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Biomedical subjects

M Spaczyński

Publications and source records attributed to M Spaczyński.

At least 19 recordsLinked to original sources
PubMed2006

The level of antibody against E6 HPV 16 oncoprotein in blood sera of women with chronic HPV 16 infection and cervical cancer.

UNLABELLED: The aim of this study was to estimate of the role of chronic HPV 16 infection and the presence of anti E6 HPV 16 in the initiation of the cancerogenesis process of cervical cancer. MATERIAL AND METHODS: The study included two groups of patients. The first group comprised 323 women observed for three consecutive years (1998-2000), in whom the presence of HPV 16 viruses was estimated by PCR, and the level of anti E6 HPV 16 antibodies was estimated in the plasma with ELISA. A similar test was performed in a group of 46 patients with cervical intraepithelial neoplasia (CIN), 91 patients with invasive cervical cancer and 22 women after hysterectomy and RTG-therapy. RESULTS: In 32 patients, chronic HPV 16 infection showed a steady rise in the mean absorbance level of anti E6 HPV 16 antibodies from 0.04 in 1998 to 0.06 in 2000, while in HPV-negative women the mean absorbance value was 0.03-0.04. Mean absorbance value in patients with CIN III and invasive cancer rose with advancing stage of the cancer process and lowered after completion of oncological treatment. The values were 0.14, 0.33 and 0.13, respectively. CONCLUSION: The persistence of chronic HPV 16 infection and accompanying steady rise in absorbance index caused by an increase in the level of antiviral antibodies are a clear warning signal preceding in time the histological process of cancerogenesis.

Adult↗
PubMed2001

[Integrin alpha-6 in cervix neck squamous cell carcinoma HPV 16/18 positive and negative].

In the present paper we study expression of alpha-6 integrin in normal paraepidermal epithelium, in CIN and in cervical invasive carcinoma HPV 16/18 positive and negative. The results suggest that alpha-6 integrin might be a marker for the evaluation of malignancy of squamous cell carcinoma. There is no correlation between the expression of alpha-6 integrin and the presence of HPV.

Antigens, CD↗
PubMed2001

[Ovarian cancer and endometriosis].

Endometriosis is a relatively common condition found in up to 29% of women undergoing laparotomies. The authors suggest that endometriosis could have undergone malignant change. They propose the criteria used to establish that a malignant tumor has develop in endometriosis: clear evidence of endometriosis should be found close to the tumor, the histopathological appearance should be such that origin of the tumor from endometriosis is plausible, no other primary site should be found. The authors consider that atypical endometriosis possesses a precancerous potential or is most frequently associated with endometrioid and clear cell carcinomas.

Adenocarcinoma, Clear Cell↗
PubMed2001

[Gynecologic endoscopy in the greater region of Poland during 1988-1998].

The aim of the study was to analyze the state and development of the gynaecological endoscopy in the Wielkopolska region in years 1988-1998. The questionnaire included gynaecological and obstetrical departments of 31 hospitals: 1 academic center, 5 former regional hospitals and 25 small hospitals. At the end of 1998 35.5% of departments were equipped with laparoscopes and 9.7% with histeroscopes. There was an increase in the laparoscopic procedures from 151 to 832 estimated in these years, as well as from 181 to 314 hysteroscopies. The detailed analysis of procedures and dynamic of its increase according to the type of hospital is presented. The experience of the staff was also analyzed. We demonstrated the insufficient equipment in the region, in spite of the right tendencies in the spectrum of the endoscopic procedures and the experience of the physicians who perform laparoscopy and hysteroscopy.

Adult↗
PubMed2000

[Bone marrow involvement in ovarian cancer determined by immunohistochemical methods].

Atypical epithelial cells in the bone marrow of patients with ovarian cancer were evaluated using immunohistochemical techniques. We investigated cytospin preparations of bone marrow taken from 9 women with benign ovarian tumors and 59 women with malignant ovarian tumors. Two monoclonal antibodies (NCL-C11 and NCL-CA 125) were used. With both antibodies we were able to detect keratin and CA 125 antigen expression in the bone marrow of 9 (18.4%) of the patients with ovarian cancer. With regard to the wide histological differentiation of ovarian carcinomas, the presence of atypical epithelial cells in the bone marrow was required as a prognostic factor for survival and relapses. This should be investigated in a larger study group.

