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Biomedical subjects

M Yutsudo

Publications and source records attributed to M Yutsudo.

67 records · Page 4Linked to original sources
PubMed1983

Rat mutant cells showing temperature sensitivity for transformation by wild-type Moloney murine sarcoma virus.

To clarify the cellular target(s) of onc gene products of Moloney murine sarcoma virus (Mo-MSV), we isolated seven mutant cells that exhibit temperature sensitivity for transformation by wild-type Mo-MSV from Fischer rat cell line. Five strains of these mutant cells showed normal virus production at the nonpermissive temperature when infected with Mo-MSV, suggesting that viral replication is not affected by these cellular mutations. Four of these mutants were also temperature sensitive (ts) for transformation by Kirsten murine sarcoma virus (Ki-MSV), whereas the other three mutants were not ts, suggesting that our mutants isolated with Mo-MSV can be divided into two classes as regards temperature sensitivity to transformation by Ki-MSV.

Animals↗
PubMed1983

Isolation and characterization of host range mutants of avian sarcoma virus.

Two host range mutants of avian sarcoma virus (ASV) were isolated from a clone of the Bratislava 77 strain, subgroup C (B77-C). An HR- mutant was obtained by adaptation to duck cells and showed almost the same efficiencies of transformation (e.o.t.) and growth in cultures of chick embryo fibroblasts (CEF) and duck embryo fibroblasts (DEF). An HR+ mutant showed lower e.o.t. and slower growth on DEF than on CEF. Characterization of these mutants and wild-type B77-C showed that (i) unlike the host range mutants reported previously, these mutants and wild-type B77-C have common antigenicity, (ii) the number of infective centres is almost the same for the two mutants and the wild-type, and so the adsorption and integration of these viruses are similar, and (iii) the content of viral RNA in DEF infected with HR- is increased and decreased in the case of HR+ compared with the wild-type.

Animals↗
PubMed1983

[Cellular factors participating in cell transformation].

To clarify the cellular target(s) of onc gene products of Moloney murine sarcoma virus (Mo-MSV), we isolated eight mutant cells that exhibit temperature-sensitivity for transformation by wild type Mo-MSV from F2408 (Fischer rat cell line). These mutant cells showed normal growth and normal Mo-MSV production at the nonpermissive temperature, suggesting that cellular and viral replication are not affected by these cellular mutations. These mutant cells are divided into 3 classes on the bases of temperature-sensitivity for transformation by other tumor viruses such as Kirsten sarcoma virus, Rous sarcoma virus (SRD strain), Fujinami sarcoma virus and Abelson leukemia virus. These results have suggested that several kinds of cellular factors are concerned in transformation by Mo-MSV.

Animals↗
PubMed1982

New human papilloma virus isolated from epidermodysplasia verruciformis lesions.

Human papilloma virus (HPV) was isolated from red plaques of a patient (N. F.) with epidermodysplasia verruciformis. Electron microscopic examination showed characteristic particles of papilloma virus as icosahedrons about 45 nm in diameter. DNA was extracted from these particles, and closed-circular DNA (Form I) was purified by centrifugation in CsCl containing ethidium bromide. The molecular weight of the DNA was about 5.0 x 10(6). A physical map of the HPV DNA was constructed using several restriction enzymes. The restriction endonuclease cleavage pattern of the HPV DNA was different from those of other types of HPV reported thus far, suggesting that the isolate was a new, as yet unclassified, HPV.

Adult↗
PubMed1979

Regulation of imm gene expression in bacteriophage T4-infected cells.

Two polypeptides (imm-a and imm-b) which are not induced by an immunity mutant T4Dimm2 but by a wild-type strain T4D were identified by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. Their mol. wt. were 77 000 and 45 000, respectively. These polypeptides exhibited a similar kinetic pattern of synthesis. Within a few minutes p.i. the primary phage established the system that inhibited imm gene expression of superinfecting phage. This was shown by measuring both the phenotypic expression of immunity and the synthesis of imm gene polypeptides. The expression of two other immediate-early genes, namely genes s and 30, and early gene 33, was not affected by primary infection.

Genes, Viral↗
PubMed1979

Heterogeneous RNA of the Prague strain of Rous sarcoma virus caused by undiluted passages.

The size of genomic RNA in PR-RSV A passaged in chick embryo fibroblasts (CEF) or quail embryo fibroblasts (QEF) was determined by gel electrophoresis. The results showed that 3 undiluted passages resulted in heterogeneity of RNA. The heterogeneity of the smaller incomplete RNAs in the virus stock was decreased by diluted passage or cloning, but RNA of the b subunit size and a subunit RNA of complete genome size were relatively stable. These heterogeneous RNAs were characterized by hybridization analysis. The RNAs from 4 peaks hybridized with both cDNAtotal and cDNAsrc to appreciable extents, indicating that they were derived from viral RNA and that at least some of them contained the src sequence. This finding and the failure to isolate a td mutant from the undiluted-passaged virus stock or from some subclones that had a and b subunits of RNA indicate that the td virus was only a minor constituent of the incomplete virus population caused by undiluted passages. Some viruses with incomplete RNA in the virus stock could produce foci with the aid of td B77 or RAV-60. The emergence of rd viruses by undiluted passages was indicated.

Animals↗
PubMed1979

Establishment of cell lines from the wild rodent Millardia meltada and tests for endogenous virus.

Four cell lines were established from the wild rodent, Millardia meltada; an untransformed cell line (MM-D), a non-producer (NP) cell line transformed with murine sarcoma virus (MM-CL4), and SV40-transformed cell lines (MM-8 MM-663). MM-D is epithelioid and contact-inhibited, whereas MM-CL4, MM-8, and MM-663 are fibroblastic and grow piling up. No endogenous virus was induced in Millardia cell lines by a long-term culture with various inducers. Millardia cell DNA has no nucleotide sequence homology with cDNA of either murine leukemia virus or rat endogenous virus.

Animals↗
PubMed1977

[Immunohistologic study of the chicken kidney].

After absorption with adult Chicken liver homogenate, antisera produced in Rabbits against the DOC-soluble fractions of the sediments obtained after centrifugation of adult Chicken kidney homogenates, react with at least two types of antigens. One, found to be kidney-specific, is localised at the apical part of the cells of the proximal segment of the secretory tubules. The other, also present in the digestive tract, is detectable in the ureter and its derivatives, the collecting tubules by which it seems to be excreted. Despite the lack of organ specificity the latter represents an interesting marker for studying the early steps of the development of the renal collecting system.

Animals↗