Biomedical subjects

Ming Zhong

Publications and source records attributed to Ming Zhong.

At least 19 recordsLinked to original sources

PubMed2026

Long-read sequencing reveals widespread novel splicing and neojunction-derived neoantigens in nasopharyngeal carcinoma.

The widespread transcriptomic diversity driven by alternative splicing (AS) contributes to all hallmarks of cancer and represents a critical source of neoantigens for personalized immunotherapy. However, unlike other major malignancies, the full repertoire of AS in nasopharyngeal carcinoma (NPC) remains underexplored. Here, we employ long-read sequencing (LR-seq) to generate a high-resolution, isoform-level transcriptomic atlas from a cohort of 14 NPC tumor samples and four immortalized nasopharyngeal epithelial cell lines. We identify a substantial number of full-length novel transcripts (22,687; ∼44.38%), which reveal diverse splicing patterns and previously unannotated splicing events. By integrating short-read RNA-seq data to quantify isoform expression, we discover a subset of novel transcripts that are differentially expressed between tumor samples and immortalized nasopharyngeal epithelial cell lines. Furthermore, LR-seq enables precise identification of chimeric readthrough fusion transcripts, such as CLDN15-FIS1 and FOXRED2-TXN2 Finally, we develop a computational framework, tumor-specific splicing neoantigen detection (TS-SNAD), to predict neoantigens originating from novel exon-exon junctions (neojunctions) in tumor-specific novel transcripts. Using this framework, we identify neojunction-derived neoantigens and experimentally validate the immunogenicity of selected HLA-B*40:01-restricted neoantigens. These neojunction-derived peptides constitute a new class of noncanonical neoantigens with significant potential for developing personalized cancer vaccines for NPC.

Humans↗

PubMed2026

Comprehensive analysis suggests CRIF1 is a potential target in breast cancer associated with prognosis and immune infiltration.

BACKGROUND: CRIF1 is a multifunctional factor that regulates cell biological processes such as the cell cycle, cell proliferation, and energy metabolism, and it is a new molecule that contributes to the poor prognosis of many malignancies. However, its involvement in breast cancer development is not fully known. MATERIALS AND METHODS: To investigate the relationship between CRIF1 expression, prognosis, and clinical characteristics using The Cancer Genome Atlas (TCGA-BRCA). The relationship between CRIF1 expression and the immunological microenvironment was investigated using CIBERSORT, ESTIMATE. Breast tissue and CRIF1 expression were validated by IHC. A tiny interfering plasmid was designed to transiently transfect breast cancer cell lines, and proliferation-related functional tests were carried out. The effect of sh CRIF1 on tumor formation was confirmed using a subcutaneous tumor experiment in naked mice. RESULTS: We discovered that CRIF1 was highly elevated in breast cancer tissues and associated with a poor prognosis. CRIF1 stimulates breast cancer cell proliferation, migration, and invasion. Knockdown decreased PI3K/AKT/mTOR signaling, which boosted autophagy activity. Immune infiltration research revealed that patients with high CRIF1 expression had higher CD8+ T cell expression but reduced macrophage M2 expression. CONCLUSION: Upregulation of CRIF1 in breast cancer cells enhances malignant behavior, which may be mediated by PI3K/AKT/mTOR signaling and is linked to cellular autophagy.

Humans↗

PubMed2007

Diagnostic value of CTA and MRA in intracranial traumatic aneurysms.

OBJECTIVE: To investigate the diagnostic value of computerized tomographic angiography (CTA) and magnetic resonance angiography (MRA) for intracranial traumatic aneurysms (TAs). METHODS: CTA and MRA of six patients with intracranial TAs verified by digital subtraction angiography (DSA) and surgery were retrospectively analysed. All patients were examined by nonenhanced computerized tomography (CT) and two by CTA. The source data were reconstructed by volume rendering (VR) and multi-planar reconstruction (MPR) from CTA. Four of them had maximum intensity project (MIP) from MRA. RESULTS: Of the six patients, a total of seven TAs were detected by CTA and MRA examinations. Five cases had only one TA and one case had two TAs. The average diameter was 2.3 cm (1.1-3.3 cm). CTA demonstrated two TAs appeared at the cavernous segment of the internal carotid artery (ICA) and the middle cerebral artery (MCA) respectively. MCA TA was definitely and clearly demonstrated on VR images, whereas VR images failed to depict the cavernous ICA TA, which was detected on MPR images. Two TAs were found irregular saccular shape, irregular margin of parent artery and wide neck on CTA. Four MRA examinations demonstrated five TAs, including the cavernous segment ICA TAs (2 cases), the supraclinoid segment ICA TA (1 case), and the cavernous segment associated with opposite side of the petrosal segment ICA TA (1 case). In a cavernous ICA TA, MRA only revealed aneurysm body, whereas aneurysm neck and distal segment of the parent artery were not revealed. In the remaining cases, MRA clearly depicted aneurysm body and parent artery, whereas the neck was not displayed. ICA TAs showed irregular capsule-like high signal intensity on MRA images. Four TAs exhibited irregular distal segment of the parent artery. TAs at the supraclinoid segment or MCA failed to find fracture signs on nonenhanced CT. CONCLUSIONS: Both CTA and MRA examinations are the effective non-invasive method of imageology for diagnosing intracranial TAs, while CTA is more eligible for diagnosing TAs after nonenhanced CT has demonstrated skull base fractures.