Adult↗
PubMed2000

[Immunohistochemical examination oncogenic c-erb-b2, egf-r proteins and antioncogenic p53 protein in vulvar cancers HPV-16 positive and negative].

In vulvar cancers HPV 16 positive, HPV 16 negative, and vulvar and vaginal precancerous status (VIN, VAIN) immunohistochemical technik onko- and antioncogenic proteins were evaluated. The contrary correlation between HPV 16 presence and overexpression p53 were detected. It suggest the heterogenic etiology of these cancers. There was payed attention to high activity of virus replication and intensive virion production in VAIN. Nontreated vaginal lesion may make difficult to obtain a positive cervical HPV infection treatment.

Adult↗
PubMed2000

Relationship between HPV infection of the cervix and blood serum levels of steroid hormones among pre- and postmenopausal women.

Human papillomaviruses (HPVs) are frequently present in anogenital lesions but little is known about their role in carcinogenesis. There are steroid hormone response elements in virus genomes that influence expression patterns of viral genes. Activity of the elements may contribute to development of neoplasia in case of hormone level anomalies. Our study was to determine whether the presence of HPV DNA in cervical smears correlates with abnormal levels, of steroid hormones in blood serum. One hundred women aged 40-62 participated in the tests and were divided into two groups: premenopausal and postmenopausal (45 and 55 individuals, respectively). Presence of HPV DNA in cervical smears was detected by PCR and Southern blot hybridisation. Progesterone and estradiol levels in blood serum were measured by radioimmunoassay. Our study showed a higher prevalence of HPV DNA in women with higher levels of progesterone in blood serum. A relationship between hormone level and HPV DNA prevalence should alert clinicians about using hormone contraceptives and hormone replacement therapy.

Adult↗
PubMed2000

[Chorion carcinoma: failure of treatment].

Between 1986-1998 in University Oncology Gynecology Department in Poznan, Poland were treated 23 women with choriocarcinoma. Despite of intensive chemotherapy 3 women were dead. In the report we present the history of this choriocarcinomas making effect to answer why our therapy was ineffective.

Adolescent↗
PubMed2000

[Gestational trophoblastic disease: the epidemiological analysis of 342 cases].

Between 1987-1996 dates were collected to assess frequency and risk factors for gestational trophoblastic disease in a case-control study of 342 women with trophoblastic tumors and 342 pregnant women admitted for deliveries or spontaneous abortion to University Hospitals in Poznan, Poland. Were analyzed the age of women obstetric history, place of live and repeat appearance of hydatidiform mole. The risk of trophoblastic disease increased with increase in maternal age and above third pregnancy. The risk independent of living in town or in the country. The second and more incident of hydatidiform mole was associated with greater risk of malignant sequele. The study of the pregnancy of gestational trophoblastic disease was led in Great Poland in the support on the date from all pathologic centres in this region and public demographic office. The frequently of hydatidiform mole was between 1987-1996 2.32 per 100,000 women, and 0.76 for 1000 live birth (1 HM for 1315 live birth). The frequently of choriocarcinoma was 0.08 per 100,000 women (and 0.38 per 10,000 live birth (1 CHA per 26,315 live birth).

Choriocarcinoma↗
PubMed1999

[Bone marrow involvement in ovarian cancer by immunohistochemical assessment].

In the study below we introduced certain conclusions from bone marrow analysis in patient with ovarian cancer in the presence of epithelial cells. The study included cytological bone marrow slides taken from 31 patients. We analyzed the cellular antigen expression using immuno-histological chemistry technic with NCL-CA 125, NCL-EMA and NCL-C11 antibodies. In the material drived from 3 patients with advanced neoplastic changes, we detected the presence of cells that were stained positively. In order to define the actual frequency of cell presence with tested antigen expression in bone marrow as well as to signify the diagnostic and prognostic value of their detection requires a larger study group as well as using other examination technics such as PCR or transluscent photometry.

Adult↗
PubMed1998

[Bowen's disease: one of the types of vulvar intraepithelial neoplasm (VIN) III (four case reports)].

