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M Yutsudo

Publications and source records attributed to M Yutsudo.

At least 55 records · Page 3Linked to original sources
PubMed1991

Cloning and characterization of human papillomavirus type 52 from cervical carcinoma in Indonesia.

Human papillomavirus (HPV) type 52 from a cervical carcinoma in Indonesia was molecularly cloned and characterized. By hybridization with cervical carcinoma DNAs from Indonesian patients, HPV 52 was detected in 3 of 52 cases (6%), whereas HPV 16 and 18 were detected in 8 and 7 cases, respectively (15% and 13%). Sequence analysis revealed that the E6-E7 ORFs contained several DNA binding motifs (Cys-X-X-Cys) like previously sequenced HPVs. The E6 ORF also contained splice donor and acceptor signals, which may allow the expression of E6* protein. The E7 ORF encoded an amino acid sequence that is conserved in some DNA tumor viruses and is involved in binding to Rb protein and in cellular transformation. Transfection of a subgenomic fragment of HPV 52 under the control of a heterologous promoter showed that the E7 ORF alone induced anchorage-independent growth of established rodent cells and immortalized primary rat embryo fibroblasts (REF), and that in cooperation with activated ras, it induced malignant transformation of REF. The E6 ORF also induced, less efficiently, anchorage-independent growth. These results strongly suggest that HPV 52, like HPV 16 and 18, has oncogenic potential.

Amino Acid Sequence↗
PubMed1991

Progression of rat embryo fibroblast cells immortalized with transforming genes of human papillomavirus type 16.

To clarify the mechanism of cell transformation by human papillomavirus type 16 (HPV16), we constructed recombinant murine retroviruses containing various subgenomic fragments of the HPV16 early region and examined their abilities to transform rat fibroblasts in primary culture. The E7 ORF, but not the E6 ORF, immortalized cells in primary culture, but the recombinant retrovirus containing both the E6 and E7 ORFs did not transform them. However, after long-term cultivation of cells immortalized by E6 and E7 ORFs, some cells became transformed. During this progression, the amounts of viral mRNA and E7 protein did not change and virus rescued from progressed cells could not transform cells in primary culture, suggesting that some changes in cellular genes, but not viral genes, cause malignant progression of immortalized cells. During this process, the expression of c-K-ras mRNA and its product increased but that of c-myc mRNA did not.

Animals↗
PubMed1991

Isolation of cellular revertants from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16.

Three revertants defective in the ability to form colonies in semisolid medium were isolated from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16 (HPV16). These revertants appeared to be defective in a cellular factor(s) necessary for transformation by HPV16-E6E7 genes since they still expressed a comparable amount of HPV16-E6E7 mRNA and E7 protein to the parental cells, harbored rescuable transforming virus, and were resistant to retransformation by HPV16-E6E7 genes. All these reverted phenotypes of the three mutants were recessive on somatic cell hybridization with normal cells, because all the hybrids showed transformed phenotypes.

Animals↗
PubMed1991

Very high incidence of germ cell tumorigenesis (seminomagenesis) in human papillomavirus type 16 transgenic mice.

Human papillomavirus type 16 (HPV16) is frequently found in carcinomas and precancerous lesions of the uterine cervix and is thought to be closely associated with carcinogenesis in these regions. However, the transforming activity of the E6 and E7 genes in vivo has not been characterized. To investigate this function, we produced transgenic mice carrying HPV16 E6 and E7 open reading frames. We obtained five transgenic founders and established three transgenic lineages. We observed testicular tumors of germ cell origin in mice of all three lineages. Morphological studies showed that these tumors were a type of seminoma. Both testes of all tumor-bearing mice were affected with this type of tumor. Strikingly, in one lineage, all of the male mice developed this tumor. On Northern (RNA) analysis, a high level of expression of HPV mRNA was detected in these tumors. These results suggest that transforming genes of HPV16 have transforming activity in vivo and preferential effects on germ cells in the testis.

Animals↗
PubMed1990

Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV).

Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. Molecular biological analysis of three revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. These findings suggest the existence of a specific mechanism(s) for elimination of the v-abl oncogene by segregation, mutation, or gene rearrangement in these cells.

Abelson murine leukemia virus↗
PubMed1989

A rat mutant cell clone showing temperature-dependent transformed phenotypes with functional expression of the src gene product.

The cellular mutant B814 isolated from a Fischer rat cell line shows temperature-sensitivity of focus formation on infection with Moloney murine sarcoma virus (Mo-MSV) and Rous sarcoma virus (RSV). An RSV-transformed clone (S814-2) isolated from B814 cells shows temperature-sensitive transformed phenotypes for morphology, growth in soft agar, and glucose uptake. The expression, phosphorylation, and tyrosine kinase activity of pp60v-src in S814-2 were not affected at the nonpermissive temperature, and virus rescued from this clone had wild-type transforming ability, suggesting that a cellular factor altered in S814-2 is responsible for the cellular steps of transformation after the function of pp60v-src. In addition, the cellular 36K protein, a possible candidate as a target of pp60v-src, was phosphorylated at the nonpermissive temperature in S814-2, indicating that phosphorylation of the 36K protein is not correlated with transformed phenotypes.

