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Biomedical subjects

Marco Ferrari

Publications and source records attributed to Marco Ferrari.

At least 19 recordsLinked to original sources
PubMed2007

Effect of oxygen inhibition on composite repair strength over time.

The study was aimed at examining whether an oxygen inhibition layer is required for bonding a repairing to a pre-existing composite, and to determine the time required for free radicals within a composite substrate to decay to the extent that the composite repair strength drops significantly. Ten slabs of Gradia Direct Anterior (GC Corp.) were divided into (1) control group: an interfacial oxygen inhibition layer was created by applying and light-curing two layers of bonding resin (D/E Resin, Bisco) to the slabs surface in atmospheric air; (2) experimental group: the absence of an interfacial oxygen inhibition layer was obtained by light-curing the second bonding resin layer in a nitrogen atmosphere. After 1 and 2 h, 1, 14, and 30 days of air storage, a composite repair was layered over the bonding resin. Microtensile bond strengths were measured and statistically analyzed. The curing atmosphere was not a significant factor for bond strength (p = 0.82), and time and curing atmosphere-time interaction were significant (p < 0.001). The 30 day-strengths were the lowest (p < 0.05). An oxygen-inhibited layer is not initially required for bonding to resin composite, and it takes more than 14 days before the bond strength between a pre-existing and a fresh composite drops.

Biomechanical Phenomena↗
PubMed2006

Neuropsychological findings in young-adult stroke patients.

There are few data on neuropsychological deficits in young-adult stroke patients. This study investigates cognitive conditions in a young-adult stroke population, as well as tasks that detect their neuropsychological impairment. Forty 18- to 47-year-old stroke patients, and a matched control group, completed a neuropsychological battery to evaluate deficits related to cognition, daily activities and mood. Patients performed worse than controls; five patients were classified as demented, three had global cognitive impairment and eight partial cognitive impairment. Cognitive impairment was more closely associated with reduced performance of daily activities than with motor deficits.

Activities of Daily Living↗
PubMed2006

Three-dimensional finite element analysis of strain and stress distributions in endodontically treated maxillary central incisors restored with different post, core and crown materials.

OBJECTIVES: The present comparative analysis aimed at evaluating which combination of restorative materials resulted in the most homogeneous stress and strain distributions. METHODS: A three-dimensional finite element analysis was performed. All the nodes on the external surface of the root were constrained in all directions. Eighteen experimental models with different material properties and configurations were simulated. An arbitrary load of 10N was applied at 60 degrees angle with tooth longitudinal axis on the palatal surface of the crown. Von Mises (equivalent stresses) energetic criterion was chosen. RESULTS: In all the models the values of both strain and stress recorded at the middle third of the buccal aspect of the root surface were at their maxima. On the contrary, the minimum values were noticed at level of both the apical portion of the post and the root apex. The maximum stresses were evidenced at level of the cemento-enamel junction (CEJ) on both the buccal and palatal aspects of root cement and dentin. Stress progressively decreased from the outer to the inner part of the root and from the CEJ towards the incisal margin of the crown as well. SIGNIFICANCE: The results of the present study would allow clinicians to make an informed choice from among available materials to restore endodontically treated teeth.

Cementation↗
PubMed2006

Human CD4+CD25+ regulatory T cells selectively express tyrosine hydroxylase and contain endogenous catecholamines subserving an autocrine/paracrine inhibitory functional loop.

CD4+CD25+ regulatory T lymphocytes (Tregs) are specialized T cells playing a key role in the control of immune homeostasis. Here, we show that human Tregs constitutively express tyrosine hydroxylase (TH, EC 1.14.16.2), the rate-limiting enzyme in the synthesis of catecholamines, and contain substantial amounts of dopamine, norepinephrine, and epinephrine, which are released upon treatment with reserpine. Catecholamine release results in reduced production of interleukin-10 and transforming growth factor-beta by Tregs, and in down-regulation of Treg-dependent inhibition of effector T-lymphocyte (Teff) proliferation, which occurs without affecting the production of tumor necrosis factor-alpha or interferon-gamma. Tregs and Teffs express on the cell membrane both D1-like and D2-like dopaminergic receptors to a similar extent (12%-29% of the cells). Catecholamine-dependent down-regulation of Tregs is, however, selectively reversed by pharmacological blockade of dopaminergic D1-like receptors, which in Tregs only (and not in Teffs) are also expressed at the level of mRNA and are functionally coupled to intracellular production of cAMP. These findings indicate that in human Tregs endogenous catecholamines subserve an autocrine/paracrine loop involving dopaminergic pathways and resulting in down-regulation of Treg function.

