PubMed Health⌕ Search

Biomedical subjects

Min Qian

Publications and source records attributed to Min Qian.

At least 19 recordsLinked to original sources
PubMed2026

Effect of protective ventilation throughout the intubation period on perioperative oxygenation in patients undergoing MIDCABG: a randomised controlled trial.

INTRODUCTION: Minimally invasive direct coronary artery bypass grafting (MIDCABG) requires prolonged one-lung ventilation (OLV), increasing postoperative pulmonary complications (PPCs) risk. We investigated whether protective lung ventilation (PLV) throughout intubation benefits MIDCABG patients. METHODS: In this single-center randomized study, MIDCABG patients received PLV (low tidal volume of 6-8&#x2009;mL&#xb7;kg-1, PEEP of 6&#x2009;cm H2O, alveolar recruitment maneuvers) or conventional mechanical ventilation (CMV, tidal volume of 8-10&#x2009;mL&#xb7;kg-1, without PEEP or maneuvers) from tracheal intubation to extubation. The primary outcome was perioperative oxygenation, assessed by the PaO2/FiO2 ratio. RESULTS: Sixty patients (n = 30 per group) were enrolled. Compared with CMV, PLV improved PaO2/FiO2 ratios (mean difference at OLV60: 34.56&#x2009;mmHg; 95% CI: 11.78-57.33; p&#x2009;<&#x2009;0.01), shortened median durations of postoperative mechanical ventilation (median difference: -4.5&#x2009;h, 95% CI: -8.5 to -0.5; p&#x2009;=&#x2009;0.013) and hospital stay (median difference: -3.0&#x2009;days, 95% CI: -5.0 to -1.0; p&#x2009;=&#x2009;0.019). PLV also reduced driving pressure, airway pressure and intrapulmonary shunt during OLV (all p&#x2009;<&#x2009;0.05). Desaturation occurred in 23.3% of CMV patients and 13.3% of PLV patients (p&#x2009;=&#x2009;0.506). Hemodynamic parameters were generally comparable between groups, except for lower MPAP and PVRI in the PLV group during OLV and after ICU admission (p&#x2009;<&#x2009;0.05). The incidence of PPCs did not differ between groups. CONCLUSIONS: In patients undergoing MIDCABG, PLV applied throughout intubation improved perioperative oxygenation and shortened the duration of postoperative mechanical ventilation and hospital stay, but did not reduce PPCs. CLINICAL TRIAL REGISTRATION: ChiCTR1900022005.

Humans↗
PubMed2006

Microsomal prostaglandin E synthase-1 deficiency is associated with elevated peroxisome proliferator-activated receptor gamma: regulation by prostaglandin E2 via the phosphatidylinositol 3-kinase and Akt pathway.

mPGES-1 (microsomal PGE synthase-1) is an inducible enzyme that acts downstream of cyclooxygenase (COX) and specifically catalyzes the conversion of prostaglandin (PG) H(2) to PGE(2) under basal as well as inflammatory conditions. In this study, using mouse embryo fibroblasts (MEFs) isolated from mice genetically deficient for the mPges-1 gene, we show basal elevation of peroxisome proliferator-activated receptor gamma (PPARgamma) expression (protein and mRNA) and transcriptional activity associated with reduced basal PGE(2). We further show that basal mPGES-1-derived PGE(2) suppresses the expression of PPARgamma through a cAMP-independent pathway involving phosphatidylinositol 3-kinase and Akt signaling. Using specific PPARgamma agonist (rosiglitazone), PPARgamma ligand (15-deoxy-Delta12,14-PGJ(2)), and PPARgamma inhibitor (GW9662), we confirm that activation of PPARgamma blocks interleukin-1beta-induced up-regulation of COX-2, mPGES-1, and their derived PGE(2). Furthermore, we demonstrate that up-regulation of PPARgamma upon genetic deletion of mPGES-1 is responsible for reduced COX-2 expression under basal as well as interleukin-1beta-stimulated conditions. This study provides evidence for the first time that mPGES-1 deletion not only decreases proinflammatory PGE(2) but also up-regulates anti-inflammatory PPARgamma, which has the ability to suppress COX-2 and mPGES-1 expression and PGE(2) production. Thus, mPGES-1 inhibition may limit inflammation by multiple mechanisms and is a potential therapeutic target.

