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Biomedical subjects

M Weigert

Publications and source records attributed to M Weigert.

At least 37 records · Page 2Linked to original sources
PubMed1979

A search for hapten-binding mouse plasmacytoma proteins.

Six hundred and twelve mouse plasmacytomas were screened for hapten binding by using eleven different bacteriophage-hapten conjugates (phage T4 conjugated with haptens NP, NIP, DIP, DNP,BOC-ABA-Tyr, ABA-NP, ABA-MIP, ABS-HOP, PAB-HOP, penicillin G, cloxacillin). Fifteen ascites fluids (2.4%) inactivated at least one of the phage conjugates at a high dilution indicating binding. The specificity of these reactions was studied by titrating one ascites fluid with phage conjugates carring unrelated haptens, and by inhibiting the phage inactivation with free haptens. Of the 15 myeloma proteins, 10 had high titers (at least 30 times higher than the ascites fluid background) with the NIP-cap phage or the NP-cap phage or both. Four had high titers with the DNP-cap phage and one with the ABA-MIP phage. Thirteen of the 15 myeloma proteins were IgA, one was IgM and one IgG2b.

Animals↗
PubMed1979

Total replacement of the femur and its adjacent joints.

We report three cases of alloplastic replacement of the femur and its adjacent joints. The indications and operative technique are described. The femoral shaft of polyethylene can be manufactured within a short time and may be individually adapted to standard replacements of the hip and knee. The operation may be performed even on older patients, it preserves the limb and allows early mobilisation with full weight bearing.

Aged↗
PubMed1979

Myeloma proteins from NZB and BALB/c mice: structural and functional differences.

Structural and functional analyses of myeloma immunoglobulins from inbred BALB/c mice and humans have provided important insights into the structure of the antibody molecule and the expression and evolution of antibody genes. One important question concerning these analyses is whether the myeloma process selects, in a nonrandom manner, the lymphocytes to be transformed. The availability of myeloma tumors in a second inbred strain of mouse, NZB, permits us to approach this question. In this respect. the NH2-terminal amino acid sequences of 27 kappa light chains as well as data relating to the antigen-binding properties and immunoglobulin class distribution of NZB myeloma proteins are presented and compared with similar data from the BALB/c mouse. These studies suggest that the myeloma proteins from the BALB/c and NZB mice constitute two populations of immunoglobulins with distinct functional and structural properties. The implication of this observation are discussed.

Amino Acid Sequence↗
PubMed1979

Comparisons of myeloma proteins from NZB and BALB/c mice: structural and functional differences of heavy chains.

The N-terminal sequences from heavy variable regions of 47 myeloma proteins of the NZB mouse have been analyzed. Sixteen of these VH regions have unblocked alpha amino groups and have been analyzed over their N-terminal 20 residues by automatic sequence analysis. These sequence data along with the antigen-binding profiles and immunoglobulin class distribution are compared with comparable data from BALB/c myeloma proteins. These comparisons suggest that the NZB and BALB/c populations of myeloma proteins are distinct from one another. The genetic implications of this conclusion are discussed.

Amino Acid Sequence↗
PubMed1979

[Fractures of the forearm in children; operative treatment (author's transl)].

The knowledge of the special characteristics of fractures in childhood determine the method of treatment. Within 2 1/2 years 162 fractures of the forearm were treated, 23 of them by open reposition. The specialities of the different anatomical regions and the best way of treatment are discussed. Conservative and operative methods complement each other to obtain the best result while the risk for the little patient is reduced.

Adolescent↗
PubMed1979

[Different indications for endoprotheses of the hip joint (ceramics (Al2O3) or internal shells?) (author's transl)].

The internal shell endoprothesis for the hip joint after Wagner has considerably limited the indications for the implantation of ceramic endoprotheses to patients under 60 years, because less bone substance has to be sacrificed. Furthermore there are indications for ceramic endoprotheses in cases of substance defects of the femoral head and neck, to some extent also in cases of dysplasia and protrusio acetabuli, and by loosening traditional total hip endoprotheses.

Adult↗
PubMed1979

[Inter- and subtrochanterie fractures treated by Ender's method (author's transl)].

308 patients with intertrochanteric and subtrochanteric femoral fractures were treated by Ender's method of intramedullary fixation from June 1975 to September 1978. The average age was 78,4 years, the mortality rate was 16,3 per cent. There was no infection of the bone and no non-union. In 78 per cent of re-examined patients the hip function was good and very good. The treatment of old patients with this operation is the method of choice.

Adult↗
PubMed1979

[Rare injuries of the Achilles tendon (author's transl)].

The problems of Achilles tendon rupture become more important, because the number of sport injuries is increasing. The typical rupture (transverse rupture) is well known. However in literature the medial rupture between muscle and tendon of the musc. gastrocnemius and the longitudinal rupture of the Achilles tendon are rarely mentionaled. This article describes the special symptoms of these injuries and their therapy.

Achilles Tendon↗
PubMed1978

Chromosomal locations of mouse immunoglobulin genes.

The chromosomal locations of the structural genes coding for the constant portions of mouse heavy (H) and light chain immunoglobulins were studied by molecular hybridization techniques. Complementary DNA probes containing the constant-region sequences of kappa and lambdaI light chain and alpha, gamma2b, and mu heavy chain mRNAs were annealed to a large excess of DNA from a series of eight mouse-human hybrid cell lines that are deficient for various mouse chromosomes. The lines were scored as positive when a high proportion of a probe annealed and negative when an insignificant proportion annealed. Some lines were clearly negative for H and lambda and clearly positive for kappa. Others were positive or intermediate for lambda, positive for kappa and negative for H. Still others, including a line that was selected for the absence of the mouse X chromosome, were positive for all immunoglobulin species. These results demonstrate that the Clambda, Ckappa, and CH genes are located on different autosomes in the mouse. In contrast, the three heavy-chain families exhibited consistently uniform hybridization results, suggesting that the genes for Calpha, Cgamma, and Cmu are located on the same chromosome. A comparison of karyotypic data with hybridization data has limited the possible locations of the Ig genes to only a few chromosomes.

Animals↗
PubMed1978

Plasmacytomas of the NZB mouse.

Plasmacytomas were readily induced in NZB mice by three i.p. inoculations with pristane (2, 6, 10, 14-tetramethylpentadecane). In comparison with comparable induction regimens in BALB/c mice a) the latent period for plasmacytoma development was significantly longer in NZB's; b) the frequency of IgA paraproteins produced by NZB tumors was much reduced and that of IgG (IgG1, IgG2a, IgG2b, IgG3Y was significantly increased; and c) the frequency of carbohydrate-binding paraproteins was 10-fold lower. There was a high frequency of primary ascites containing more than one paraprotein, but none of 25 such tumors produced more than one immunoglobulin class on serial transplantation.

Animals↗