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Biomedical subjects

M T Hull

Publications and source records attributed to M T Hull.

At least 37 records · Page 2Linked to original sources
PubMed1987

Pneumocystis carinii pneumonia therapy with 9-deazainosine in rats.

An inosine analog, 9-deazainosine, has previously been demonstrated to inhibit Pneumocystis carinii in culture with WI-38 cells. The present study shows that it is also effective against Pneumocystis carinii in immunosuppressed Sprague-Dawley rats with Pneumocystis carinii pneumonia. After 8 wk of immunosuppression, rats that developed severe Pneumocystis carinii pneumonia were treated with either 9-deazainosine or served as controls. After 15 days of therapy, animals were sacrificed and severity of infection determined by morphologic examination of lungs for numbers of Pneumocystis carinii. Treated animals had greatly reduced numbers of Pneumocystis carinii trophozoites and cysts, compared with controls. This drug shows promise for therapy of Pneumocystis carinii pneumonia and should be studied further.

Animals↗
PubMed1987

Papillomavirus-induced changes in the topography of the epidermal-dermal junction: a scanning electron microscopic study of basal lamina.

Papillomavirus infects human epidermal cells and causes verruca vulgaris, which is characterized by altered epidermal growth rates and differentiation patterns. There is also a prominent induced dermal proliferative response. Ten formalin-fixed skin specimens from 10 patients, each with histologically characteristic verruca vulgaris, were excised from paraffin blocks, deparaffinized in xylene, and rehydrated in graded ethanol solutions. Hydrated specimens were incubated in 90% formic acid at 45 degrees C for 18 h. Using a dissecting microscope the epidermis was gently separated from the dermis, which then was fixed in 3% glutaraldehyde and processed for scanning electron microscopy. Scanning electron microscopy showed a well-preserved epidermal-dermal junction (EDJ) covered by basal lamina. In comparison with normal EDJ, the EDJ of verruca vulgaris showed markedly elongated dermal papillae. Some papillae were broad and thin, and others were broad and thick. Irregularly shaped and branching secondary papillae were common. Also, there were changes in the basal lamina: the normal corrugation pattern was replaced by relatively smooth undulations or coarse, vertically oriented ridges on papillae. Papillary tips were often smooth. This study shows that there were prominent alterations in the topography of the EDJ and basal lamina in verruca vulgaris. This technique should be useful in evaluating epithelial-connective tissue morphologic interactions in formalin fixed archival tissue in other diseases characterized by alterations in epidermal growth rates and differentiation patterns.

Adolescent↗
PubMed1987

Mucinous breast carcinomas with abundant intracytoplasmic mucin and neuroendocrine features: light microscopic, immunohistochemical, and ultrastructural study.

Eight mucinous carcinomas of the breast were studied by light microscopy and immunohistochemistry; one was studied by electron microscopy. All 8 cases had abundant, relatively clear cytoplasm that contained mucin. Cells were argyrophil positive and argentaffin negative. Eight cases were positive for neuron specific enolase (NSE), 5 cases for serotonin, 1 case for serotonin and somatostatin and 2 cases for serotonin, somatostatin, and gastrin. None had clinical evidence of abnormal neuroendocrine function. Three patients had axillary lymph node metastases. Only 1 of 5 patients in whom there was clinical followup died of her disease. Electron microscopy of one case showed abundant intracytoplasmic and extracellular mucin, round and pleomorphic dense-core granules, numerous cell processes, and aggregates of intermediate filaments. These cases expand the histologic spectrum of breast carcinomas which may show neuroendocrine differentiation.

Adenocarcinoma, Mucinous↗
PubMed1987

Glycogen-rich clear cell carcinoma of the urethra: an ultrastructural study.

A 49-year-old black woman developed a urethral glycogen-rich clear cell carcinoma. She was treated with anterior pelvic exenteration. The resected lymph nodes, vagina, uterine cervix, endometrium, ovaries, and urinary bladder were free of neoplasm. Histologically the neoplasm consisted of clear cells growing in sheets and occasional papillary structures. In some areas, hobnail cells were present. Ultrastructurally, the cells had apical caps, short microvilli, and complex cell bases, and contained abundant glycogen. These features were identified in one, but not the other of two previously reported cases. Because glycogen-rich clear cell carcinomas of the lower urinary tract do not resemble ultrastructurally mesonephric remnants or carcinomas known to arise from them, these glycogen-rich clear cell carcinomas should not be called "mesonephromas" as has been the practice.

Adenocarcinoma↗
PubMed1986

Villous adenoma of the urachus with mucusuria: a light and electron microscopic study.

