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Biomedical subjects

M Sprössig

Publications and source records attributed to M Sprössig.

At least 37 records · Page 2Linked to original sources
PubMed1982

[Contribution to the true non-African Burkitt's lymphoma (author's transl)].

It is reported on a 6-year-old Middle European boy suffering from a nasopharynx tumor with involvement of a submandibular lymph node. The tumor was classified by morphologic, serologic and virologic data as a true or Epstein-Barr virus-associated Burkitt's lymphoma, resp., which has been, up to now, seldom seen outside Africa. The patient died 3 months after diagnosis. Autopsy has shown tumor involvement of nasopharynx and soft tissues in the left neck region, several lymph nodes, kidneys, liver, spleen, and bone marrow. Cytologic, light and electron microscopic data are reported and the structural change of the tumor is referred to.

Autopsy↗
PubMed1982

[Bacteriological examination of subgingival flora in patients with marginal periodontitis].

Before and 4 weeks after a single course of local treatment, the composition of subgingival microflora of 15 patients with marginal periodontitis was determined by cultural and microscopical examination. A significant reduction in the number of gram-negative microorganisms could be achieved by this treatment. Anaerobic sporeless rods like Bacteroides and Fusobacteria but also Spirochetes, Eikenella and Capnocytophaga were involved in the inflammation. The number of Actinomyces in the subgingival flora was also significantly reduced by therapy. After treatment the number of gram-positive bacteria like Streptococci and Corynebacteria was significantly increased. The reduction in the number of these gram-negative bacteria in line with the reduction of periodontal inflammation is a criterion of their significance in the etiology of marginal periodontitis.

Actinomycetales↗
PubMed1981

[Comparison of the in vitro activities of ammonium humate and of enzymically oxidized chlorogenic and caffeic acids against type 1 and type 2 human herpes virus (author's transl)].

Ammonium humate (prepared from marsh water humic acids) as well as enzymically oxidized diphenolic compounds such as the oxidation products of chlorogenic and caffeic acids have strong antiviral activities in vitro. The effective concentrations lie considerably outside the cytotoxic range of the compounds mentioned.

Adsorption↗
PubMed1980

[Demonstration of Epstein-Barr virus genome in european Burkitt lymphoma (author's transl)].

A 6-year-old European boy with a tumour in the head and neck region died 86 days after the first occurrence of symptoms despite chemo- and radiotherapy. An epipharyngeal biopsy at the beginning of the disease and a cytological investigation of biopsy and autopsy material showed non-Hodgkin lymphoma with a high degree of malignancy of the Burkitt type. High antibody titres against Epstein-Barr virus (EBV)-specific virus capsid antigen, nuclear antigen and the R component of the EBV-early-antigen-complex as well as the approximately 27 EBV genome equivalents per tumour cell, demonstrated by DNA-DNA hybridisation, characterised the tumour as true Burkitt lymphoma which is observed extremely seldom outside Africa.

Antigens, Viral↗
PubMed1980

Diagnosis of Herpesvirus hominis infections with the use of Ehrlich mouse ascites tumour cells.

A new technique for the detection of Herpesvirus hominis (HVH) in materials from patients, based on mixed haemadsorption with Ehrlich mouse ascites tumour cells, was proposed. Investigations on 100 pustule punctates and smears from 75 patients with typical clinical signs of various primary and recurrent infections yielded positive results in 80% of cases. This was in good accordance with HVH isolations in various cell cultures. The new technique can, therefore, be recommended for routine diagnosis. Its main advantage is the rapid virus diagnosis.

Animals↗
PubMed1979

Human cytomegalovirus induced changes in rabbit cells. Brief report.

After infection with human cytomegalovirus, rabbit lung fibroblasts showed, during the four week period of the experiment, cytopathic changes and virus-specific antigens demonstrable by fluorescent antibody. Infectious virus could be recovered from the infected cells by co-cultivation with human lung fibroblasts.

Animals↗
PubMed1979

Determination of antibodies against cytomegalovirus-induced early antigens by using rabbit lung fibroblasts. Brief report.

The determination of antibodies against cytomegalovirus-induced antigens in 30 patients with acute cytomegalovirus infection and in 100 healthy control persons, using early antigen preparations of human lung fibroblasts and of rabbit lung fibroblasts, yielded results showing a high degree of agreement. For such investigations, however, the use of rabbit cells proved to be of advantage because the preparation of the early antigens can be carried out without inhibition of the DNA synthesis of the host cells.

Adult↗
PubMed1978

[Etiology of chronic recurring aphthous stomatitis].