Animals↗
PubMed2006

Osteoblastic cell response on fluoridated hydroxyapatite coatings.

Fluoridated hydroxyapatite (FHA) coatings were deposited onto Ti6Al4V substrates by sol-gel dip-coating method. X-ray photoelectron spectroscopy results showed that fluoride ions were successfully incorporated into the hydroxyapatite (HA) lattice structure. The dissolution behavior in Tris-buffered physiological saline indicated that all fluoridated HA coatings had lower solubility than that of the pure HA coating. The lowest solubility was obtained at fluoride ion concentrations of 0.8-1.1M. In vitro cell responses were evaluated with human osteosarcoma MG63 cells in terms of cell morphology, proliferation and differentiation (alkaline phosphatase activity and osteocalcin level). For all coatings tested, similar cell morphologies and good cell viability were observed. Coatings fluoridated to 0.8-1.1 had a stronger stimulating effect on cell proliferation and differentiation activities. The influences on cell phenotypes were attributed mainly to a combined ion effect of Ca, P and F released from the coating during dissolution. For the best dissolution resistance and cell activities, it is recommended that the molar level of fluoride ion be from 0.8 to 1.1, such that the coating takes the form of Ca(10)(PO(4))(6)(OH)(1.2-0.9)F(0.8-1.1).

Alkaline Phosphatase↗
PubMed2006

Shunting of prostanoid biosynthesis in microsomal prostaglandin E synthase-1 null embryo fibroblasts: regulatory effects on inducible nitric oxide synthase expression and nitrite synthesis.

Microsomal prostaglandin (PG) E synthase (mPGES)-1 is an inducible enzyme that acts downstream of cyclooxygenase (COX) and specifically catalyzes the conversion of prostaglandin (PG)H2 to PGE2, most prominently in inflammatory conditions. Specific inhibitors of mPGES-1 are not yet available, however, mice with genetic deletion of mPGES-1 have been generated that have given insight into the specific role of mPGES-1 in eicosanoid biosynthesis in vivo and in peritoneal macrophages. We created mouse embryo fibroblast (MEF) cell lines that would facilitate investigation of the effect of mPGES-1 genetic deletion on prostanoid biosynthesis in fibroblast lineage cells and its subsequent effect on the expression of inducible NOS (iNOS) and nitrite biosynthesis using cells derived from mPGES-1 wild-type (WT), heterozygous (Het), and null mice. The results show that genetic deletion of mPGES-1 results in a dramatic decrease in PGE2 production in Het and null MEFs under basal conditions and after stimulation with interleukin (IL)-1beta, suggesting that mPGES-1 is critically important for PGE2 production. Furthermore, we show that mPGES-1 gene deletion results in diversion of prostanoid production from PGE2 to 6-keto PGF1alpha (the stable metabolic product of PGI2; prostacyclin) in a gene dose-dependent manner in Het and null MEFs compared with their WT counterparts, suggesting a shunting phenomenon within the arachidonic acid (AA) metabolic pathway. In addition, we show that mPGES-1 gene deletion and subsequent decrease in PGE2 levels results in a differential induction profile of iNOS and nitrite levels (the stable breakdown product of nitric oxide (NO) in mPGES-1 WT MEFs compared with null MEFs. These results provide important information regarding the therapeutic potential for pharmacologic inhibition of mPGES-1 in inflammatory conditions.

Animals↗
PubMed2006

Uptake and translocation of Cd in different rice cultivars and the relation with Cd accumulation in rice grain.

The variations among six rice cultivars in cadmium (Cd) uptake and translocation were investigated with pot soil experiments. The results showed that only a very small portion (0.73%) of Cd absorbed by rice plant was transferred into grain. With regard to plant total Cd uptake, Cd concentrations and quantity accumulations in roots, stems and leaves, the differences among the cultivars (between the largest one and the smallest one) were less than one time. But for Cd concentrations and Cd quantity accumulations in the grains, the differences were more than five and eight times, respectively. With respect to Cd distribution portions in plant organs, the diversities among the cultivars were also small in roots, stems and leaves, but much larger in grains. Grain Cd concentrations correlated positively and significantly (P<0.01) with Cd quantity accumulations in plant, Cd distribution ratios to aboveground parts, and especially with Cd distribution ratios from aboveground parts to the grain. The results indicated that Cd concentration in rice grain was governed somewhat by plant Cd uptake and the transport of Cd from root to shoot, and in a greater extent, by the transport of Cd from shoot to grain. Cd was not distributed evenly in different products after rice grain processing. The average Cd concentration in cortex (embryo) was five times more than that in chaff and polished rice. With regard to Cd quantity accumulation in the products, near 40% in cortex (embryo), 45% in polished rice and 15% in chaff averagely.

