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Biomedical subjects

M Sprössig

Publications and source records attributed to M Sprössig.

At least 55 records · Page 3Linked to original sources
PubMed1977

[Urinary tract infections in surgical gynecology with special reference to the urine withdrawal technic].

In a direct comparison series between 400 midstream urinesamples and 400 suprapubic aspiration specimens, but as well in an indirect comparison series of the same number of catheter urine-samples, bacteriological examinations were executed by means of the culture method before and after gynaecological operations. 19,5% of the suprapubic aspirates, 20,5% of the catheter specimens, and 25,5% of the midstreamsamples showed a significant bacteriuria. The number of false positive findings was in regard of the midstream urinesamples more than a quarter of the total infection rate. In midstreamsamples, mixed infections were 3,4 times more frequent than in catheter specimens. After a discussion of the results under special consideration of the high number of findings needing control in connection with operative problems, a valuation of the obtaining of urine in the field of operative gynaecology is demonstrated.

Bacteriuria↗
PubMed1976

[Herpesvirus-induced autoantibodies in animals (author's transl)].

After immunization with autologous testicle cells which were infected with herpes virus hominis type 1 or 2, histologically homogeneous, eosinophilie PAS-positive substances could be detected in five out of six rabbits, mainly within the interstitium. These substances have been found by immunofluorescence microscopic techniques to be antibodies against testicle tissue.

Animals↗
PubMed1976

[The coxsackievirus-infected mouse: an animal model for the study of virus myocarditis].

Basing on the experimental infection of adult mice with coxsackie virus A9 reported by Lerner in 1965 and the successful cultivation of the virus from the hearts of experimental animals, a model for studying virus myocarditis is proposed. By a combination of factors favouring the infection (age of the animals above 1 year, physical load prior to infection by repeated swimming) a high virus re-isolation rate was achieved. Cultivation of the virus from the mouse heart was possible on the 3rd day p.i. in 81%, on the 6th day in 71%, on the 9th day in 31%, and on the 12th day in 12% of the cases. In 66% of the animals towards the end of the period of observation a significant increase in few cases is the virus isolated on the 3rd and 6th day p.i. also from the skeletal muscles. The model can be used both for pathogenetic studies on myocardites elicited by cardiotropic viruses and for in vivo testing of antiviral substances.

Animals↗
PubMed1976

[Cold sterilization of serums with peracetic acid].

On the basis of broad antimicrobic effect including also bacterial spores, mycoplasmas and viruses, the peracetic acid suits to sterilize in cold. By means of the final 0,1 to 0.02% concentration of the peracetic acid acting for 30 min. up to 5 days, it succeeded in sterilizing the serum used to prepare culture medium for bacteria, mycoplasmas and tissue cultures. Growth controls shown at least as good results as were those in using filtered sera. Chemical sterilization by means of the peracetic acid leads to significant diminution of neutralization and complementfixation antibodies in serum.

Acetates↗
PubMed1976

[Serum sterilization in the cold with peracetic acid].

On the basis of broad antimicrobic effect including also bacterial spores, mycoplasmas and viruses, the peracetic acid suits to sterilize in cold. By means of the final 0,1 to 0.02% concentration of the peracetic acid acting for 30 min. up to 5 days, it succeeded in sterilizing the serum used to prepare culture medium for bacteria, mycoplasmas and tissue cultures. Growth controls shown at least as good results as were those in using filtered sera. Chemical sterilization by means of the peracetic acid leads to significant diminution of neutralization and complementfixation antibodies in serum.

Acetates↗
PubMed1975

Suitability of peracetic acid for sterilization of media for mycoplasma cultures.

The utility of peracetic acid for sterilization of serum and yeast extract additions to mycoplasma medium was studied by culturing six Mycoplasma species. Culture media containing additions that had been sterilized with peracetic acid proved to be as good as filtered components. The use of 0.05 to 0.1% peracetic acid is recommended to sterilize the serum and yeast extract additions since savings in time and equipment can be accomplished.

Acetates↗
PubMed1975

[Beta-propiolactone for cold sterilization of heart valve transplants].

Biologic cardiac valve substitutes were sterilized at room temperature using beta-propiolactone with a 0.5 vol.% solution within 120 minutes, with a 1.0 vol.% solution within 90 minutes, and with a 1.5 resp. 2.0 vol.% solution within 60 minutes. The physico-chemic properties of the graft can be influenced by appropriately chosen concentration and sterilizing period. Enlarged procedures for decontamination are not necessary because of the rapid dissolution of aqueous beta-propiolactone solutions.

Animals↗
PubMed1975

[Combined cleaning and cold sterilization procedure for cleaning rooms in virological establishments].

It is reported of a new technique for the cleansing and cold sterilization of glass-ware which is particularly suited for virological laboratories and other laboratories working at cell and tissue cultures. The combined use of an alkaline cleansing agent and sterilization with Wofasteril, a peracetic acid preparation, permits to accelerate considerably the return of culture flasks for re-use.

Acetates↗