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Biomedical subjects

M Tsukuda

Publications and source records attributed to M Tsukuda.

At least 109 records · Page 6Linked to original sources
PubMed1993

Production of granulocyte colony-stimulating factor by head and neck carcinomas.

Detectable levels of G-CSF by enzyme-linked immunosorbent assay (ELISA) were found in sera of 4 out of 15 patients with head and neck carcinomas. Also cells prepared from the tumors of these 4 patients secreted G-CSF. The supernatants of cells derived from all 15 patients did not contain granulocyte-monocyte CSF, monocyte CSF, tumor necrosis factor-alpha, transforming growth factor-beta 1, epidermal growth factor, interleukin (IL)-1 beta and IL-6. These findings suggest that leukocytosis in patients with carcinomas might be due to the production of G-CSF by tumor cells.

Cell-Free System↗
PubMed1993

A tumor cell line producing granulocyte colony-stimulating factor and an immune suppressive factor.

From a patient, both a cell line incapable of secreting granulocyte colony-stimulating factor (G-CSF) (TC873) and a cell line capable of secreting G-CSF (TCM902) were established. The effector cells induced, with TC873 cells showed a high lytic capacity against two types of tumor cells. The effector cells induced by TCM902 cells did not show such capacity. Furthermore, the TCM902 cells excreted a factor suppressing the proliferation of lymphokine activated killer (LAK) cells and the autologous tumor cell lysis of tumor associated lymphocytes. This factor probably is TFG-beta 1.

Cell-Free System↗
PubMed1993

The sensitivity of head and neck squamous cell carcinomas to tumor necrosis factor-alpha.

Sensitivities to recombinant human tumor necrosis factor-alpha (TNF-alpha) and chemotherapeutic agents (cisplatin, peplomycin, methotrexate) were evaluated in 20 tumor cells of head and neck squamous cell carcinomas, using a dye uptake method. Also, numbers of TNF receptors of these tumor cells were measured by Scatchard plot analysis. There was no relationship between the number of TNF-alpha receptors and the sensitivity to TNF-alpha. Furthermore, there was no correlation between the sensitivity to TNF-alpha and that to chemotherapeutic drugs, nor between the sensitivity to TNF-alpha and the clinical response to chemotherapy including of cisplatin and peplomycin. The sensitivity to TNF-alpha was higher in poorly differentiated carcinomas than in well differentiated ones.

Antineoplastic Agents↗
PubMed1993

Induction of cytolytic activity of lymphocytes from carcinomatous pleural effusion by IL-2 and autologous tumor cells.

We established a cell line (STKM-1) from tumor cells obtained from carcinomatous pleural effusion of a gastric cancer patient. The lymphocytes separated from her peripheral blood or pleural effusion were cryopreserved and immunological experiments were performed after the establishment of the cell line. They were treated with IL-2 or with both IL-2 and mitomycin C (MMC)-treated autologous STKM-1 cells. The cytolytic activity against STKM-1 cells was elevated in lymphocytes cultured with IL-2, and was more prominently augmented in lymphocytes cultured with both IL-2 and MMC-treated STKM-1 cells. The elevation in cytolytic activity was more marked with pleural effusion lymphocytes than with the peripheral blood lymphocytes. The results suggest that the lymphocytes obtained from the pleural effusion would be an excellent source for adoptive immunotherapy.

Adenocarcinoma, Scirrhous↗
PubMed1993

The hazards of alkaline disk batteries as foreign bodies in the nose.

This paper reports a case of intranasal chemical burn and septal perforation caused by an alkaline disk battery accidentally entrapped in the nasal cavity of a 3-year-old girl. The battery in the nasal cavity was removed after about 44 hours, but nasal septal perforation without nasal deformity occurred about 3 weeks after accident.

Burns, Chemical↗
PubMed1993

Extramedullary plasmacytoma of the larynx.

A case of IgD myeloma in a 54-year-old male with a long-standing history of extramedullary plasmacytoma involving the larynx is reported. The patient was treated with radiation therapy and laryngectomy. Twelve years later, he complained of nasal bleeding. On examination he was found to have large masses in the left nasal cavity and in the left supraclavicular region. Histological examination of both lesions showed plasmacytoma. Serum immunoglobulin studies revealed an IgD monoclonal spike of the lambda type. Bence-Jones protein was present. Using the immunoperoxidase staining technique, cytoplasmic monoclonal IgD was detected.

Bone Neoplasms↗
PubMed1993

Tumours metastasizing to the head and neck--a report of seven cases.

Metastatic tumours involving the head and neck region are rare. Over the past 18 years, seven such cases were treated at our clinic. Of those, four were in one of the paranasal sinuses, three had arisen from a primary hepatocellular carcinoma and one from an osteogenic fibrosarcoma of the leg. In the remaining three cases, metastases to the larynx, the tonsil, and the parotid gland arose from a primary renal cell carcinoma, a thyroid carcinoma, and a breast carcinoma, respectively. In metastatic tumours, the primary site can often be identified by the histopathological features. Accordingly, when malignant head and neck tumours are suspected of being metastatic in character, it is important to search carefully for the primary site.

