Biomedical subjects

M V Kondashevskaya

Publications and source records attributed to M V Kondashevskaya.

5 recordsLinked to original sources

PubMed2006

Effect of single and repeated administration of high-molecular-weight heparin in low doses on brain content of neurotransmitters in Wistar rats.

We demonstrated high sensitivity of the brain neurotransmitter system to high-molecular-weight heparin in Wistar rats. Single and repeated administration of heparin modulated the content of monoamines and neurotransmitter amino acids in the hippocampus, striatum, and cerebral cortex. Antistress activity of high-molecular-weight heparin and its positive effects on memory under experimental conditions suggest that this preparation in low doses can be used in the therapy of neurological and age-related diseases.

Animals↗

PubMed2006

Hematological parameters in wistar rats in conditions of stress and treatment with heparin.

The effects on hematological measures of emotional tension arising during the formation of food-procuring behavior in a multiple-choice maze and in conditions of pain (injection of 0.85% physiological saline i.m., 0.3 ml, daily for five days) were studied in Wistar rats. Physiological saline and training of intact animals led to increases in RBC volume and mean corpuscular hemoglobin content, accompanied by significant increases in plasma corticosterone levels. At the same time, neither heparin (standard unfractionated heparin, Serva, doses of 64 and 640 IU/kg, daily for five days) nor training of heparinized rats produced any changes in these measures. The high levels of organization of the acquired behavior and the working capacity seen in heparinized rats were evidence for improvements in both the associative properties of conditioned reflex activity and the mechanisms supporting stable reproduction of the acquired habit. After administration of heparin, rats lost all types of manifestation reflecting psychoemotional tension as generally seen in animals in the active (administration of physiological saline) and passive (intact rats) controls.

Analysis of Variance↗

PubMed2005

Comparative characterization of cytokine production by concanavalin A-activated splenocytes from BALB/c and C57BL/6 mice after cold exposure.

The level of cytokines produced by ConA activated splenocytes was studied in male BALB/c and C57Bl/6 mice after single and repeated cold exposure (-20 degrees C, 3 min). Single cold exposure significantly decreased IL-2, -3, -4, -5, -10, -12, IFN-gamma production in BALB/c mice and decreased IL-2 content and increased TNF-alpha level in C57Bl/6 mice. Repeated cold exposure normalized the content of IL-2, -4, -10, -12, and IFN-gamma in BALB/c mice, which reflects the development of adaptive immune reactions. In C57Bl/6 mice IL-2, -3, -5, -10, -12, and IFN-gamma production remained significantly decreased, which attested to dysadaptive processes.

Animals↗

PubMed2004

Active control and its stress effects.

The effects of 5-fold injections of normal saline were studied in Wistar rats in two conditioned reflex situations: defense and alimentary. Intact animals developed conditioned pain avoidance reflex in repeated tail-flick test after 60 presentations over 3 days. Two injections of normal saline were sufficient for the formation of a stable negative stress status in experimental rats, which manifested by a drastic increase in tactile sensitivity persisting for 5 days after discontinuation of injections. Only 15% experimental rats vs. 40% intact animals developed the food-procuring response in a multialternative maze. Presumably, repeated injections of normal saline should be regarded as a potent negative stress factor modulating animals behavior, and these signs should be taken into consideration, when interpreting the effects observed in pharmacological investigations.

Animals↗

PubMed2000

New aspects of heparin effects.

The effects of a 5-day heparin treatment (10 kD, 64 IU/kg, intraperitoneally) on food-procuring behavior and spatial memory in a 12-arm radial maze were studies on Wistar rats. The maximum reinforcement scores in heparinized rats were attained by day 7 and in control rats only by day 16. In total, 75% heparinized and 45% control rats successfully learned the task for 24 days. On day 25 the contents of major transmitters and their metabolites in various brain structures and in the small intestine of control and experimental rats were determined. The rats treated with heparin showed increased concentrations of norepinephrine in the hypothalamus, homovanillic acid in the striatum, and serotonin in the small intestine. Our findings indicate that heparin exhibits a wide range of activities in addition to its anticoagulant effect.

Animals↗