PubMed Health⌕ Search

Biomedical subjects

M Simić

Publications and source records attributed to M Simić.

At least 19 recordsLinked to original sources
PubMed2003

Preliminary assay on the antioxidative activity of Laurus nobilis extracts.

Antioxidative activity of Laurus nobilis leaves, bark and fruit methanolic extracts (crude and defatted) were studied on the level of lipid peroxidation in liposomes, induced by Fe(2+)/ascorbate system and measured spectrophotometrically by the TBA-test. The most significant inhibition of lipid peroxidation was obtained with methanolic extracts of laurel bark (70.6% of inhibition was obtained with 1.0 mg of crude extract).

Antioxidants↗
PubMed2002

Survey of natural and anthropogenic radioactivity in environmental samples from Yugoslavia.

The radioactivity of 238U, 226Ra, 232Th, 40K and 137Cs in sediments, soil, turf and honey from Serbia and Kosovo (Yugoslavia) was measured using gamma and alpha spectrometry in order to estimate the radiation hazard from natural and man-made sources, as well as to compile a database for radioactivity levels in those regions. One sample, collected in the vicinity of a "depleted uranium" (DU) shell of the recent Balkan war, revealed a high 238U activity and a non-natural 235U/238U activity ratio, confirming therefore its anthropogenic origin. However, some other soil samples coming from characteristic DU craters did not show any characteristic level of radioactivity. The other sediment and turf samples taken all around the country show low radioactivity levels for all the isotopes here considered. With the aim of obtaining some indication about radioactivity migration in the food chain, several honey samples have been examined too. All samples show very low radioactivity content, often indistinguishable from natural background.

Environmental Monitoring↗
PubMed2000

Attitudes, behaviour and knowledge on sexuality among female adolescents in Zagreb, Croatia.

The aim of this study was to estimate the level of knowledge about sexuality, attitudes and sexual behaviour of female adolescents. The study included 194 female students, 117 from Medical High School (MHS) and 77 from General High School (GHS) in Zagreb. Data was collected using an anonymous self-administered questionnaire. In addition to items on personal data (age, parental education etc.), the participants were asked to define terms about sexuality (e.g. menstruation, puberty) the definitions of which are found in biology textbooks for the fifth and eighth grade of primary school. The aim of the third part of the survey was to collect information about attitudes and behaviour of female adolescents. The results showed a low level of knowledge in students of both schools. General High School students showed a higher level of knowledge than their Medical High School peers. One fifth of General High School students and 1/3 of Medical High School students were unable to define the term "menstruation". The majority of adolescents talk about sexuality with their friends, 92.1% of General High School and 81.2% of Medical High School students. Almost 50% of students of both schools would like to talk about sexuality with their school doctor. 6.9% of Medical High School students had at least one sexual intercourse while none of the General High School students had been sexually active at the time of the survey. As the majority of students were not sexually active and results showed a rather low level of knowledge, this seems to be the ideal period for the implementation of educational programs aimed at increasing the level of knowledge, and thus preventing unwanted consequences (STD, pregnancy, abortion, infertility).

Adolescent↗
PubMed2000

Early diagnosis and treatment of ectopic pregnancy.

During the period from 1995 to 1999, 64 patients were treated for ectopic pregnancy. All patients admitted to the department passed the same procedure including (complete preoperative laboratory findings, Beta HCG, serum progesterone and transvaginal colour Doppler). In patients who had ultrasound finding typical for ectopic pregnancy in combination with positive Beta HCG, conservative treatment was primarily done. In the rest of the patients, Beta HCG was tested every second day and in combination with the clinical and vaginosonographical findings the patients underwent diagnostic or operative laparoscopy. Out of 64 patients 36 had visible ectopic pregnancy when admitted to the clinical department. Three patients had no visible ectopic pregnancy neither at the time of their admission to the department nor at the time of laparoscopy. One of them had cervical pregnancy and the other two had pregnancies in the uterine part of the tube. The patient with cervical pregnancy was treated with metrotrexat (MTX) 12 mg daily in 5 doses. One patient with cornual pregnancy was treated with high doses of oxytocin infusion in combination with MEB intravenously 3 x 1 amp. The other patient with cornual pregnancy underwent laparoscopy with cornual resection and salpingectomy. Four of other tubar pregnancies were treated with metrotrexat 12 mg/day for 5 days. Other ectopic pregnancies were treated as follows: 36 laparoscopic salpingectomies, 10 laparoscopic salpingotomies with ovum expression, 9 adnexectomies by laparotomy, and 2 laparoscopic adnexectomies.

