Therefore, the authors retrospectively studied the value of hip arthrosonography in 46 children with clinical symptoms suggesting pathologic hip conditions. In 20 of the 21 patients with a final diagnosis of transient synovitis, articular effusion was detected on ultrasound (US). Conventional radiography showed an increased medial joint space in only eight of these patients. Increased echogenicity of the articular fluid was found in both transient synovitis and septic arthritis. The high sensitivity of US in detecting intraarticular fluid was demonstrated by cadaver studies.

Adolescent↗

PubMed1986

A new experimental model to study oxygen toxicity.

An experimental model was developed in order to study the protective effect of antioxidant molecules. Human diploid WI-38 fibroblasts were cultivated under 2 atm of 95% O2. Antioxidants like alpha-tocopherol or superoxide dismutase (SOD) were added respectively in the culture medium or directly inside the cell through a microinjection technique. With both antioxidant molecules a protection was observed. In the control experiment, cells died within 6 or 8 days depending on the confluency and the malonaldehyde content increased sharply. This model represents a new tool in order to test other antioxidant systems towards an oxidative stress.

Antioxidants↗

PubMed1986

The use of 131I-meta-iodobenzylguanidine in clinical diagnosis and staging of neuroectodermal tumors. Two case reports.

131I-Meta-iodobenzylguanidine has proved to be useful for the diagnosis of different kinds of neoplasms of neuroectodermal origin. The value of this scintigraphic method as a complementary technic to conventional diagnostic tests for staging in a child with neuroblastoma is described. In another child an ontogenetically related tumor, a mediastinal ganglioneuroma, was also visualized.

3-Iodobenzylguanidine↗

PubMed1985

Subcellular localization and modification with ageing of glutathione, glutathione peroxidase and glutathione reductase activities in human fibroblasts.

Differential centrifugation and isopycnic equilibration in density gradients were used to localize glutathione (GSH), glutathione peroxidase and glutathione reductase in the subcellular organelles of WI-38 fibroblasts. GSH was present in all the subcellular fractions, whereas the glutathione peroxidase and reductase activities were restrained to the cytoplasm and the mitochondrial fractions. After equilibration in density gradients, the results showed the presence of GSH, glutathione peroxidase and glutathione reductase in both the cytoplasm and mitochondria. GSH was also located in plasma membranes and probably in peroxisomes, endoplasmic reticulum and lysosomal membranes. Evolution of GSH in ageing fibroblasts showed a sudden increase of its concentration just before cell death. The glutathione peroxidase activity already decreases in the early passages, while the decrease of the glutathione reductase activity was constant and reached a drastic low level at the end of the culture. In conclusion, GSH is probably involved in the cell degeneration associated with ageing but because of its multiple functions and its ubiquitous localization, it is difficult to assert to which extent this metabolite is implicated in the ageing process.

Cell Line↗

PubMed1985

Alteration of enzymes in ageing human fibroblasts in culture. III. Modification of superoxide dismutase as an environmental and reversible process.

The alteration of superoxide dismutase (SOD) defined as the change occurring in its thermostability and observed in ageing cells, concerned the cytoplasmic but not the mitochondrial enzymes. Altered SOD disappeared if it was incubated in a supernatant from the young cells whereas supernatants from the old cells induced the alteration. The alteration induced on purified SOD was found to be associated with the appearance of thermolabile tetramers. Cytoplasmic SOD tetramers were also observed in the supernatants of the old cells. The addition of NADPH into the incubation medium could reverse this alteration; also when cultivated in the presence of vincamine the alteration, normally present in the old cells, disappears. The alteration of SOD is therefore associated with the formation of tetramers; it is a reversible process influenced by the cytoplasmic composition of the old cells.

Aging↗

PubMed1985

Enlarged persistent anterior fontanel following head injury. A case report.

A 2-year-old child is reported with an unusual large anterior fontanel. The boy sustained a head injury at the age of 10 months. Post traumatic skull X-rays showed a parietal bone fracture. At the age of 2 years a leptomeningeal cyst underlying the anterior fontanel was found on CT. A causal relation between the persistent anterior fontanel and the roentgenographic findings is discussed.

