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M Schechter

Publications and source records attributed to M Schechter.

At least 91 records · Page 5Linked to original sources
PubMed1985

Further evaluation of lectin affinity purified glycoprotein (GP90) in the enzyme linked immunosorbent assay (ELISA) for diagnosis of Trypanosoma cruzi infection.

Sera from 143 patients considered to be infected with Trypanosoma cruzi on the basis of epidemiological, clinical and standard serological evidence gave positive results in the enzyme-linked immunosorbent assay (ELISA) using a lectin affinity purified 90,000 molecular weight glycoprotein (GP90) antigen preparation. Levels of antibody did not discriminate between clinically classified groups of patients in the chronic phase of infection. The GP90 preparation was found to be heterogeneous.

Adolescent↗
PubMed1985

Seniors in need.

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Aged↗
PubMed1984

Zymodeme and species specificities of monoclonal antibodies raised against Trypanosoma cruzi.

A series of hybrid cell lines was generated by the fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice that had been immunized or infected with Trypanosoma cruzi zymodemes (Z1, Z2, Z3 ). Four immunoglobulin isotypes, IgM, IgG1, IgG2a and IgG3 were represented amongst the monoclonal antibodies secreted by 22 hybridoma clones. On indirect immunofluorescence (IFAT) antibodies bound to flagellum, cytoplasm, cell membrane or stained the whole organism. Two antibodies were epimastigote-specific. Enzyme-linked immunosorbent assays (ELISA) and a dot immunobinding test were used to evaluate the zymodeme and species specificities of 13 antibodies: four reacted with all T. cruzi zymodemes tested, two reacted strongly with all except Z1, two predominantly with Z1, two predominantly with Z2, and three predominantly with Z3 . Two IgM antibodies cross reacted with Trypanosoma rangeli, T. brucei, Leishmania mexicana, L. braziliensis and L. donovani. Five antibodies were used in a preliminary immunobinding test, performed blindly, to compare monoclonal reactivities and zymodeme groups. The results suggested a correlation between the two methods of characterization. Anti-T. cruzi monoclonal antibodies are considered to have important applications to epidemiological studies and the improved diagnosis and prognosis of Chagas' disease.

Animals↗
PubMed1984

Speciality shops.

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Hospital Administration↗
PubMed1983

Purified Trypanosoma cruzi specific glycoprotein for discriminative serological diagnosis of South American trypanosomiasis (Chagas' disease).

Chagas' disease, leishmaniasis, and Trypanosoma rangeli infection are endemic and their distributions overlap in vast regions of South and Central America. Serological cross-reactivities can confuse epidemiological studies of these infections, and their differential diagnosis has been assigned a high priority by the World Health Organisation. A lectin-affinity-purified, 90,000 molecular weight glycoprotein (GP90) is present in the known principal strains (zymodemes) of Trypanosoma cruzi and absent from Leishmania and T rangeli. Patients with T cruzi infection have antibody to GP90, whereas patients with leishmaniasis do not and the two infections can be distinguished in an ELISA system using this antigen. In a mouse model, the same test can differentiate between T cruzi and T rangeli infections. Antigens purified by affinity chromatography clearly provide a practical basis for very precise, even strain-specific, diagnostic tests.

Antibodies↗
PubMed1978

Enhanced lymphocyte-mediated killing of tumour cells after tumour irradiation in vivo.

The effect of local X-irradiation of a syngeneic carcinoma of fibrosarcoma growing in the leg of W/Fu rats on the ability of host spleen lymphocytes to kill syngeneic and allogeneic tumour cells in vitro was examined. Lymphocytotoxicity was found to be enhanced one day after local X-irradiation (4000 R) when compared with unirradiated tumour-bearing rats (p = 0.05 to 0.01). This enhancing effect of local irradiation was not observed when the lymphocytes were tested 1 week or later after X-irradiation, nor when normal legs of non-tumour bearing rats were irradiated. Mechanisms which might possibly explain these results are a reduction in release of tumour-specific antigen which can act as an inhibitor of cell-mediated cytotoxicity, or depletion of suppressor cells in the lymphocyte population. These findings may be relavant to clinical studies of cellular immunity in patients undergoing radiotherapy of malignant tumours.

Animals↗