Adult↗
PubMed1993

Head and neck carcinomas in patients under the age of forty years.

From 1973 to 1988, 1,104 patients with head and neck carcinomas (excluding thyroid carcinoma) were admitted to the Yokohama City University Hospital, and among them, 48 (4.3%) were under the age of 40 years. We made a retrospective survey of these young patients. There were 36 males and 12 females. An increasing ratio of male to female was seen with increasing age. The primary site was most frequently the nasopharynx (29 cases), followed by the maxillary sinus (9 cases), and the tongue (4 cases). Squamous cell carcinoma in these young patients characteristically showed a low grade of differentiation.

Adolescent↗
PubMed1993

[Fine needle aspiration cytology under ultrasonographic imaging].

Although fine needle aspiration cytology (FNA) is widely used in the otolaryngology, few hospitals perform it in conjunction with ultrasonographic imaging. Despite the benefits of ultrasonographic imaging-assisted FNA, it is not widely utilized due to the prevalent misperception that the procedure is exceedingly complicated. In an effort to refine FNA procedures under ultrasonographic imaging (focusing on safety and simplification), we conducted a study on outpatients with neck tumors. We developed the following guidelines. 1. The aspiration was conducted free-hand without the use of mechanical devices such as special probes and/or aspiration adaptors. 2. Positioning of the probe was adjusted so that the puncture site was centered in the image. 3. When the tumor was located near the carotid artery or jugular vein, the probe was adjusted so that both the tumor and the blood vessel could be observed in the same image. 4. The needle was introduced at the center of the probe from the sliced direction. The needle injection route was then adjusted in line with phase crossing at right angles with the scanning field of the probe. 5. The depth of the puncture was adjusted in accordance with the angles between the probe and the needle. From June 1985 to December 1990, FNA under ultrasonographic imaging was conducted on 149 patients. It was possible to compare both cytological and histological studies. According to site, lymph nodes ranked highest in 52 cases, followed by parotid glands (45) and thyroid glands (27). A 5 or 7.5 MHz probe was used with 21- or 22-gauge injection needles.(ABSTRACT TRUNCATED AT 250 WORDS)

Biopsy, Needle↗
PubMed1993

[Angiogenesis in head and neck tumor].

To clarify the correlation between tumor angiogenesis and tumor growth in head and neck carcinomas, we investigated the number of microvessels, using immunohistochemical factor VIII. No correlations among this number, differences in the primary lesion, histological differentiation and T classification were detected. The incidence of neck lymph node metastases increased as microvessel numbers increased in tumor sites. The number of microvessels increased as N and Stage classification progressed. The number of microvessels in CR cases after induction chemotherapy were increased. The numbers of microvessels in patients without recurrence were apparently greater than those in patients with recurrence. The results of this study suggest that the number of microvessels in a primary tumor correlates with the metastatic ability of the tumor.

Adult↗
PubMed1993

[Comparison of granisetron alone and granisetron plus hydroxyzine hydrochloride for the prophylactic treatment of emesis induced by cisplatin-containing chemotherapy].

In this study, the utility and safety of granisetron alone (Group G) and granisetron plus hydroxyzine hydrochloride (Group G/H) for nausea and vomiting were evaluated in patients with head and neck cancer treated by cisplatin-containing chemotherapy. There was no statistically significant difference between the two groups in the severity of nausea or frequency of vomiting, although all patients in the G/H group showed complete response (no nausea or vomiting) from the fifth day after cisplatin administration. The clinical utility rate was higher in Group G/H than in Group G. The only side effect observed was headache in one patient from Group G. No drug-related abnormality in laboratory tests was observed. These results demonstrate that the antiemetic efficacy of granisetron can be augmented by the addition of hydroxyzine hydrochloride, providing superior control of emesis induced by cisplatin-containing chemotherapy.

Adult↗
PubMed1992

[Sensitivity test to chemotherapeutic agents and cytotoxicic test against immuno-effecter cells of established malignant fibrous histiocytoma cell line].

UNLABELLED: A cell line was established from a patient with malignant fibrous histiocytoma, which had originated in the maxillary sinus. Using this cell line, sensitivity to chemotherapeutic agents and cytotoxicity against various immunoeffecter cells were tested. RESULTS: This MFH cell line was sensitive in some degree to adriamycin, 5-fluorouracil, cisplatin, peplomycin, and methotrexate at high doses, but insensitive to mitomycin-C, vincristine and cyclophosphamide. Furthermore, this cell line showed no sensitivity to cytolytic cytokines, such as tumor necrosis factor-alpha and interferon-gamma. Lymphokine activated killer (LAK) cells and lymphokine activated tumor infiltrating lymphocytes (LA-TIL), induced by culture of peripheral blood lymphocytes and TIL respectively with rIL-2 showed high NK and LK activities and remarkable anti-autologous tumor ability.