Arachnoid↗

PubMed1985

A boy with acromesomelic dysplasia. Growth course and growth hormone release.

A 2 6/12-year-old boy is reported with the typical clinical and radiological features of acromesomelic dysplasia. This rare skeletal dysplasia is inherited as an autosomal recessive trait, and differential diagnosis is to be made with pseudoachondroplasia and acrodysostosis. Endocrine investigations were performed, and their results are found to be normal. Longitudinal growth reveals a very early slowing down of growth velocity.

Blood Glucose↗

PubMed1984

Polyploid cells in ageing hamster fibroblasts in vitro: possible implication of the centrosome.

Fibroblasts from hamster embryos cultivated in vitro present the typical ageing process of other fibroblastic lines, but they also suddenly give rise to giant non dividing cells which could be considered to represent terminally differentiated cells [36]. We investigated the latter mechanism, first by showing that microtubules in these cells depolymerized from the centrosome and not from the cell periphery as in other cells; secondly we analysed the structure of the centrosome on serial sections and found a diminished pericentriolar material; finally time lapse sequence studies of cell division confirmed that this process sometimes aborts giving rise to these giant polyploid cells. As a consequence, what first appeared as a differentiation process is in fact the result of an environmental deterioration which probably reaches a critical level thus creating a catastrophic consequence for the cell.

Animals↗

PubMed1983

Ageing of hamster embryo fibroblasts as the result of both differentiation and stochastic mechanisms.

Fibroblasts from hamster embryos were serially cultivated in vitro and their evolution followed from a morphological, physiological and biochemical point of view. After an exponential growth for about 20 passages, cells entered the ageing phase which ended up after 29-34 passages. From our observations, it seems that the arrest of growth results from two different phenomena: first, the typically fibroblastic cells may undergo a stochastic ageing process; second, some of these cells evolve into a terminal differentiation process, characterized by a different non-fibroblastic phenotype.

Aging↗

PubMed1983

Microtubules and microfilaments in ageing hamster embryo fibroblasts in vitro.

Microtubules and microfilaments were investigated in hamster lung fibroblasts, during their in vitro life-span. These cells show a senescence process characterized by a drastic phenotypic change, resulting in two phenotypes: the type 1 cells, characteristic of young cultures and the type 2 cells appearing progressively with culture passages. Microtubules and microfilaments were observed at the TEM and also visualized by the unlabelled peroxidase-anti-peroxidase method. Moreover, the susceptibility of microtubules to nocodazole was tested in type 1 and 2 cells. We could not provide evidence for a different susceptibility to the drug. However the depolymerization wave occurred centripetally in type 1 cells whilst centrifugally in type 2 cells. These observations are discussed in relationship with the early arrest of division growth of the type 2 differentiated cells.

Animals↗

PubMed1981

The purification of plasma membranes from WI-38 fibroblasts: effects of ageing on their composition.

A three-step method for the purification of plasma membranes from WI-38 fibroblasts was developed thus allowing the recovery of 36--44% of the plasma membrane. Except in the case of galactosyltransferase, the activity of the contaminating enzymes was very low. Morphological observations confirm the presence of a homogeneous population of vesicles. Preparations obtained from young and old cell cultures were compared for their enzymatic and protein contents. With ageing the activity of 5-nucleotidase significantly increases whereas that of alkaline phosphodiesterase I decreases. Out of the 26 components detected after sodium dodecyl sulphate polyacrylamide gel electrophoresis, four decreased but only one increased. Cellular ageing seems to fulfil a specific and localized effect on the plasma membrane.

Cell Fractionation↗

PubMedDate not supplied

Alteration of enzymes in ageing human fibroblasts in culture. I. Conditions for the appearance of an alteration in glucose 6-phosphate dehydrogenase.

Glucose 6-phosphate dehydrogenase has been found to be altered in ageing human fibroblasts in culture. In this article, we have studied the effects of incubation conditions on glucose 6-phosphate dehydrogenase present in isolated cells or homogenates from young and old cells. We show that incubation at 4 degrees C and pH 7.4 induced the appearance of a heat-labile enzyme; this was not the case at pH 6.5. Also NADP+, when present, protects the enzyme from being modified. The kinetics of the modification indicates that the altered form of the enzyme is an intermediary stage between the active and inactive forms. We now have a model system in which the appearance of an altered enzyme can be induced in only a few hours; this model will be used in our next paper to study the mechanism of this alteration.