Abortifacient Agents↗
PubMed1999

Removal of detergent and solvent from solvent-detergent-treated immunoglobulins.

The solvent-detergent (S/D) method was applied for inactivation of lipid-enveloped viruses during the production of immunoglobulins. Amberlite XAD-7 resin was used for removal of solvent (tri-n-butyl phosphate, TnBP) and detergent (Triton X-100) after the performed S/D inactivation procedure. The S/D reagents from the immunoglobulin preparation were adsorbed on Amberlite XAD-7, while immunoglobulins passed through the column and retained their biological activity. Using the method developed here, the final immunoglobulin preparation contains less than 1 ppm of Triton X-100 and less than 2 ppm TnBP.

Acrylic Resins↗
PubMed1999

Ion-exchange chromatography separation of the detergent and the solvent from immunoglobulins after solvent-detergent treatment.

For inactivation of lipid-enveloped viruses during the immunoglobulin production, the solvent-detergent (S/D) method was applied. Tri-n-butyl phosphate (solvent) and Triton X-100 (detergent) were removed from S/D treated immunoglobulins by ion-exchange chromatography on Q-Sepharose Fast Flow (FF). During the chromatographic procedure immunoglobulins remained bound on a Q-Sepharose FF, whereas solvent and detergent were eluted by washing with starting buffer. Elution of immunoglobulins was achieved by increasing the ionic strength of the starting buffer. The final immunoglobulin preparation contained less than 10 microg/ml of Triton X-100 and less than 2 microg/ml tri-n-butyl phosphate. It was confirmed that the S/D procedure did not cause a significant change in polymers and specific antibodies content. Immunoglobulin classes were also not affected by the same procedure.

Chromatography, Ion Exchange↗
PubMed1998

[Epizootiologic situation of rabies in 1997. Urbanization of sylvatic rabies in Yugoslavia].

The Federal Republic of Yugoslavia is situated on the border of a great sylvatic rabies epizootics occurring in central and eastern Europe. That is the reason why a relatively small number of rabid animals have been registered. In 1997, 124 rabid animals, mainly red foxes, were identified. In recent years red fox rabies epizootics in Yugoslavia has annually spread 15-20 km to the south. Rabies in domestic animals, especially dogs, was characterized with furious clinical forms and dog-to-dog transmission typical of urban rabies. Sylvatic rabies has been almost completely eradicated in some countries of western Europe in the last years, mostly due to the method of oral vaccination of foxes-the main vector of rabies.

Animals↗
PubMed1996

[Analysis of ischemic lesions of the liver after various periods of warm and cold ischemia].

Liver and sinusoidal wall cells become swollen very fast due to ischemia in liver allograft, with Eurocollins preservation solution. This process consequently narrows the sinusoidal lumen and significantly disturbs the "functional structure" of Rapaport microcirculation units. Advanced changes on "functional structure" microcirculation liver units can cause outflow block phenomenon of the liver and a consequential primary graft dysfunction. In order to avoid this unfavorable consequence of transplantation, it is necessary to assess the potential allograft vitality in advance. The assessment can be based on measuring values of sinusoidal volume, increase of the serum glutaminpyruvic transaminaze (SGPT) level per mass unit of liver tissue in the preservation solution and increase of necrotizing hepatocytes' number in liver lobules. During the first 8 hours of cold ischemia these values do not change significantly, so we can conclude that in this interval of cold ischemia liver is suitable for transplantation, which is not the case with warm ischemia where changes occur much earlier. Practically, the intensity of lesions with 4 hour period of warm ischemia equals the intensity of lesions with 16 hour period of cold ischemia.

Animals↗
PubMed1992

Circulating inhibitor of interleukin 1 activity in patients with chronic renal diseases.

The effects of sera derived from patients suffering from chronic renal diseases (endemic Balkan nephropathy, glomerulonephritis and pyelonephritis) on T cell proliferative response was studied. It was found that these sera contained factors which affect interleukin 1 (IL-1) dependent events in T cell proliferative response. The factors prevent costimulatory effects of IL-1 on T cells but do not bind to IL-1, nor do they affect interleukin 2 (IL-2) dependent T cell proliferation. These findings indicate that immuno-suppression observed in some kidney disorders may be partially due to serum immunoinhibitory factors affecting IL-1 activity.

Adult↗
PubMed1992

[Morphologic characteristics of the liver allograft in experimental warm ischemia].