Animals↗
PubMed1989

Types of human papillomavirus isolated from Japanese patients with epidermodysplasia verruciformis.

Virological studies were performed on 12 patients with epidermodysplasia verruciformis (EV). Three types of lesions were observed: red plaques, pityriasis versicolor (PV)-like macules and plane warts. Human papillomavirus (HPV) 14, 20 and 21 were isolated from the plaques, HPV 3, 14 and 38 from flat warts and HPV 5, 12, 17, 20 and 38 from PV-like lesions. No clear relationship could be established between the different lesions and the types of HPV. Types 17 and 20 have been isolated most frequently from Japanese EV patients and HPV 5, frequently detected in other countries, is less common, whereas HPV 8 has not been isolated. Skin cancers occurred in six of the cases (50%) and all had benign lesions that were PV-like. At least one type of HPV 5, 17 or 20 could be isolated from these benign lesions and HPV 17 or 20 detected in the cancers. These three types of HPV in EV patients appear to be involved in the malignant transformation.

Adult↗
PubMed1989

A unique verrucous anogenital tumor associated with type 6b-related human papillomavirus.

Multiple dark brown papillomatous tumors, showing some histological features of verrucous carcinoma or giant condyloma, developed mainly in the anogenital region of a Japanese woman. The tumors first appeared when she was 51 years old and annoyed her for over 20 years with several recurrences without any frank malignant transformation, after surgery. Immunohistochemically, papillomavirus genus-specific antigen was demonstrated only in small foci of the lesions resected at first operation. Southern blot analysis revealed human papillomavirus type 6b-related DNA in a surgically resected specimen. The possible role of the human papillomavirus in the genesis of this unique tumor is discussed.

Aged↗
PubMed1989

[Oncogene of human papillomavirus].

Although certain types of human papillomavirus (HPV) have been supposed to be associated with carcinogenesis of cervix, a definitive conclusion has not yet been reported. To show that HPV is a causative agent of cervical cancer, we performed several lines of experiments and obtained the results described below. 1) In attempting to identify an activated oncogene(s), we showed that cellular DNAs from 8 cervical carcinomas induced transformation of NIH 3 T 3 cells and, in all cases, HPV sequences were retained in the secondary transformants. 2) Using a retrovirus vector system, we showed that HPV-16 had transforming activity for mouse or rat established cells. The transforming genes are located in the E6 and E7 ORF: the former governs tumorigenicity in nude mice and the latter influences cell growth properties such as saturation density, colony formation in soft ager and immortalization. These results strongly suggest that specific types of HPV are involved in carcinogenesis of the cervix. However, HPV only may not be sufficient to induce carcinomas, because even the E6 and E7 ORFs of HPV-16 could not transform rat primary cells immediately after infection, but could do so after a long latency. Evaluation of the change during the latency is very important to clarify the mechanism of cervical carcinogenesis.

Animals↗
PubMed1988

Functional dissociation of transforming genes of human papillomavirus type 16.

Human papillomavirus (HPV) type 16 is thought to be responsible for development of cervical carcinomas, but the mechanism of its carcinogenic action is unknown. To determine which viral genes are involved in cellular transformation, we constructed recombinant murine retrovirus DNAs containing various subgenomic fragments of the HPV 16 early region and examined their transforming activities. The results show that the E6 and E7 ORFs are the transforming genes of HPV 16; the former governs the tumorigenicity in nude mice and the latter influences cell growth properties such as saturation density and colony formation in soft agar. There may also be a tumor-suppressing gene in the E1-E2 ORF region, because the tumorigenic activity of recombinant DNA containing the E1 ORF and the 5' portion of the E2 ORF in addition to the E6 and E7 ORFs was much lower than that of recombinant DNA containing only the E6 and E7 ORFs.

Animals↗
PubMed1988

Temperature-sensitive cellular mutant for expression of mRNA from murine retrovirus.

The cellular mutant B812 isolated from a Fisher rat cell line shows temperature sensitivity of focus formation induced by various retroviruses such as recombinant murine retrovirus containing the middle T gene of polyomavirus (PyMLV), Kirsten murine sarcoma virus, Moloney murine sarcoma virus, and recombinant murine retrovirus containing the src gene of Rous sarcoma virus. B812 cells, however, show normal ability to proliferate and synthesize protein at the nonpermissive temperature, suggesting that their mutation is in a gene specifically concerned with the process of transformation by retroviruses. In this work, experiments with hybrids of mutant and wild-type cells showed that the temperature-dependent defect of this mutant was complemented by wild-type cells. To determine the step of transformation that is restricted at the nonpermissive temperature in B812, we examined the expressions of the oncogene (middle T antigen) in no. 7 (wild-type cells) and B812 cultures infected with PyMLV (the chimeric retrovirus containing the middle T gene of polyomavirus) at the permissive and nonpermissive temperatures. Middle T-associated protein kinase activity, the expression of middle T antigen, and PyMLV-specific mRNA were reduced at the nonpermissive temperature in B812 cultures infected with PyMLV. However, integration of PyMLV into the chromosomal DNA of the mutant was not affected at the nonpermissive temperature. These results suggest that B812 cells have a mutation affecting the expression of viral mRNAs from integrated proviral DNA at the nonpermissive temperature.