Cadmium↗
PubMed2006

In vitro panning of a targeting peptide to hepatocarcinoma from a phage display peptide library.

Phage display technology has been used as a powerful tool in the discovery of ligands specific to receptor(s) on the surface of a cancer cell and could also impact clinical issues including functional diagnosis and cell-specific drug delivery. After three rounds of in vitro panning and two rounds of reverse absorption, a group of phages capable of addressing BEL-7402 enormously were obtained for further analysis. Through a cell-based ELISA, immunofluorescence, FACS, and in vivo binding study, WP05 (sequence TACHQHVRMVRP) was demonstrated to be the most effective peptide in targeting four kinds of liver cancer cell lines (BEL-7402, BEL-7404, SMMC-7721, and HepG2), but not the normal liver cell line HL-7702. In conclusion, the peptide WP05 which was screened by in vitro phage display technology was proved to be a targeting peptide to several common hepatocellular carcinoma cell lines.

Amino Acid Sequence↗
PubMed2006

Extraocular muscle morphogenesis and gene expression are regulated by Pitx2 gene dose.

PURPOSE: PITX2 gene dose plays a central role in Axenfeld-Rieger syndrome. The purpose of this study was to test the hypothesis that the effects of Pitx2 gene dose on eye development can be molecularly dissected in available Pitx2 mutant mice. METHODS: A panel of mice with Pitx2 gene dose ranging from wild-type (+/+) to none (-/-) was generated. Eye morphogenesis was assessed in animals with each Pitx2 gene dose. We also compared global gene expression in eye primordia taken from e12.5 Pitx2+/+, Pitx2+/-, Pitx2-/- embryos using gene microarrays. The validity of microarray results was confirmed by qRT-PCR. RESULTS: Morphogenesis of all extraocular muscle bundles correlated highly with Pitx2 gene dose, but there were some differences in sensitivity among muscle groups. Superior and inferior oblique muscles were most sensitive and disappeared before the four rectus muscles. Expression of muscle-specific genes was globally sensitive to Pitx2 gene dose, including the muscle-specific transcription factor genes Myf5, Myog, Myod1, Smyd1, Msc, and Csrp3. CONCLUSIONS: Pitx2 gene dose regulates both morphogenesis and gene expression in developing extraocular muscles. The expression of key muscle-specific transcription factor genes is regulated by Pitx2 gene dose, suggesting that sufficient levels of PITX2 protein are essential for early initiation of the myogenic regulatory cascade in extraocular muscles. These results document the first ocular tissue affected by Pitx2 gene dose in a model organism, where the underlying mechanisms can be analyzed, and provide a paradigm for future experiments designed to elucidate additional effects of Pitx2 gene dose during eye development.

Animals↗
PubMed2006

[Study on the molecular background of Del phenotype in Chinese population].

OBJECTIVE: To elucidate the molecular background of Del phenotype in the Chinese population and explore new Del alleles. METHODS: Five hundred and fifteen RhD negative blood samples was tested by Rh typing test, indirect antiglobulin test and adsorption and elution assay to screen the Del phenotype. DNA of all the Del samples was analysed by multiplex polymerase chain reaction (MPX PCR) for the presence of RHD and by sequence-specific primer polymerase chain reaction (PCR-SSP) for Del alleles: RHD 1227A and RHD 885T. Samples which showed the negative result by PCR-SSP, were additionally analysed by genomic DNA sequencing and cDNA sequencing. RESULTS: Seventy-nine Del samples were found by adsorption and elution assay. All these samples had RHD exons 3, 4, 5, 6, 7 and 9. Except 4 Del samples, other 75 Del samples carried the RHD 1227A allele. None of the samples had the RHD 885T allele. Four novel RHD alleles were found in these four Del sample. There were RHD 3G-->A (GenBank DQ310735), RHD 28C-->T, RHD 53T-->C (GenBank DQ451877,DQ451878), RHD 251T-->C (GenBank DQ310734). CONCLUSION: fnRh blood group system is very complex. New D variation phenotypes and new RHD alleles may be discovered ceaselessly.