46 patients with chronic recurrent aphthae of the oral mucosa were subjected to clinical, virological and serologic examinations to evidence the assumed virus aetiology of this clinical picture. A cytopathogenic organism (classified as herpes virus hominis) could be cultivated from the aphtha sample from only one patient.

Antibodies, Viral↗
PubMed1977

[Virus-induced autoimmunity phenomena in experimental animals (author's transl)].

QUESTION: It may happen that the virus infection causes changes of the immunogenicity of the host cells that may theoretically result in disturbances of the "self-recognition" of the organism. In this paper we report on the immunization of rabbits by autologous testes cells infected with herpes-virus hominis (HVH) types 1 and 2. MATERIAL AND METHODS: In 20 rabbits the excision of one testis was carried out. The testes cells were cultivated in L 15-medium (Leibowitz) and lactalbumine hydrolysate supplemented with 12% autologous serum. The virus strains herpesvirus hominis type 1 (Kupka) and type 2 (US) were passaged three times on autologous testes cells, in order to remove host-foreign proteins out of the envelope. Thereafter, the monolayer cultures grown in Demeter flasks were infected with 15 ml virus suspension containing 10(4) to 10(5) TCID50. For the immunization three antigen preparations were applied: a) Supernatant of virus-infected cell cultures, b) Pellets of the virus-infected cell cultures, c) Pellets of the non-infected cell cultures. Two times at an interval of 3 weeks the animals were immunized by intramuscular and intraperitoneal injections with prepared antigens. Altogether, 20 animals were immunized: 15 rabbits with virus antigen, 5 control animals with non-infected autologous cells. After 28 days, 13 rabbits were bled, the second testis, liver, spleen, and kidneys were taken out. From 7 rabbits, only the second testis was taken out. These animals got a further injection on the 40th day post infectionem, and on the 50th day, they were bled. From the tissue samples, frozen sections were cut, fixed with acetone, and treated with fluorescein isothiocyanate-labelled antirabbit globulin, in order to elicite the tissue-bound antibodies. For histological studies, paraffin sections were stained with hematoxylin and eosin, and with azan, besides the PAS reaction was carried out. The determination of humoral antibodies was done by means of indirect-immunofluorescence antibody technique (IFAT). As antigen, autologous cells grown on slides were used; a part of them was infected with HVH types 1 or 2, while another part was not infected. RESULTS: After immunization with autologous testes cells which were infected with HVH types 1 or 2, histologically homogeneous eosinophilic PAS-positive substances were detected in 14 out of 15 rabbits, mainly in the interstitium of the testes (fig. 2). These substances corresponded to globulin deposits that could be demonstrated by the reaction of labelled antirabbit globulin (fig. 1). The testes sections of thoses 5 rabbits that were injected with noninfected autologous cells, did not react. On the 28th day post infectionem, the antibody levels against virus antigen were found at the degree of 1:32 to 1:1024. Only after the removal of the second testis, there occurred antibodies against autologous testes cells. The immunization conditions exclude the formation of isoantibodies...

Animals↗
PubMed1977

[In vitro studies of the antiviral activity of ammonium humate against herpes simplex virus type 1 and type 2 (author's transl)].

Ammonium humate, isolated from peat water, is a higher molecular polyphenolic compound with a strong antiviral activity against herpes simplex virus type 1 and type 2. Three cell lines were chosen for examining the cytotoxicity of ammonium humate: rabbit kidney primary cells, HEp-2- and FL-cells. As the light microscopic examination of both, the monolayers and the stained cover-glass cultures, revealed, concentrations of up to 5 microgram/ml ammonium humate can be used without hesitation for rabbit kidney primary cells (Fig. 1, 2) 200 microgram/ml for HEp-2-cells (Fig. 3, 4, 5), and 2000 microgram/ml for FL-cells. Fresh prepared rabbit kidney primary cells and suspended FL-cells (Tab. 1) are most sensitive against ammonium humate than monolayers. A remarcable plaque inhibition effect on the multiplication of herpes simplex virus type 1 and type 2 has been observed in all cell systems at concentrations of 0.5-20 microgram/ml ammonium humate (Fig. 6). The inhibition of virus multiplication at concentrations of greater than 10 microgram/ml ammonium humate was independent of the incubation temperature (Fig. 8). The adsorption of the virus to the cell surface has been found to be the most humate-sensitive phase of the herpesvirus multiplication cycle (Fig. 7, 9, 10). A prophylactic effect in vitro could be observed at concentrations greater than 10 microgram/ml ammonium humate (Fig. 11, 12).

Adsorption↗