Adult↗

PubMed2006

Determination of phthalates in water samples using polyaniline-based solid-phase microextraction coupled with gas chromatography.

A simple solid-phase microextraction (SPME) device, coupled with gas chromatography-flame ionization detection (GC-FID), was developed to detect trace levels of phthalates in environmental water samples. Polyaniline (PANI) was chosen as the sorbent for the SPME device and was electrochemically deposited on a stainless steel wire to achieve high thermal and mechanical stability. The porous structure of the PANI film, characterized by scanning electron microscopy (SEM), suggested large extraction capability. Key parameters were optimized and five phthalates were selected to evaluate the SPME-GC procedures. The method was also applied to the analysis of lake and river water samples. Control experiments were carried out using commercial polyacrylate (PA) fiber. The new PANI-SPME-GC method offers high accuracy, precision and sensitivity and low detection limits. Thus, the method developed could be used as a new way to monitor the trace levels of phthalates in water medium. A possible extraction mechanism was investigated using electrochemical impedance spectroscopy (EIS).

Acrylates↗

PubMed2006

Changes in connexin 43, metalloproteinase and tissue inhibitor of metalloproteinase during tachycardia-induced cardiomyopathy in dogs.

OBJECTIVE: To study changes in connexin, metalloproteinase and tissue inhibitor of metalloproteinase levels during tachycardia-induced cardiomyopathy (TIC). METHODS: Canine models of TIC were established by rapid right atrial pacing at 350-400 beats per min for 8 weeks in 11 dogs, six dogs acted as a sham operation group. Echocardiography, left ventricular pressure and its first derivation with time (positive and negative maximum, dp/dtmax, -dp/dtmax), and intracardiac electrograms were recorded before and after rapid pacing at 1, 4 and 8 weeks. Data were acquired in sinus rhythm. Ultrastructural changes in left ventricular tissue were observed by transmission electron microscope. Connexin 43 (Cx43) levels in the left ventricular myocardium were measured by confocal laser microscopy. The relative abundance of matrix metalloproteinase (MMP-2) and tissue inhibitor of metalloproteinase (TIMP-2) were studied by immunoblotting. RESULT AND CONCLUSIONS: (1) Ventricular dilatation and systolic dysfunction occurred after 1 week of rapid right atrial pacing. (2) There was structural damage to the myofibrils, mitochondria, and the sarcoplasmic reticulum with intercalated disk discontinuity. (3) Levels of Cx43 decreased significantly and gap junction remodelling occurred during TIC. (4) TIC may result from several mechanisms, such as ultrastructural changes or gap junction and matrix remodelling.

Animals↗

PubMed2006

Changes in metalloproteinase and tissue inhibitor of metalloproteinase during tachycardia-induced cardiomyopathy by rapid atrial pacing in dogs.

