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Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields.

Jing Zhang·Mark C Pitter·Shugang Liu·Chungwah See·Mike G Somekh
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Abstract

We report bright-field and dark-field surface-plasmon imaging using a modified solid immersion lens and a commercial objective of moderate NA in the epi configuration. The contrast and resolution are extremely good, giving well-resolved images of protein monolayers both in air and in water. We also describe a two-part solid immersion lens that allows the sample to be moved without degrading the image quality in any observable way. The merits of the two-part lens are discussed and compared to commercially available microscope objectives. Finally, we introduce a simple Green's function model that illustrates the key features of both bright-field and dark-field surface-plasmon imaging.

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Equipment DesignEquipment Failure AnalysisImage EnhancementLensesMicroscopyPhantoms, ImagingReproducibility of ResultsSensitivity and SpecificitySurface Plasmon ResonanceJournal Article

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Jing Zhang, Mark C Pitter, Shugang Liu, Chungwah See, Mike G Somekh. 2006-11-01. Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields.. https://doi.org/10.1364/ao.45.007977

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