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Biomedical subjects

M Wasik

Publications and source records attributed to M Wasik.

At least 91 records · Page 5Linked to original sources
PubMed1978

The effect of ATG treatment on LyMIF and LyMSF production in mixed lymphocyte cultures of rabbits after renal allotransplantation.

Rabbit lymphocytes, settled up in microcappillary tubes migrate into culture medium. In the previous communication we have shown that migration of mixture of lymphocytes derived from two blood donors was significantly inferior in 73% of cultures as compared to the corresponding monocultures. 4% of mixed cultures exhibited significant enhancement of migration and in 23% no effects were seen. Use of lymphocytes from rabbits 4-6 days after kidney allotransplantation and their corresponding donors resulted in conversion of this ratio. Percentage of cultures exhibiting enhancement of migration (production LyMSF) rose to 39, 46% of cultures were inhibited (produced LyMIF) and 15% did not show any significant effect. Treatment of kidney recipients with ATG exerted strong inhibitory effect on the appearance of LyMSF and on the appearance of LyMIF in mixed cultures. These results suggested that LyMIF and LyMSF were produced by separate cell populations.

Animals↗
PubMed1978

Study of antibody dependent cellular cytotoxicity (ADCC). II. ADCC activity in renal graft recipients and acute accelerated graft rejection.

ADCC activity in 27 cadaver renal allograft recipients was studied. All the patients were given standard immunosuppressive treatment. Significant positive correlation between high ADCC activity during first 5 days after grafting and accelerated rjection crisis was found. The high positive ADCC test may signalize the possibility of acute accelerated rejection of renal allograft.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1978

Study of antibody-dependent cellular cytotoxicity (ADCC). I. Lymphocyte-mediated cytotoxicity by lymphocytes from healthy individuals.

Lymphocytes from 32 healthy blood donors were determined for their ability to mediate the cytolysis of antibody-coated target cells. As target cells a murine lymphoma cells the cytolysis of antibody-coated target cells. As target cells a murine lymphoma cells L1210 were used. Heat-inactivated rabbit anti-L1210 antiserum was source of antibody. ADCC activity was determined at following effector to target cell ratsio: 100:1, 30:1, 10:1, 3:1, 1:1. Lymphocytes of males had significantly higher activity as compared to female ones. The individual fluctuation among both groups was not statistically significant.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1978

Study of antibody dependent cellular cytotoxicity (ADCC). III. Preliminary findings concerning the influence of immunosuppressive drugs.

The present studies were undertaken to characterize cytotoxic activity of lymphocyte-(ADCC) in the course of primary glomerulonephritis and to evaluate the influence of some of the immunosuppressive drugs on lymphocytes of examined patients. 18 patients were studied; 12 of them were treated by combined therapy with prednisone, azathioprine and chlorambucil. Results were analyzed on the basis of healthy individuals ADCC activity. In the 11 treated patients significant decrease of ADCC activity was observed while non-treated group exhibited as high activity as healthy individuals. Which of given drugs is responsible for the decreased ADCC activity, remains questionable and is currently under the study.

Adult↗
PubMed1978

Immunosuppressive effect of concanavalin-A. II. In vivo suppression of cytotoxic lymphocytes generation in alloimmunized mice.

An examination was carried out of the in vivo effect of Concanavalin-A (Con-A) on the induction of secondary cytotoxic response (CL) to allogeneic spleen cells in mice. CL responses were suppressed by one i.v. injection of 200 micrograms of Con-A one day before first or second immunization or two i.v. injections of Con-A, 200 micrograms each, given simultaneously with immunization. Single dose of 200 microgram of Con-A given simultaneously inhibited anamnestic CL response only partially, to the range observed in the primary response.

Animals↗
PubMed1976

Further studies on the influence of calf alpha-globulin on survival of skin allografts in mice.

A single injection of 10 mg of alpha-globulin fraction isolated from calf serum prolonged survival of skin allografts in mice provided the preparation was injected two days before grafting. An immunosuppressive effect was also obtained by injecting alpha-globulin in a total dose of 13 mg in the second week after grafting in mice pretreated with antithymocyte serum; On the other hand, such an effect was not obtained when alpha-globulin was injected in the early days after grafting in doses of up to 25 mg per mouse.

Alpha-Globulins↗
PubMedDate not supplied

Influence of promethazine on immune reactions. I. Characterization of promethazine effects on natural killer cell-mediated cytotoxicity.

Promethazine has been shown to possess definite immunosuppressive activity in clinical and experimental organ transplantation. However, there are few data concerning the mechanism of its influence on immune reactions. In the present studies promethazine was shown to inhibit natural killer cell-mediated cytotoxicity in vitro. Combined analysis of 51Cr-release and single-cell assays revealed that this agent affects some processes involved in delivering the 'lethal hit' but not the binding of target cells nor the recycling capacity of effector cells. The possible mechanism of promethazine action at the cellular level is discussed.

Cells, Cultured↗