Mervyn W Billinghurst | PubMed Health

PubMed2004

Alternative quality control for technetium-99m IDA complexes.

A quality control procedure for 99mTc-IDA complexes based on the use of C18 Sep-pak cartridges is developed and the validation of the procedure presented. C18 Sep-pak cartridges are pretreated by washing with 95% ethanol followed by 10(-3) N hydrochloric acid. A small amount of the 99mTc-IDA complex is applied, washed with 10(-3) N hydrochloric acid and eluted with 95% ethanol. The radiochemical purity values obtained for 99mTc-mebrofenin and 99mTc-disofenin using this Sep-pak procedure are comparable to those obtained using the standard two strip (ITLC-SG/100% methanol, ITLC-SA/20% saline) procedure.

Chromatography, Thin Layer↗

PubMedDate not supplied

A study of factors affecting the labelling of tartrate with 188Re and the transchelation of the 188Re from the tartrate to a protein.

The formation of 188Re-tartrate for use in transchelation reactions and the transchelation of the 188Re onto albumin was studied. Two labelled tartrate products were separated using a non-traditional mobile phase on ITLC strips. Tartrate labelling yield increases with pH but so does the instability when the product is exposed to air. Lower pH's are preferred when oxygen-free labelling conditions can be achieved. Higher tin levels protect against air oxidation. Stability of the Re-tartrate complex is supported by addition of ascorbic acid and ferrous sulphate, however both these agents decreased the rate of the formation of the Re-tartrate complex. The labelling efficiency of a perrhenate solution decreased with the time for which it is stored prior to the labelling reaction, depending on the radioactive concentration.Re-albumin transchelation efficiency increases with the tartrate concentration, while increased stability of the Re-tartrate complex lowers the transchelation yields of Re-albumin.

Chelating Agents↗