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Toscana virus in Spain.

Sara Sanbonmatsu-Gámez·Mercedes Pérez-Ruiz·Ximena Collao·María Paz Sánchez-Seco·Francisco Morillas-Márquez·Manuel de la Rosa-Fraile·José Maria Navarro-Mari·Antonio Tenorio
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Abstract

Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.

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AnimalsAntibodies, ViralFemaleHumansImmunoglobulin GInsect VectorsMalePhlebotomusPhlebotomus FeverPhylogenyPrevalenceSandfly fever Naples virusSequence Analysis, DNASpainViral ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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Sara Sanbonmatsu-Gámez, Mercedes Pérez-Ruiz, Ximena Collao, María Paz Sánchez-Seco, Francisco Morillas-Márquez, Manuel de la Rosa-Fraile, José Maria Navarro-Mari, Antonio Tenorio. 2005. Toscana virus in Spain.. https://doi.org/10.3201/eid1111.050851

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