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Biomedical subjects

M Singh

Publications and source records attributed to M Singh.

At least 1,099 records · Page 61Linked to original sources
PubMed1982

Kala-azar in Afghanistan.

The first three cases of kala-azar from Afghanistan are reported. Two girls (aged 4 and 5 years) and a 5-year-old boy reported from Kabul and Badghis Province with classical features of visceral leishmaniasis. The diagnosis was based on the demonstration of amastigotes in the bone marrow.

Afghanistan↗
PubMed1981

Zinc, copper and manganese levels in various tissues following fluoride administration.

Distribution of zinc, copper and manganese has been studied in liver, kidney and bone of rats subjected for 10 months to varied fluoride concentrations in drinking-water. In the liver a significant fall in the levels of Mn, Cu and Zn was registered. In the kidney, the Mn level fell whereas the zinc level increased. In the bone, the copper content fell, whereas the manganese content increased.

Animals↗
PubMed1981

Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Efficient mechanised synthesis of heptadecadeoxyribonucleotides has been achieved on an economically small scale by an improved solid phase phosphotriester method on a polydimethylacrylamide resin. Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.

Base Sequence↗
PubMed1981

An insulin delivery system from oxidized cellulose.

Cellulose oxidized with periodate (OC) has been shown to be a biocompatible, biodegradable matrix suitable for immobilization and sustained release of drugs. In the present work, insulin was immobilized on the OC-matrix and the release profile in vivo and in vitro was examined. The hormone is bound to the matrix by Schiff's bond formation and is released by hydrolysis of this bond as well by degradation of the OC-matrix. The release can be sustained over longer periods by reducing the Schiff's base with NaBH4. The insulin depots could be conveniently injected as suspensions and are able to maintain glucose levels in diabetized rabbits at normal values over several days.

Animals↗
PubMed1981

Zinc Depletion following experimental fluorosis in mice.

The effect of fluoride administration on zinc levels in liver, kidney and bone, and on protein content (microgram/mg tissue wt) in liver and kidney was investigated by subjecting albino mice for 16 weeks to different concentrations of fluoride (0 ppm - control; 10 ppm - group I; 25 ppm - group II; 50 ppm - group III; 100 ppm - group IV; and 200 ppm- group V) in drinking water. Zinc levels decreased significantly in liver and bone of groups III, IV and V; the decrease being 17%, 25% and 44% (P less than 0.005) in the case of liver, and 14% (p less than 0.01), 24% (p less than 0.005), and 39% (p less than 0.005) in the case of bone in groups III, IV and V respectively. Zinc depletion in the kidney of the fluoridated animals was only statistically significant (p less than 0.005) in groups IV and V. Similarly the protein content also decreased in liver and kidney (in liver significant at p less than 0.005, p less than 0.01, and p less than 0.005; in kidney significant at p less than 0.005, p less than 0.05 and p less than 0.005 in groups III, IV and V respectively). The experimental animals, especially in groups IV (100 ppm F) and V (250 ppm F), also exhibited retardation in growth.

Animals↗