Autocrine Communication↗
PubMed2006

Immediate and 24-hour evaluation of the interfacial strengths of fiber posts.

This study examined the contribution of testing time on the interfacial strengths of fiber posts to radicular dentin. Twenty-five fiber posts were luted to root segments with resin cements and a zinc phosphate cement. Immediately upon setting of the materials, the roots were sectioned into slices and randomly divided into two subgroups, depending on testing time (immediate vs. 24-hours), for push-out test. The results showed an improvement on the interfacial strength after 24 hours of storage. When data were pooled for each cement, the interfacial strengths (MPa) were: Optibond Solo Plus/Nexus: 10.3+/-3.8a; zinc phosphate cement: 10.1+/-2.7a; Multilink: 9.6+/-3.8ab; RelyX Unicem: 7.1+/-4.9bc; AllBond 2/Duolink: 6.7+/-3.5c (groups with same superscript letter were not statistically significant). It is concluded that bond strength can increase during the first 24 hours and that the interfacial strength is predominantly contributed by frictional retention.

Analysis of Variance↗
PubMed2006

The effect of immediate versus delayed cementation on the retention of different types of fiber post in canals obturated using a eugenol sealer.

Post can be placed immediately after the completion of the endodontic procedure or at a later stage after setting of the sealer. In this in vitro study, post space preparation and post cementation were performed immediately (group 1), 24 h (group 2), and 7 days (group 3) after root filling. A control group with no root canal obturation was included (group 4). Each group was further divided into three subgroups, according to the post type and to the materials used for luting the post. A push-out test was performed to evaluate post-root canal dentin interfacial strength. Differences among experimental groups were analyzed with two-way ANOVA and the Tukey test. Significant differences (p < 0.05) in interfacial strengths were observed for the factors post cementation interval and post type. Clinicians should be careful in performing immediate cementation of fiber posts, as delayed cementation shows higher post-dentin interfacial strengths irrespective of the post type.

Analysis of Variance↗
PubMed2006

Fracture resistance and failure patterns of endodontically treated mandibular molars restored using resin composite with or without translucent glass fiber posts.

The elastic modulus of the restorative material is important in restoring endodontically treated teeth. This study aimed to compare the fracture resistance and failure patterns of 90 mandibular molars restored using resin composites with or without fiber posts, with respect to the number of residual cavity walls. Five restoration types were performed corresponding to different wall defects (groups 1-5). Groups were divided in two subgroups corresponding to the use or absence of fiber posts. Teeth were loaded and resistance of specimens was measured as the axial compressive load to cause fracture and macroscopic fracture patterns were observed. One way ANOVA revealed a significant difference in fracture resistance (p < 0.001). Tukey post hoc test also revealed significant differences between groups as samples restored with fiber posts exhibited mostly restorable fractures. It was concluded that the resistance of endodontically treated mandibular molars restored with composite resins is mainly affected by the number of residual walls. Using fiber-reinforced posts optimized fracture patterns.

Acrylic Resins↗
PubMed2006

Ultrasound effects after post space preparation: An SEM study.

