Biomedical subjects

Mark C Pitter

Publications and source records attributed to Mark C Pitter.

4 recordsLinked to original sources

PubMed2006

Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields.

We report bright-field and dark-field surface-plasmon imaging using a modified solid immersion lens and a commercial objective of moderate NA in the epi configuration. The contrast and resolution are extremely good, giving well-resolved images of protein monolayers both in air and in water. We also describe a two-part solid immersion lens that allows the sample to be moved without degrading the image quality in any observable way. The merits of the two-part lens are discussed and compared to commercially available microscope objectives. Finally, we introduce a simple Green's function model that illustrates the key features of both bright-field and dark-field surface-plasmon imaging.

Equipment Design↗

PubMed2004

Full-field heterodyne interference microscope with spatially incoherent illumination.

A heterodyne interference microscope arrangement for full-field imaging is described. The reference and object beams are formed with highly correlated, time-varying laser speckle patterns. The speckle illumination confers a confocal transfer function to the system, and by temporal averaging, the coherence noise that often degrades coherent full-field microscope images is suppressed. The microscope described is similar to a Linnik-type microscope and allows the use of high-numerical-aperture objective lenses, but the temporal coherence of the illumination permits the use of a low-power achromatic doublet in the reference arm. The use of a doublet simplifies alignment of the microscope and can reduce the cost. Preliminary results are presented that demonstrate full-field surface height precision of 1 nm rms.

Journal Article↗

PubMed2003

Remote photoacoustic detection of liquid contamination of a surface.

A method for the remote detection and identification of liquid chemicals at ranges of tens of meters is presented. The technique uses pulsed indirect photoacoustic spectroscopy in the 10-microm wavelength region. Enhanced sensitivity is brought about by three main system developments: (1) increased laser-pulse energy (150 microJ/pulse), leading to increased strength of the generated photoacoustic signal; (2) increased microphone sensitivity and improved directionality by the use of a 60-cm-diameter parabolic dish; and (3) signal processing that allows improved discrimination of the signal from noise levels through prior knowledge of the pulse shape and pulse-repetition frequency. The practical aspects of applying the technique in a field environment are briefly examined, and possible applications of this technique are discussed.

Journal Article↗

PubMed2003

Characterization of layered scattering media using polarized light measurements and neural networks.

Measurements of the spatial distributions of polarized light backscattered from a two-layer scattering medium are used to train a neural network. We investigated whether the absorption coefficients and thickness of the layer can be determined when the scattering properties are known. When determining the absorption of the upper layer or the layer's thickness, polarized light measurements provide better performance than unpolarized measurements, demonstrating the sensitivity of polarized light to superficial tissue. Determination of the lower layer's absorption coefficient is not improved by polarized light measurements. Prior knowledge of the tissue under investigation is also beneficial because errors are reduced if the range of absorption or thickness is restricted.

Algorithms↗