Liquid Granada medium for detection of group B streptococci.
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Culture Media↗
Biomedical subjects
Manuel de la Rosa-Fraile
Publications and source records attributed to Manuel de la Rosa-Fraile.
Toscana virus in Spain.
Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.
Animals↗
[Preliminary assessment of new antigen detection methods for prompt diagnosis of syncytial respiratory virus].
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Antigens, Viral↗
[Use of MPO chromogenic culture medium for routine processing of urine cultures].
BACKGROUND: The chromogenic culture medium, MPO, was compared to culture on CLED (cystein, lactose, electrolyte-deficient) agar for the detection, enumeration and identification of urinary tract pathogens. METHODS: A total of 1,080 clinical urine specimens were assessed. All samples were inoculated in MPO and CLED using the calibrated loop method. RESULTS: Among 145 positive urine samples, 171 strains of bacteria were isolated (111 Escherichia coli, 26 Enterococcus spp., 12 Proteus spp., 10 Enterobacteriaceae from the Klebsiella-Enterobacter-Serratia group, 5 Pseudomonas aeruginosa, 4 Streptococcus agalactiae, 3 Staphylococcus spp. and 4 Candida albicans. For all samples, enumeration of microorganisms was comparable with the two media studied. Identification was also similar, except for 6 cases in which Enterococcus spp. were only detected with the chromogenic medium. CONCLUSIONS: Overall urine culture results with MPO chromogenic medium were similar to those obtained with CLED, making it a feasible alternative to the standard medium. Moreover, use of a chromogenic technique implies a significant reduction in workload, since additional tests to identify the microorganisms isolated are not needed in most cases.
Bacteria↗