The aim of this study was to evaluate the effect of ultrasonic treatment on occlusion of dentine tubules in root canal walls after post space preparation in endodontically treated teeth. Twenty-four premolars were instrumented and filled using warm vertical condensation; after post space preparation, they were divided into two groups. The control group was treated using the etching procedure. The experiment samples were treated with EDTA irrigation and ultrasound activation for 30 s before the etching procedure. The roots were divided and the canal walls were examined under SEM at 1000x magnification. The debris and open tubule marks were observed at 2, 6, and 10 mm levels using a three-step scale and the differences in marks among the groups were tested for statistical significance. The following were observed: (a) A decrease in debris and open tubule marks in the samples treated with ultrasounds and the control group (p < 0.05), (b) no significant differences between the three levels of post space in debris and open tubule marks in the experiment samples, and (c) significant differences between the apical and coronal levels in debris and open tubule marks in the control group.

Dental Etching↗
PubMed2006

Effect of hygroscopic expansion on the push-out resistance of glass ionomer-based cements used for the luting of glass fiber posts.

This study examined the contribution of hygroscopic expansion of glass-ionomer (GIC) and resin modified glass-ionomer (RMGIC) luting cements to the push-out resistance of fiber posts. Glass fiber posts were luted to post spaces using different cements. Experimental specimens were stored in water, while control specimens were desiccated and stored in mineral oil to eliminate water from intraradicular dentinal tubules and/or the external environment that could have contributed to hygroscopic expansion of the cements. Thin slice push-out tests revealed no difference in retention strengths of resin composite cements that were stored in water or oil. Conversely, GIC and RMGIC cements exhibited increased retention strengths after water sorption. As unfavorable cavity geometry is taxing to dentin bond integrity in root canals, a strategy that relies on increasing the frictional resistance to post dislodgement via delayed hygroscopic expansion of glass-ionomer based materials may be a more pragmatic approach to fiber post retention.

Acrylic Resins↗
PubMed2006

Foxp3 expressing CD4+ CD25+ and CD8+CD28- T regulatory cells in the peripheral blood of patients with lung cancer and pleural mesothelioma.

The role of T regulatory (Treg) cells in human cancer has not yet been clarified. We assessed the presence and function of CD4+ and CD8+ Treg cell subsets in the peripheral blood of patients with lung cancer (LC) and pleural mesothelioma (PM). We found a low but significant increase in the number of CD4+ T cells with phenotype and functional features of Treg cells in LC patients compared to normal healthy controls (NHC). Furthermore, total CD4+ T cells from LC patients proliferated less than cells from controls, suggesting that the increase in the CD4+ Treg cell pool has functional importance. LC patients also showed an expansion of the CD8+CD28- T cell subset and these cells expressed Foxp3 mRNA, as recently observed in alloantigen-specific CD8+CD28- T suppressor cells. No variation of peripheral Treg cell subsets was found in patients with PM, a disease with a predominantly localized nature. However, the lack of correlation between cancer stage and the number or the function of peripheral Treg cells in LC patients refuted the hypothesis that these cells are involved in tumor spreading. A possible involvement of the peripheral Treg cell pool in cancer development and/or in inducing systemic immunosuppression in LC patients can be hypothesized.

Aged↗
PubMed2006

Angiotensin II type 1 receptor expression on human leukocyte subsets: a flow cytometric and RT-PCR study.

The renin-angiotensin system plays a key role in the regulation of cardiovascular functions and in particular angiotensin II type 1 receptor (AT1R)-operated pathways are involved in the modulation of inflammation in the vascular wall. In the present study we assessed the pattern of expression of AT1Rs on different human circulating leukocyte subsets. Venous blood was obtained from healthy male subjects. Leukocyte subsets were purified by immunomagnetic cell sorting or identified in whole blood using multiparametric cytometric analysis. RT-PCR analysis showed that AT1R mRNA was expressed in polymorphonuclear leukocytes (PMNs), monocytes, B-lymphocytes, and, to a lesser extent, T-lymphocytes. Flow cytometric analysis revealed that the frequency of expression of AT1Rs was: PMNs>monocytes>or=B-lymphocytes>>T-lymphocytes, while receptor density per positive cells was: PMNs>or=B-lymphocytes>T-lymphocytes>or=monocytes. AT1Rs are expressed on PMNs, monocytes, T- and B-lymphocytes, however the expression pattern is peculiar to each subset, possibly suggesting distinct roles in the various cell types. Investigating the expression and the functional role of AT1Rs on circulating leukocyte subsets, as well as their possible modifications in disease conditions before and after pharmacological treatments, is likely to provide novel clues to the comprehension of the mechanisms involved in the therapeutic efficacy of currently available agents.