BACKGROUND: It was the aim of this study to investigate the variation in metalloproteinase and tissue inhibitor of metalloproteinase (TIMP) connexin levels during tachycardia-induced cardiomyopathy (TIC). METHODS: Canine models of TIC were established by rapid right atrial pacing at 350-400 beats per min for 8 weeks in 11 dogs, with another 6 dogs acting as sham operation group. Echocardiography, left ventricular pressure and its first derivation with time (positive and negative maximum, dp/dt(max) and -dp/dt(max)), as well as intracardiac electrograms were recorded before and after rapid pacing at 1, 4 and 8 weeks. Data were acquired in sinus rhythm. Ultrastructural changes in left ventricular tissue were observed by transmission electron microscope. The relative abundance of matrix metalloproteinase (MMP)-9 and TIMP-1 was studied by immunoblotting. RESULTS: The following hemodynamic changes were detected after 8 weeks of rapid pacing: the TIC group had decreased dp/dt(max) (p < 0.05), the left ventricular relaxation time constant (tau) was prolonged (p < 0.05), both left ventricular end-diastolic volume and left ventricular end-systolic volume were decreased (p <0.05), left ventricular end-diastolic pressure was significantly increased (p <0.05), and -dp/dt(max) was significantly decreased (p <0.001) compared with the control group; no statistical differences in the left ventricular ejection fraction between weeks 1, 4 or 8 (p >0.05) were observed, but left ventricular ejection fraction was significantly decreased after 1 week of pacing (p < 0.05). The left ventricular end-diastolic volume was increased after 1 week of pacing compared with the control group (24.15 +/- 8.15 vs.11.19 +/- 4.41 ml; p <0.05), as shown by echocardiography. Compared with the control group, MMP-9 was significantly higher (0.217 +/- 2.16 E-02 vs. 0.314 +/- 5.263 E-02; p < 0.001), while TIMP-1 was decreased (0.230 +/- 8.944 E-02 vs. 0.120 +/- 9.258 E-03; p < 0.001). CONCLUSIONS: Ventricular dilatation and systolic dysfunction occurred after 1 week of rapid right atrial pacing. Enlarged and disarrayed fibers and mitochondria with disintegrated crystal and an anarchic pattern were observed. Additionally, moderate dilation of the rough endoplasmic reticulum and intercalated disk discontinuity were seen after 8 weeks of pacing, and MMP-9 was increased and TIMP-1 was decreased after the same time period.

Animals↗

PubMed2006

[Mechanism of reversion of myocardial interstitial fibrosis in diabetic cardiomyopathy by valsartan].

OBJECTIVE: To investigate the mechanism of reversion of myocardial interstitial fibrosis in diabetic cardiomyopathy (DCM) by valsartan. METHODS: Forty male wistar rats were randomly divided into 3 groups: DCM group, n = 16, fed with high-fat diet for 4 weeks and injected intraperitoneally with streptozocin (STZ) once to induce hyperglycemia so as to construct a DCM model, and then perfused into the stomach with normal saline; valsartan group, n = 16, to be constructed into DCM model and then perfused into the stomach with valsartan once daily; and control group (n = 8, fed with normal food and perfused into the stomach with normal saline. Four weeks after feeding (i.e., before injection of STZ), 1 week after STZ injection, and by the end of experiment after 12-hour fasting samples of venous blood were collected to detect the contents of triglyceride and fasting blood-glucose and insulin; by the end of experiment miniature cardiac catheter was inserted into the left ventricle to conduct hemodynamic examination. Then myocardium tissues were collected, collagen content was detected by Masson staining, real-time RT-PCR was used to detect the mRNA expression of thrombospondin (TSP)-1 and tumor growth factor (TGF)-beta(1) mRNA, expression, and Western blotting was used to detect the protein expression of TSP-1, active TGF-beta(1) (A-TGF-beta(1)) and latent TGF-beta(1) (L-TGF-beta(1)). RESULTS: By the end of the experiment, the body weights, and insulin sensitivity index were significantly lower and fasting blood-glucose, and serum triglyceride and cholesterol were significantly higher in the DCM group and valsartan group in comparison with those in the control group (all P < 0.01), however, there was no significant differences in fasting insulin among these 3 groups. The values of left ventricular systolic pressure (LVSP) and +/- dp/dt(max) were significantly lower and left ventricular end diastolic pressure were significantly higher in the DCM group in comparison with the control group (all P < 0.01). The LVSP and -dp/dt(max) were significantly higher and LVEDP was significantly lower in the valsartan group than in the DCM group (all P < 0.05). The LVEDP was significantly higher and -dp/dt(max) was significantly lower in the valsartan group than in the control group. Electron microscopy showed the distribution of a great amount of collagen in the myocardial interstitial tissue. The collagen content of the DCM group was 17 +/- 3, significantly higher than that of the control group (11 +/- 3, P < 0.05), and the collagen content of the valsartan group was 13 +/- 3, significantly lower than that of the DCM group (P < 0.05). The mRNA expression of TSP-1 and that of TGF-beta(1) were significant higher in the DCM group than in the control group (both P < 0.05), and were significantly lower in the valsartan group than in the DCM group (both P < 0.05); however, the TGF-beta(1) mRNA expression in the valsartan group was significantly higher in the valsartan group than in the control group (P < 0.05). The values of protein expression of TSP-1, A-TGF-beta(1) and L-TGF-beta(1) were all significantly higher in the DCM group than in the control group (all P < 0.05), and the values of protein expression of TSP-1 and A-TGF-beta(1) in the valsartan group were both significantly lower than those in the DCM group (both P < 0.05), however, there was no significant difference in the protein expression of L-TGF-beta(1) between the valsartan group and DCM group. CONCLUSION: Valsartan amelioorates myocardial interstitial fibrosis in DCM via TSP-1/TGF-beta(1) signaling pathway.