Alleles↗
PubMed2005

[Changes in apoptosis-related genes expression profile in human breast carcinoma cell line Bcap-37 induced by flavonoids from seed residues of Hippophae Rhamnoides L].

BACKGROUND & OBJECTIVE: Hippophae rhamnoides L. possesses functions of antioxidation and radioprotection. This study was designed to investigate changes in apoptosis-related genes expression profile in human breast carcinoma cell line Bcap-37 induced by flavonoids from seed residues of Hippophae rhamnoides L. (FHR) with cDNA microarray, and to explore possible mechanism of signal transduction on apoptosis. METHODS: Total RNA was extracted from Bcap-37 cells before and after treatment of FHR. Two cDNA probes, labeled by Cy3-dUTP or Cy5-dUTP fluorescent dyes, were synthesized via reverse transcription, and hybridized with a microarray contained 13 824 human 14K cDNA. Differential gene expression profiles of FHR group and control group were analyzed by Genespring software. RESULTS: After treatment of FHR, 305 genes were up-regulated, and 361 were down-regulated; 32 apoptosis-related genes were differentially expressed, and accounted for 0.23% of the total genes in cDNA microarray. Of the 32 apoptosis-related genes, 25 were up-regulated (average Ratio: 3.071), and 7 were down-regulated (average Ratio: 0.418). Bioinformatic analyses showed that the 32 genes, including CTNNB1, TSSC3, IGFBP4, IGFBP6, GADD34, TNFRSF10B, Caspase-9, and PCNA, related with apoptosis of Bcap-37 cells when treated with FHR. CONCLUSION: Apoptosis of Bcap-37 cells induced by FHR relates with various genes through co-regulating of intracellular and extracellular signal transduction pathways.

Apoptosis↗
PubMed2005

[Selection of the peptides specifically binding to hepatoma by using phage display in vivo].

OBJECTIVE: To screen the peptides binding to hepatoma specifically. METHODS: Nude mice were inoculated with human tumor cells BEL-7402, then the Ph.D.-12 Phage Display Peptide Library was injected intravenously (tail vein) into mice. After 20 min the mice were sacrificed and the phage rescued from tumor tissues. All the tissues should be made an appraisal using immunohistochemistry and titering. The phage recovered from the tissues were amplified and purified then re-injected for next round screening. After screening for 3 rounds in vivo, the phage-peptides that homed to the tumor tissues or cells were obtained. Then these phage clones were sequenced to analyze the motif. All the sequenced clones were appraised by using cell ELISA and titering the distribution in vivo. RESULTS: Through the appraisal from vivo and vitro and peptides' sequences, several target motifs were preliminarily determined. CONCLUSIONS: Some phage-peptides which could specifically bind tumor cells or tissues can be selected successfully from the random twelve-peptide library by means of phage display in vivo.

Animals↗
PubMed2004

[The effect of disinfection on dimension stability of impressions].

PURPOSE: To measure the effect of disinfection on dimension stability of alginate, hydrocolloid, and Express putty impressions which were separately immersed in 2% glutaraldehyde solution and 1% 84 disinfector. METHODS: Alginate, hydrocolloid, and Express putty impressions were made by 20 mm x 20 mm x 40 mm standards test wares. There were 45 in each kind of test wares. They were divided into 9 groups and 5 pieces in every group. Among them, one group using clear water to flush acted as the matched control, the other groups received respectively 2% glutaraldehyde solution and 1% 84 disinfector immersed in 10 minutes, 20 minutes, 30 minutes. After two hours, the spiral instrument was used to measure the model. The experiment data were analyzed by using the SPSS 11.0 statistic software package. RESULTS: There was no significant difference in pure water flush and spraying treatment respectively. There was significant difference for alginate and hydrocolloid impression was immersed before and after. There was no significant difference for Express putty silicon rubber impression materials before and after immersion. There was no significant difference in 2% glutaraldehyde solution and 1% 84 disinfector when impressions were immersed in. CONCLUSION: Alginate, hydrocolloid and Express putty impressions immersed in 2% glutaraldehyde solution and 1% 84 disinfector separately for 20 minutes does not affect the accuracy, and can be used clinically.