Adult↗
PubMed2006

The effect of application sustained seating pressure on adhesive luting procedure.

OBJECTIVES: To evaluate the effect of short versus long application seating pressure on the bond strength of resin blocks, luted with a dual-cured resin cement (Panavia F) to pre-coated or non pre-coated dentin with an hydrophobic light-cured adhesive (Clearfil Protect Bond). METHODS: Sixteen non-carious human third molars were randomly divided into six Groups (four teeth each). Cylindrical composite blocks were luted with Panavia F (Group Ia) and with Clearfil Protect Bond with Panavia F (Group IIa) and seating pressure was applied for 5s. In Groups Ib and IIb, the two bonding procedures were respectively repeated, but the resin cylinder was maintained under constant pressure during the entire 3min polymerization period for the resin cement. After storing in distilled water for 24h, 0.9mmx0.9mm sticks were produced from these luted specimens for microtensile bond testing and SEM examination. RESULTS: The use of Clearfil Protect Bond with Panavia F produced higher bond strengths than the use of Panavia F (p<0.05). Extending the time of pressure application up to 3min increased the bond strength (p<0.001) and improved the integrity of the interfacial quality. SIGNIFICANCE: The application of sustained seating pressure during luting procedures and the additional use of a hydrophobic light-cured adhesive both improve the final bond strength of the resin cement.

Cementation↗
PubMed2006

Is an oxygen inhibited layer required for bonding of resin-coated gutta-percha to a methacrylate-based root canal sealer?

Removal of the oxygen inhibition layer from the surface of resin-coated gutta-percha cones during packaging has been hypothesized for their weak adhesion to a methacrylate-based root canal sealer, resulting in their frequent delamination from the sealer after root canal obturation. This study examined the feasibility of creating oxygen inhibition layers on resin-coated gutta-percha cones via the adjunctive application of a dual-cured dentin adhesive just before bonding. Composite cylinders were bonded with EndoREZ to flat, resin-coated gutta-percha disks and similar disks that were post-treated with Prime&Bond NT Dual Cure adhesive and stressed to failure using a modified microshear testing design. Although shear strengths for both groups were relatively low, a 5-fold increase in shear strength was observed after adhesive application, with complex interfacial failures instead of complete sealer delamination from the resin-coating. In-situ dentin adhesive application appears to have merits in enhancing the coupling of resin-coated gutta-percha to methacrylate sealers.

Analysis of Variance↗
PubMed2006

Improving the quality of the quartz fiber postcore bond using sodium ethoxide etching and combined silane/adhesive coupling.

The influence of different silane/dentin adhesives on the microtensile bond strengths of a hybrid composite to sodium ethoxide-etched quartz fiber posts was investigated. A prehydrolyzed silane was applied without an adhesive and compared to two-component systems in which hydrolysis of the silane occurred after mixing with the acidic monomer present in the dentin adhesives. Nanoleakage along post/core interfaces was examined after silver nitrate tracer penetration using scanning electron microscopy. Higher interfacial strengths and more uniform adaptation between the etched fiber post surface and the composite core were recorded after post treatment with silane/adhesive couplings. The combination of silane with two-step self-etch adhesives improves the chemical retention of composites around etched fiber posts with minimal nanoleakage. Although the one-step self-etching adhesive/silane combination enhances post adhesion, the extensive nanoleakage provides channels for rapid water sorption that may expedite hydrolytic degradation of the post/core interface.

Composite Resins↗
PubMed2006

Bond strength performance of different resin composites used as core materials around fiber posts.