After 2 years of mucusuria a villous adenoma of the urachus was resected from a woman by partial cystectomy and excision of the entire urachus. Convalescence was uneventful and the patient was well 12 months later. Urachal adenomas are rare, this being the sixteenth case reported. Generally, they are multilocular cystic tumors lined by columnar epithelium with a population of mucous goblet cells. Often, there is a striking resemblance to gut epithelium. Ultrastructurally, by transmission and scanning electron microscopy, the tissue resembles gut mucosa. Urinary mucus is a common and relatively specific symptom for adenomas of the lower urachus, occurring in 7 of 11 cases. Resection alone is effective therapy but care must be exercised to avoid spilling adenoma cells in the peritoneum.

Adenoma↗
PubMed1986

Glycogen-rich clear cell carcinomas of the breast. A clinicopathologic and ultrastructural study.

Ten cases of glycogen-rich clear cell carcinoma of the breast are described. Only two previous case reports have been published. These neoplasms are composed of clear cells with abundant glycogen. Ultrastructurally, two cases showed large quantities of non-membrane-bound glycogen and numerous empty glycogen lakes. neoplastic cells formed tight junctions, immature desmosomes, and occasionally had short microvilli. In nine cases the glycogen-rich carcinoma grew in a solid pattern only, while one case had both solid and papillary patterns. One case was associated with a signet-ring cell carcinoma. Seven of nine patients who underwent axillary dissections had nodal metastases. Five patients died with residual disease, and one is currently alive with local skin recurrence. These data suggest that glycogen-rich clear cell carcinoma is associated with frequent lymph node metastases and mortality.

Adenocarcinoma↗
PubMed1986

Ultrastructure of abnormal collagen in human tumors.

Abnormal collagen fibrils were identified by transmission electron microscopy in 22 human tumors of differing histogenesis. They were found in a review of 1400 electron microscopy cases. Abnormal collagen fibrils, described by others as amianthoid fibers, composite collagen, collagen flowers and intrafibrillar collagen dysplasia, have been found only rarely in human tumors but commonly in certain connective tissue diseases such as pseudoxanthoma elasticum, Ehlers-Danlos syndromes, Marfan's syndrome, osteoarthritic cartilage, and emphysematous lung among others. Abnormalities in the cases described here included thickened fibrils, fibrillar degeneration of fibrils and irregular external contours. Proposed mechanisms for their formation have included degeneration possibly due to hypoxia or collagenase activity, abnormal collagen biosynthesis, and abnormal tissue levels of glycosaminoglycans. The finding of abnormal collagen fibrils in these 22 human tumors shows that their occurrence is more common than is indicated by previous published reports. Most of the tumors containing abnormal collagen fibrils were mesenchymal or soft tissue tumors. Four neuroendocrine neoplasms had abnormal collagen fibrils.

Adolescent↗
PubMed1986

Villous adenoma of the uterine cervix associated with invasive adenocarcinoma: a histologic, ultrastructural, and immunohistochemical study.

An unusual neoplasm of the uterine cervix had the features of a villous adenoma with an adjacent adenocarcinoma. Histologic and electron microscopic features of the lesion are described, as well as immunohistochemical staining of the lesion for carcinoembryonic antigen. Electron microscopic evidence of intestinal differentiation in the villous adenoma suggests a rarely recognized potential for intestinal metaplasia in the uterine cervix. The association of villous adenoma with cervical adenocarcinoma underscores the malignant potential and the need for complete excision of this rare cervical lesion.

Adenocarcinoma↗
PubMed1986

Basal lamina at the epithelial-connective tissue junction in the rat forestomach, esophagus, tongue and palate: scanning electron microscopic study.

The squamous epithelium lining the rat foregut was removed by incubating fresh, unfixed specimens in 2N sodium bromide. The surface morphology of the exposed subepithelial basal lamina was examined by scanning electron microscopy. Areas examined included hard and soft palates, oropharynx, tongue, esophagus, and forestomach. The basal lamina was continuous were not present at all sites. The saucer-like defects of lymphocyte migration that are present in the basal lamina beneath the squamous epithelium of the skin were not observed in rat foregut. The epithelial-connective tissue interface of the rat esophagus does not have the coiled and branched papillae seen in esophagi of adult humans. The three dimensional shapes of the connective tissue cores of the various lingual papillae are well-demonstrated by this technique and are distinct. The basal lamina of the hard and soft palates are also distinct.

Animals↗
PubMed1985

Laryngeal blastoma. A light and electron microscopic study of a novel entity analogous to pulmonary blastoma.

A neoplasm morphologically analogous to pulmonary blastoma, but arising in the pyriform fossa of the larynx, is described and illustrated. The patient, a 65-year-old white man, was treated with laryngectomy and is alive and free of disease 13 months later. Grossly polypoid on a broad stalk, the neoplasm consisted of a mixture of blastematous, mesenchymal, and epithelial elements. Ultrastructural examination showed that the spindle and stellate cells were truly mesenchymal. The name "laryngeal blastoma" is proposed for this novel entity.

Aged↗
PubMed1984

Migration of lymphocytes through the cutaneous basal lamina in normal skin: an ultrastructural study.