Vulvar intraepithelial neoplasia has been considered a problem of postmenopausal years, but its frequency appears to be increasing among younger women. Bowen's disease is a form of VIN III carcinoma in situ, that may appear clinically as pigmented lesions. This pathology mostly concerns younger women, and is probably connected with human papilloma virus (HPV) infection. The incidence rate is very rare. During the last 15 years only four cases of Bowen's disease have been diagnosed and treated in Division of Gynecological Oncology in Poznań. In this paper we presented symptoms, diagnostic procedures, results of clinical and histopathologic evaluation as well as managements methods. Also the results of treatment and reports on follow-up visits have been discussed.

Adult↗
PubMed1998

Malignant melanoma of the vulva: 2 case reports.

It was described two cases of vulvar melanoma malignum. Both women after primary radical treatment, unfortunately died. It was discussed the classification of melanoma malignum, the efficiency of treatment nowadays and the problems in diagnosis. General results of treatment are poor. It is due to tendency for spreading in early stage of this neoplasm.

Aged↗
PubMed1998

[Non-neoplastic epithelial disorders associated with vulvar carcinoma].

The purpose of this paper has been clinical problems associating squamous cell carcinoma of the vulva co-existing with non-neoplastic epithelial disorders. We analyzed 46 women with vulvar carcinoma. Non-neoplastic epithelial disorders we found in 17% cases. In conclusions we suggest the follow-up in patient with non-neoplastic epithelial disorders must be held the same like with vulvar carcinoma.

Adult↗
PubMed1997

[Markers of proliferation and morpho-immunologic parameters in evaluation of prognostic factors in endometrial tumors].

There were analysed prognostic factors in endometrial carcinomas. To essential prognostic factors were counted; proportion of cells in S-phase, DNA ploidy, proportion of Ki-67 positive cells and vascular invasion by cancer cells. All above-mentioned factors were not correlated to either the stage or the histopathologic subtype of the cancers. Application of proliferating markers may be useful for selecting a subgroup of patients for adjuvant therapy.

Adult↗
PubMed1997

[Ectopic cervical pregnancy--conservative treatment with methotrexate].

Ectopic pregnancy regardless its localization always remains difficult diagnostic and therapeutical problem. Special attention is paid to cervical pregnancy because of its rare incidence and poor prognosis (even in this day and age maternal death rate is 40-45%). In this paper there has been reported the case of 38 years old patient, admitted to Dept of Gynecological Oncology with suspicion of alive cervical pregnancy. Diagnostic procedures have been described as well as positive results of conservative treatment with Methotrexate (under careful monitoring--ultrasound, beta hCG levels). Also there has been presented detailed description of endocervix curettage after fetal death confirmed ultrasonographically. Current conservative therapy methods for cervical pregnancy have been discussed.

Adult↗
PubMed1997

[Primary melanoma of the uterine cervix in a 19 year old woman--case report].

UNLABELLED: Only 5% of all melanocytic malignancies in women originate in the genitalia, the most unusual of which is melanoma involving the uterine cervix. CASE: a 19 year old woman, presented with abnormal genital bleeding. In our hospital the clinical examination showed an exophytic bleeding tumor growing in the cervix uteri. A first vaginal biopsy showed a sarcoma colli uteri. A new biopsy confirmed the clinical diagnosis of malignant melanoma. Subsequently a Wertheim-Meigs hysterectomy with bilateral adnexectomy and removal of upper half of the vagina and regional lymphadenectomy was performed. Next the patient received several courses of chemotherapy (DTIC).

Adult↗
PubMed1997

[Doppler ultrasonography for detection of pathologic changes in uterine body endometrium].

This study was conducted to evaluate the role of transvaginal color Doppler ultrasonography in detecting endometrial pathology in postmenopausal women A group of 140 women with abnormal postmenopausal bleeding was examined by TVS with color Doppler before D & C. Histopathologic and blood flow characteristics were evaluated. Abnormal blood flow characteristics were evaluated. Abnormal blood flow within endometrium was visualized in 78% cases of cancer. RI and PI values were lowered in hyperplasia and significantly in cancer groups. Transvaginal color Doppler ultrasonography can depict endometrial pathology, even in asymptomatic women.

Aged↗