Dental Disinfectants↗
PubMed2003

Delay of ZGA initiation occurred in 2-cell blocked mouse embryos.

One-cell mouse embryos from KM strain and B6C3F1 strain were cultured in M16 medium, in which 2-cell block generally occurs. Embryos of KM strain exhibited 2-cell block, whereas B6C3F1 embryos, which are regarded as a nonblocking strain, proceeded to the 4-cell stage in our culture condition. It is often assumed that the block of early development is due to the failure of zygotic gene activation (ZGA) in cultured embryos. In this study we examined protein synthesis patterns by two-dimensional gel electrophoresis of [35S] methionine radiolabeled 2-cell embryos. Embryos from the blocking strain and the nonblocking strain were compared in their development both in vitro and in vivo. The detection of TRC expression, a marker of ZGA, at 42 h post hCG in KM embryos developed in vitro suggested that ZGA was also initiated even in the 2-cell arrested embryos. Nevertheless, a significant delay of ZGA was observed in KM strain as compared with normally developed B6C3F1 embryos. At the very beginning of major ZGA as early as 36 h post hCG, TRC has already been expressed in B6C3F1 embryos developed in vitro and KM embryos developed in vivo. But for 2-cell blocked KM embryos, TRC was still not detectable even at 38 h post hCG. These evidences suggest that 2-cell-blocked embryos do initiate ZGA, and that 2-cell block phenomenon is due not to the disability in initiating ZGA, but to a delay of ZGA.

Animals↗
PubMed2003

Mitosin/CENP-F is a conserved kinetochore protein subjected to cytoplasmic dynein-mediated poleward transport.

Mitosin/CENP-F is a human nuclear protein transiently associated with the outer kinetochore plate in M phase and is involved in M phase progression. LEK1 and CMF1, which are its murine and chicken orthologs, however, are implicated in muscle differentiation and reportedly not distributed at kinetochores. We therefore conducted several assays to clarify this issue. The typical centromere staining patterns were observed in mitotic cells from both human primary culture and murine, canine, and mink cell lines. A C-terminal portion of LEK1 also conferred centromere localization. Our analysis further suggests conserved kinetochore localization of mammalian mitosin orthologs. Moreover, mitosin was associated preferentially with kinetochores of unaligned chromosomes. It was also constantly transported from kinetochores to spindle poles by cytoplasmic dynein. These properties resemble those of other kinetochore proteins important for the spindle checkpoint, thus implying a role of mitosin in this checkpoint. Therefore, mitosin family may serve as multifunctional proteins involved in both mitosis and differentiation.

Amino Acid Sequence↗
PubMed2003

[Study on the effect of H2O2 against Acanthamoeba in vitro].

OBJECTIVE: To detect the effect of H2O2 on Acanthamoeba spp.. METHODS: By Wright's stain, quantitative culture, MTT assay and lactate dehydrogenase(LDH) assessment, the influence of H2O2 on the morphological feature, proliferation speed and the survival rate of Acanthamoeba was tested. RESULTS: At low concentration of 0.125%, H2O2 can force the Acanthamoeba trophozoites into cysts irreversibly, and inhibit its proliferation. 1% H2O2 can directly destroy Acanthamoeba trophozoites. CONCLUSION: H2O2 is effective in destroying Acanthamoeba. It is possible to be used as an ideal reagent for the prevention of Acanthamoeba keratitis.

Acanthamoeba↗
PubMed2002

Rotation number, stochastic resonance, and synchronization of coupled systems without periodic driving.

This article investigates the influence of noise in a two-dimensional square array of coupled nonlinear oscillators without periodic driving. Array enhanced stochastic resonance under global as well as local noise perturbation is shown to exist under a crucial condition: the value of the rotation number of the deterministic system being zero. Meanwhile, the stochastic synchronization phenomenon is displayed in a wide range of noise intensity whether noise is added globally or locally. Furthermore, for every oscillator, the peak frequency is shown to agree with the rotation number much better than in the uncoupled system.

Journal Article↗