Incubation of skin in 2 N sodium bromide allows separation of dermal and epidermal layers leaving an intact basal lamina covering the dermal portion. Examination of the surface of the dermis by SEM shows cells migrating through the basal lamina. By scanning and transmission electron microscopy, these cells have the characteristics of lymphocytes. The migrating lymphocytes produce a sequence of basal lamina deformations including dome formation, effacement of corrugations, and central fenestrations with hole formation allowing lymphocyte passage. Following passage there is reestablishment of a relatively smooth basal lamina in the crater base, effacement of the crater rim, and finally reformation of basal lamina corrugations. This deformability of the basal lamina supports the hypothesis that basal lamina is thixotropic. This study is the first demonstration in three dimensions of lymphocyte traffic across the basal lamina, an important component of skin-associated lymphoid tissue (SALT).

Adult↗
PubMed1984

Malignant sex cord-stromal tumor of testis.

We report a case of sex cord-stromal tumor in the testis, which metastasized to the retroperitoneal space, resulting in death 13 months after orchiectomy. The primary lesion consisted of granulosa cells, theca cells, Sertoli cells and undifferentiated gonadal stroma. Biopsy of the retroperitoneal metastasis showed predominance of the Sertoli cell component.

Adult↗
PubMed1984

Morphologic features of renal oncocytoma: a light and electron microscopic study.

Five renal oncocytomas were studied by light and electron microscopy. Five glutaraldehyde-fixed blocks from each tumor were examined. Scarce short, blunt microvilli, plasmalemmal interdigitations, basement membrane protuberances, and round or oval mitochondrial profiles were found, suggesting that these neoplasms arise from the epithelium of the distal tubule. Morphologic criteria for the diagnosis of renal oncocytoma, including ultrastructural demonstration of cytoplasmic filling by mitochondria, are presented.

Adenoma↗
PubMed1984

Villous papilloma of the gallbladder in association with leukodystrophy.

A 2-year-old girl with metachromatic leukodystrophy had an unusual papilloma of the gallbladder. The association of metachromatic leukodystrophy and gallbladder disease in children is recognized. The striking anemone-like configuration of this tumor is remarkably unlike those of other papillomas described in the literature.

Child, Preschool↗
PubMed1984

Extrapulmonary oat-cell carcinoma of the tongue: an electron-microscopic study.

An extrapulmonary oat-cell carcinoma arose in the tongue of a 62-year-old man who had a long history of cigarette smoking and ethanol ingestion. At presentation, he had the primary tongue lesion and cervical lymph-node metastases only, and search for a pulmonary primary was negative. Ten months later he died with metastases to liver, cerebrum, ribs, vertebrae, calvarium, and clavicle. Electron microscopy demonstrated cells with cytoplasmic processes and many dense-core neurosecretory granules that had a tendency to concentrate in the processes. Cells were linked by desmosomes. Electron microscopy is necessary for definitive diagnosis of extrapulmonary oat-cell carcinomas.

Carcinoma, Small Cell↗
PubMed1984

Giant melanosomes in the dysplastic nevus syndrome. Electron microscopic observations.

Multiple pigmented lesions including benign lentigines, typical nevi, and dysplastic nevi from 3 patients with the dysplastic nevus syndrome contained giant melanosomes (GM). Ultrastructurally the GM were composed of microvesicles, but lacked limiting membrane and filamentous internal structure. As such, they resembled the GM found in 4 other patients with dysplastic nevus syndrome, leopard syndrome and ocular albinism, but differed from those found in the café-au-lait spots of neurofibromatosis, nevus spilus, vitiliginous achromia, and certain animals.

Humans↗
PubMed1984

Scanning electron microscopic study of the epithelial-mesenchymal junction of the esophagus.

This is a study of the appearance by scanning electron microscopy of the stromal papillae and basal lamina at the epithelial-mesenchymal junction of the human esophagus. After incubation in 2N sodium bromide at 60 degrees C. for 1 hour, the squamous epithelium was removed gently under the dissecting microscope from esophageal specimens from six normal human esophagi. The remaining stromal surface covered by an intact smooth basal lamina was examined "en face" by scanning electron microscopy. Transmission electron microscopy confirmed that the separation following NaBr was between the basal cell plasma membranes and the basal lamina, and that the basal lamina and subbasal connective tissue were well-preserved and attached normally to each other. Complex topographical patterns consisting of various numbers of simple and branched papillae and blade-like stromal folds were observed. Papillae had helical contours because of the spiral capillaries within them. Subepithelial capillaries were also visible as interconnecting linear ridges in the interpapillary zones. There was great variation in papillary length and patterns between individuals, but there was no difference in patterns or papillary length in sections taken from lower, middle, and upper portions of the esophagus in a given patient. These findings suggest that comparisons of absolute length of stromal papillae in different individuals should not be interpreted as a pathologic change. The results reveal an unexpected complexity in this epithelial-stromal interface.

Adult↗