OBJECTIVES: To evaluate the microtensile bond strengths of different resin composites used as core materials around fiber posts. METHODS: Forty DT Light-Posts (RTD) were randomly divided into eight groups, according to the resin composite used. They included two core materials specifically developed for core build-up--Group 1: Core-Flo (Bisco Inc.) and Group 2: UniFil Core (GC Corp.); three hybrid composites--Group 3: Tetric Ceram (Ivoclar-Vivadent), Group 4: Gradia Direct (GC Corp.), Group 5: Bisfil 2B (Bisco, Inc.); and three flowable composites--Group 6: AEliteflo (Bisco, Inc.), Group 7: Filtek Flow (3M ESPE) and Group 8: UniFil Flow (GC Corp). A cylindrical plastic matrix was placed around the silanized post and filled with the respective resin composite. Each bonded post provided five to eight sticks for microtensile testing. Each stick was loaded to failure under tension at a cross-head speed of 0.5mm/min. One-way ANOVA and Tukey's test were used for statistical analysis. Scanning electron microscopy (SEM) was used to evaluate the interface of the fractured sticks. RESULTS: Resin composites exhibited a significant influence on microtensile bond strength (p<0.05). Core-Flo showed the highest bond strength (11.00+/-0.69 MPa) although it was not statistically significantly different from all groups, except from the flowable composites. Under SEM, all the composites adapted well to the fiber post, with a variable extent of voids observed along the fractured composite interfaces. SIGNIFICANCE: Although good adaptation to the post surface was achieved, bond strength to fiber post remains relatively weak. Core build-up and hybrid composites are better alternatives to flowable composites as core build-up materials.

Barium Compounds↗
PubMed2006

Evaluation of the biomechanical behavior of maxillary central incisors restored by means of endocrowns compared to a natural tooth: a 3D static linear finite elements analysis.

OBJECTIVE: The present study aimed at evaluating different restoring configurations of a crownless maxillary central incisor, in order to compare the biomechanical behavior of the restored tooth with that of a sound tooth. MATERIALS AND METHODS: A 3D FE model of a maxillary central incisor is presented. An arbitrary static force of 10 N was applied with an angulation of 125 degrees to the tooth longitudinal axis at level of the palatal surface of the crown. Different material configurations were tested: composite, syntered alumina, feldspathic ceramic endocrowns and glass post resorations with syntered alumina and feldspathic ceramic crown. RESULTS: High modulus materials used for the restoration strongly alter the natural biomechanical behavior of the tooth. Critical areas of high stress concentration are the restoration-cement-dentin interface both in the root canal and on the buccal and lingual aspects of the tooth-restoration interface. Materials with mechanical properties underposable to that of dentin or enamel improve the biomechanical behavior of the restored tooth reducing the areas of high stress concentration. SIGNIFICANCE: The use of endocrown restorations present the advantage of reducing the interfaces of the restorative system. The choice of the restorative materials should be carefully evaluated. Materials with mechanical properties similar to those of sound teeth improve the reliability of the restoartive system.

Aluminum Oxide↗
PubMed2006

Effects of adhesive systems and luting agents on bonding of fiber posts to root canal dentin.

The study evaluated the influence of different luting materials on the microtensile bond strength of glass fiber posts to root canal dentin. Thirty extracted maxillary premolars were endodontically treated, and the roots were prepared for post cementation using the FRC Postec system (Vivadent). Two luting materials (Multilink, Vivadent and Clearfil Photo Core, Kuraray) were used in combination with three adhesive: Multilink Primer (Vivadent), Clearfil Photo Bond, and Clearfil New Bond (Kuraray). A composite build-up was performed around the root to provide adequate gripping during testing. Specimens were cut to obtain beams with the post in the center and with the radicular dentin overlaid by the composite build-up on each side. Microtensile testing was performed with a universal testing machine at a cross-head speed of 0.5 mm/min. The failure mode was classified under a stereomicroscope and four representative beams of each group were selected for SEM analysis. Bond strength data that were analyzed with two-way ANOVA and Student-Newman-Keuls multiple comparisons tests revealed that adhesive systems, luting materials, and the interaction between these two factors significantly influenced the bond strength results (p < 0.01). Multilink applied with its own adhesive system obtained the best results, while the lowest bond strength was achieved with clearfil photo core in combination with multilink primer.

Adhesives↗