Antineoplastic Agents↗
PubMed1991

Differentiation of central lung tumor from postobstructive lobar collapse by rapid sequence computed tomography.

When central lung tumor is associated with lobar collapse, discrimination of tumor from atelectasis is important for determining operability or for field planning should radiation therapy be utilized. Because tumor and collapsed lung have similar attenuation values, they cannot always be distinguished with usual contrast-enhanced CT. The article describes separation of tumor from collapsed lung by means of RSCT with bolus injection of nonionic contrast medium. With this technique collapsed lung is appreciably enhanced, but tumor enhancement is slow and minimal. The difference is most marked from 40 seconds to 2 minutes after scanning begins. This phenomenon is probably related to the marked increase in blood flow per unit area of collapsed lung due to crowding of relatively large pulmonary arteries compared to tumor blood supply through small bronchial arteries. Tumor can also be delineated from atelectasis by differences in internal structure with this technique.

Aged↗
PubMed1991

[The characteristics of nasopharyngeal carcinoma cell strain against chemotherapeutic agents and cytokines].

The closed relationship between nasopharyngeal carcinoma (NPC) and Epstein-Barr virus (EBV) has been pointed out. However, it still remains unclear whether the EBV becomes a true factor in initiation and promotion in NPC or not, because of difficulty to establish this cancer strain. We succeeded to establish the EBV-genome positive cell line (NPC-TY861) in 1986, which had been derived from the tissues of nasopharyngeal carcinoma. In this study, the characteristics of this cell strain were investigated by tests for sensitivity against several kinds of anti-cancerous agents and immuno-activated cells. The sensitivity tests revealed that NPC-TY861 cell line was extremely sensitive to PEP, ADR, VCR, LPA and CDDP with dose dependent manner. Furthermore, this cell strain had similar sensitivity to CDDP derivatives, such as 254-S, NK121, DWA2114R and CBDCA as KB cell strain did, which had been derived from an oral base cancer. The oral base cancer was a poorly differentiated squamous cell carcinoma histopathologically as same as NPC-TY861 cell strain. On the other hand, NPC-TY861 cell strain showed no sensitivity to cytokines, TNF and IFN, which have direct anti-cancerous actions. NPC-TY861 cell strain had resistant to NK cells and much more resistant to LAK cells derived from IL-2 than Daudi cell strain did. In order to elucidate the oncogeneous mechanism in NPC, it is indispensable to study the character of this cell strain and it would promise to get a new effective therapeutic method for the patient with NPC, who has bad prognosis.

Antineoplastic Agents↗
PubMed1991

[Comparative studies of diagnosis with US or CT of the cervical lymph node metastases in head and neck cancer].

Correct assessment of lymph node metastasis in the head-neck region is very important for management of head and neck cancer. 233 lymph nodes were removed by radical neck dissection from 12 cases with cancer in the head and neck region, who did not undergo any preoperative treatment, and the materials were histopathologically examined. The histopathological findings were compared with preoperative US and CT findings. Histopathologically, 26 lymph nodes were found positive for metastasis and the remaining 207 lymph nodes, negative. US detected 45 (19%) of 233 lymph nodes before operation, and 40 of the 45 lymph nodes (89% : 40/45) were qualitatively correctly diagnosed. CT detected 21 lymph nodes (19%), 16 of which were qualitatively correctly diagnosed (76% : 16/21). Of 26 lymph nodes which were histopathologically involved, 19 lymph nodes were correctly diagnosed by US and 11 by CT. Possible reasons explaining the superiority of US to CT in terms of diagnostic reliability are the following: 1) US demonstrates more clearly the existence of lymph nodes than CT. 2) US is more reliable for measuring sizes of lymph nodes than CT. In literature, CT has been often reported to be useful to diagnose cervical lymph node involvement in individual cases. However, we insist that it is necessary to diagnose individual lymph nodes strictly for correct assessment of the reliability of image diagnosis.

Female↗
PubMed1990

[Clinical studies of malignant tumors in the nasal cavity].

Nineteen cases with malignant tumors in the nasal cavities have been treated at the department of otolaryngology, Yokoharma City University, during the 10 years from 1978 to 1987. 1. Cases were 8 males and 11 females, and their ages ranged from 27 to 84 years (Mean age: 64.6). 2. In the histological classification, 9 cases were the epithelial malignant tumors (squamous cell carcinoma 5; adenoid cystic carcinoma 2; transitional cell carcinoma 1; malignant pleomorphic adenoma 1), 9 cases were non-epithelial malignant tumors (malignant melanoma 6; malignant lymphoma 2; olfactory neuroblastoma 1), and one case was unclassified malignant tumor. 3. Cases with epithelial malignant tumors showed better prognosis after treatment of surgical and radiation therapy. But those of non-epithelial malignant tumor were worse. 4. A very rare case with malignant pleomorphic adenoma, originated at the lateral nasal wall was reported and its clinical features and treatment were discussed. This tumor has not been reported up to the present in Japan.

Adult↗