Thirty-minute normothermic liver ischemia causes swelling of the hepatocytes and sinusoid wall cells, their individual necrosis and discharge of the glycogen supply in the hepatocytes. The swelling of the hepatocytes and of the sinusoid wall cells causes a significant reduction of the liver lobe sinusoid volume which decreases from 27.6% to 5.4%, representing, actually, one fifth of the normal value. Such finding was recorded as a critical vascular liver volume, because any further decrease of the sinusoid volume would inevitably lead toward manifest and persisting portal hypertension which evidently interferes with Bernoulli trial of the hepatitic perfusion. The obtained data determine morphofunctional relevancy of the liver allograft for transplantation and are useful for all other surgical interventions requiring partial resection of this organ or time limited regional and total ischemia.

Animals↗
PubMed1991

[The significance of synergism between cervical infections and prostaglandins in inducing preinduction priming in the 3rd trimester of pregnancy].

The length of labour and foetal trauma could be significantly reduced by reduction of cervical resistance in the cause of labour. It is well known that the most effective way of priming is by prostaglandins. Microorganisms of cervico-vaginal flora, with or without inflammatory component, can produce factors which can create predisposition for preterm delivery. Namely, increased levels of prostaglandins E2 and F2 alpha could be noticed in amniotic fluids, compared with these levels in pregnant women without signs of infection. The study included 60 parturients with low Bishop score (less then 5), with average gestational age of 40.5 weeks. Group A: Prepidil gel with 0.5 mg of prostaglandin E2 was applied to cause priming. Group B: Half of doses of Prepidil gel (0.25 mg) was applied to the 30 patients with clinical signs and with positive laboratory findings of cervical infection. After 12 hours all patients were induced with oxytocin. All obstetrically important parameters were closely followed in the course of priming and induction. Presence of cervical infection could be beneficial, because priming can be produced with half of doses of prostaglandin, which means medical (reduction of side-effects and dangers of hyperstimulation) and significant economical benefits regarding the high price of prostaglandins.

Adult↗
PubMed1991

[Morphologic analysis of damage in renal grafts in various preservative solutions].

In this study experimental research was conducted concerning the morphology of renal lesions, in the function of time (12, 24, 36 and 48 hours) on potential renal allografts, which were stored in various cooled preservative fluids. A physiological solution was used, Euro-Collins and modified Euro-Collins, where dextrose was substituted with mannitol in the quantity of 35 g/l. With the modification of the Euro-Collins solution, in other words by the substitution of dextrose with mannitol, better results were achieved, and the differences grow in the function of time. The electrolytic content of this solution successfully maintains the intracellular ionic balance, and mannitol, as a highly osmotically active substance, offers better protection of the cells of the nephron complex from intracellular water penetration, which immediately results in the prolongation of preservation time and better quality of cadaveric renal graft.

Animals↗
PubMed1990

[Damage in the hemato-enteral barrier in experimental liver transplantation].

Semiquantitative analyzes of intestinal mucous lesion graduating from 0 (normal) to V (necrotic) were done in the experimental model, 6-12h alter the heterotopic liver transplantation in dogs. All degrees of mucous damages were found in intestinal samples (mean value was 2.60). The most dominant were the lesions of III (31%) and IV (25%) degree while in 4% complete destruction of mucousal villious was observed. Success of liver transplantation besides the presence of the lesions of hepatic alograft during the phases of the warm and cold ischemia or immunological responses, depends on the changes in functionally connected regions. In that sense the damages of mesenterial department lead a very important role. Lumenal infective agents and cardiodepressive macromolecules, passing trough the hematoenteral barrier, cause local hepatic damages and central cardiovascular failure.

Animals↗
PubMed1990

[Morphofunctional changes in potential liver allografts in experimental conditions].

As a part of morphofunctional investigations of validations cadaveric grafts for transplantation evolution of the histomorphological changes was followed by the experiments on guinea pigs. Liver samples were immediately after sacrificing the animals and 1, 2, and 4h later and kept at body temperature, which corresponded to the conditions of "warm ischemia". Second group of samples was immerged in Saline are +4 degrees C (hibernation-preservation) and was taken for analysis in same intervals as previous ones. Evolution of morphological and enzymatic changes was evident in short interval after sacrificing the animals. First changes were observed in cytoplasm and then in nuclei and interestitum. Changes of hepatic cells from hibernation preservation group were significant damaged comparing with the corresponding samples obtained from cadaver. ("warm ischemia"). These preliminary results indicated that length of liver graft preservation is very limited. Furthermore, this results demand for the future investigations in different conditions of preservation.

Animals↗