Cell Survival↗

PubMedDate not supplied

Alteration of enzymes in ageing human fibroblasts in culture. II. Conditions for the reversibility and the mechanism of the alteration of glucose 6-phosphate dehydrogenase.

The appearance of a heat-labile glucose 6-phosphate dehydrogenase fraction can be induced by incubation of human fibroblasts at 4 degrees C and pH 7.4. Using this system we first studied the effects of pH and NADP+ on the reversibility of the alteration. In young cells, the induced alteration of glucose 6-phosphate dehydrogenase disappeared if the pH of the medium was lowered to 6.5 or if NADP+ was added. In old cells, the disappearance of the natural heat-labile glucose 6-phosphate dehydrogenase was possible only if both pH 6.5 and NADP+ were present. Secondly, we studied the effects of the alteration and its reversibility on the equilibrium between the enzyme subunits; we showed that alteration and inactivation are linked to a dissociation of the enzyme subunits into inactive monomers. We also proposed a model where the heat-labile enzyme is a transient form between the active dimer and inactive monomer. Our results are in perfect agreement with the theories of post-translational modifications now proposed to explain the presence of the altered enzymes in old cells.

Cell Survival↗

PubMedDate not supplied

Cellular aging and the importance of energetic factors.

The in vitro aging of human fibroblasts has become a classical model for studying cellular aging. This model was lately redefined by showing that these cells represent a stem cell system in which they progressively pass through seven morphotypes. Experimental data showed that external conditions that can be considered as stresses for the cells, can modulate the genome expression by speeding up the passage of the cells from one morphotype to the other. In this article, we will interpret these observations from the point of view of the thermodynamics of far from equilibrium open systems, which shows the importance of the production and the use of energy, both responsible for the generation of a given amount of entropy production. In stable systems like these cell morphotypes, such a production is constant but external stresses can prematurely destabilize the steady state of entropy production and, in doing so, accelerate the process of aging. It is also predicted that cells submitted to a stress will use part of their energy in response to the stress. Some experimental data in favor of such an interpretation have been obtained and more will be presented here that show that both cell death and accelerated cell aging under stress are modulated by the level of energy metabolism. All theoretical and experimental arguments presented in this article will show that cellular aging is related to stress and also to energy production through a very elaborate system of regulatory processes necessary for the cell to survive and to perform specific functions according to its differentiated state. This regulatory system also permits the cell to adapt its response according to the intensity of external as well as internal challenges and one of these responses will influence the cellular aging rate.

Cell Death↗

PubMedDate not supplied

Involvement of microtubules in modifications associated with cellular aging.

Microtubules are ubiquitous cellular components involved in the control of cell structure and functions, such as cell division, regulation of shape and polarity, intracellular transport, etc. Consequently, any alteration affecting them in structure or function has a good chance of affecting the cell and generally leads to cell dysfunctions. This has been shown for instance, after treatment with microtubule-interacting drugs. Cellular aging is also characterized by the appearance of various cell dysfunctions, but the possible involvement of the microtubules in the aging process, although a rather tempting hypothesis, has not yet been extensively investigated. In this paper, I will first rapidly review the different components that build, organize and control the microtubules in normal cells, independently of the aging process. I will then consider the possible involvement of the microtubules in the aging process, more particularly in models of cells aging in vitro and in aging neuronal cells, which have been the most extensively investigated. There is some evidence for alterations in the microtubule organization both in cells aging in vitro and in the aging brain. But the interpretation of these data awaits further experiments, taking into account the latest progress in tubulin genetics and in microtubule biochemistry. Microtubules could also represent one of the cellular targets affected after signal transduction and could thus be involved in the resulting cellular responses. This hypothesis will be discussed, as it offers new insights into the regulation of microtubule organization, dynamics and functions in normal cells, which will be worthwhile to investigate during the aging process.

Animals↗