Animals↗
PubMed1987

Purification and properties of a non-histone chromatin protein with a molecular weight of 38,000 daltons specific for transformed rat cells.

Non-histone chromatin proteins prepared from a normal rat cell line (No. 7) and the cells transformed with Rous sarcoma virus (RSV) (s7-1) were compared by means of reverse-phase high performance liquid chromatography (reverse-phase HPLC), followed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed that several proteins were specifically present in the transformed cell chromatin. A specific non-histone chromatin protein with a molecular weight of 38,000 daltons, 38K protein, was purified as a single species from s7-1 cells. This 38K protein was only detected in the transformed state of the cells transformed with a temperature-sensitive (ts) mutant of the src gene and the mutant cells which showed temperature sensitivity as to the transformation with wild type RSV.

Animals↗
PubMed1987

Human papillomavirus type 17 transcripts expressed in skin carcinoma tissue of a patient with epidermodysplasia verruciformis.

Certain types of human papillomavirus (HPV) are involved in skin carcinogenesis in epidermodysplasia verruciformis. However, no gene or gene product of HPV associated with skin carcinogenesis has yet been identified. Here, we report HPV-17 transcripts expressed in skin carcinoma tissue of an epidermodysplasia verruciformis patient infected with HPV-17. Further, we show that one of these transcripts was localized to a portion of the genome which contains the 3' open reading frames of the early region (E2, E3, E4 and E5). The analogous region in bovine papillomavirus type I has been shown to contain a transforming gene (Nakabayashi et al., 1983; Sarver et al., 1984; Yang et al., 1985a).

Bovine papillomavirus 1↗
PubMed1987

New human papillomavirus sequences in female genital tumors from Japanese patients.

Tissues from 52 cervical carcinomas, 15 cervical intraepithelial neoplasias III (CINs III), and 3 vulvar carcinomas were examined for the presence of human papillomavirus (HPV) sequences by Southern blot hybridization with HPV 16 or 18 DNA as the probe. HPV 16 or 18 DNA was detected in only 17 cervical carcinomas (33%), 5 CINs III (33%), and 1 vulvar carcinoma (33%). These frequencies are lower than those reported by others. However, new, as yet unidentified, HPVs were also detected at rather high frequency under less stringent conditions of hybridization using HPV 16 and 18 DNAs as probes. These HPVs did not hybridize with HPV 6, 11, 16, or 18 under stringent conditions, and were different from two other known types of genital tumor-associated HPVs, HPV 31 and HPV 33, judging from the patterns of their restriction enzyme digests. The total frequencies of HPV sequences were 48% (25/52) for cervical carcinomas, 53% (8/15) for CINs III, and 67% (2/3) for vulvar carcinomas.

Carcinoma↗
PubMed1986

Human papillomavirus type 38 isolated from patients with epidermodysplasia verruciformis.

A new type of human papillomavirus (HPV), HPV-38b, was isolated from patients with epidermodysplasia verruciformis and was molecularly cloned. This HPV was shown by hybridization experiments to have almost no sequence homology with other types of reported HPVs, but did show homology with HPV-38, which was recently isolated from a melanoma. A physical map and a rough genetic map of the organization of this HPV are presented.

Adult↗
PubMed1986

Epidemiologic aspects of epidermodysplasia verruciformis (L-L 1922) in Japan.

A survey of epidermodysplasia verruciformis (EV), a skin disease caused by human papillomavirus (HPV), was made by means of a questionnaire sent to 92 university hospitals. Replies were obtained from 68 hospitals (74% recovery) reporting 66 patients. Fewer patients were reported from north-eastern Japan than from south-western Japan. Many EV patients were from families of consanguineous marriages (44%), showing a high incidence of intra-familial onset after consanguineous marriages. The complication of malignant tumors was observed in 36 of 62 cases (58%). Malignant tumors of the skin developed at an early age in EV patients. These cancers developed predominantly in exposed areas of the skin (72%). The time from onset of skin lesions to the onset of cancer seemed to be related to the nature of the lesions in EV patients. These findings suggest that the interaction of HPVs, ultraviolet rays and host factors is associated with the development of skin carcinomas.

Adolescent↗
PubMed1985

Human papillomavirus type 17 DNA in skin carcinoma tissue of a patient with epidermodysplasia verruciformis.

Epidermodysplasia verruciformis (EV) is a serious skin disease caused by certain types of human papillomavirus (HPV), because the flat wart-like lesions of EV very frequently change to malignant squamous cell carcinoma. The relation between HPV and skin carcinoma was examined by studies on an EV patient who had a squamous cell carcinoma. HPV-17 was isolated from EV lesions of this patient. With HPV-17 DNA as a probe, cellular DNA prepared from the carcinoma tissue was analyzed by Southern blot hybridization. Results showed that cells contained about 100 copies of monomeric and oligomeric extrachromosomal HPV DNA. These results suggest that HPV-17 is involved in skin carcinogenesis in EV.

DNA, Viral↗