Animals↗

PubMed2006

[Effects of genistein on the expressions of cell cycle proteins in salivary adenoid cystic carcinoma cell line SACC-83].

PURPOSE: To investigate the molecular mechanism of cell cycle arrest induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83. METHODS: SACC-83 cells cultured in vitro were treated with genistein, the expressions of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.5 software. RESULTS: With the increase of concentration of genistein and the elongation of time, the expression of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins was significantly decreased. Treated with 220 micromol/L of genistein for 3 days, the expression level of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins was 58%, 64%, 46% and 43% of the control group, respectively (P<0.01). CONCLUSION: The cell cycle G2/M arrest induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the downregulations of CyclinB1, Cdk1, CyclinD1 and Cdk4 protein expressions.

Carcinoma, Adenoid Cystic↗

PubMed2006

[Effect of valsartan on Tribble 3 gene expression in rats with experimental diabetic cardiomyopathy].

OBJECTIVE: Tribbles, a protein family controlling mitogen-activated protein kinase cascades, might contribute to the remodeling process in dilated cardiomyopathy. We investigated the gene expression of Tribble 3 (TRB(3)), cardiac function and collagen changes in rats with diabetic cardiomyopathy (DCM) and the modulating effects of valsartan on them. METHODS: Male Wistar rats were fed with high cholesterol diet throughout the study period, streptozocin (30 mg/kg, i.p) was given at the 28th day, valsartan (30 mg.kg(-1).d(-1), n = 13) or placebo (n = 11) was administered at the 35th day to rats with fasting blood glucose > or = 11.1 mmol/L per gavage for another 12 weeks. Control rats (n = 8) were fed with regular chow. Fasting blood glucose was monitored throughout the study, left ventricular function was determined by echocardiography, myocardial collagen content quantified after Masson-staining and myocardial mRNA expression of TRB(3) detected by quantification real-time RT-PCR at the end of study. RESULTS: Cardiac function was significantly improved (EF: 74% +/- 10% vs. 66% +/- 7%, P < 0.05), myocardial collagen content decreased (13.23 +/- 3.14 vs. 16.92 +/- 3.18, P < 0.05) in rats with DCM treated with valsartan. Moreover, TRB(3) mRNA was significantly increased in rats with DCM compared to control rats (0.0198 +/- 0.0082 vs. 0.1108 +/- 0.0933, P < 0.05) and the increase could be significantly attenuated by valsartan (0.0367 +/- 0.0234, P < 0.05 vs. DCM). A significant positive correlation was observed between myocardial TRB(3) mRNA and myocardial collagen content (r = 0.67, P < 0.05) and between TRB(3) mRNA and fasting blood glucose (r = 0.69, P < 0.05) in rats with DCM. CONCLUSION: Our results show for the first time that myocardial TRB(3) mRNA is upregulated in rats with DCM and which could be down-regulated by valsartan.

Animals↗

PubMed2006

[The role of glucose/TSP-1/TGFbeta1 signal pathways in diabetic cardiomyopathy].

OBJECTIVE: Hyperglycemia could upregulate transforming growth factor-beta (TGFbeta(1)) via thrombospondin (TSP-1) and induce fibrotic renal disease in the rat in vivo and myocardial fibrosis was related to cardiac dysfunction in diabetic patients. We explored the role of glucose/TSP-1/TGFbeta(1) signal pathways in the development of diabetic cardiomyopathy (DCM). METHODS: Male Wistar rats were fed with high cholesterol diet for 17 weeks, streptozocin (30 mg/kg, i.p) was given at the 28th day, rats with fasting blood glucose > or = 11.1 mmol/L by the end of the 5th week were assigned to DCM group (n = 11). Control rats (n = 8) were fed with regular chow. Fasting blood glucose (FBG) was monitored throughout the study. After hemodynamic measurements by the end of the study, myocardial collagen content was quantified in Masson-stained samples and the mRNA expressions of TSP-1 and TGFbeta(1) were detected by quantification real-time RT-PCR. The protein levels of TSP-1, active and latent TGFbeta(1) were detected by Western blot. RESULTS: Compared with control group, cardiac function was decreased as shown by significantly reduced left ventricular systolic pressure, dp/dt(max) and dp/dt(min), while the myocardial collagen content was significantly increased in the DCM group (11.01 +/- 3.05 vs. 16.92 +/- 3.18, P < 0.01). The myocardial mRNA expressions of TSP-1, TGFbeta(1) and protein expressions of TSP-1, active and latent TGFbeta(1) in the DCM group were also significantly higher than those of the control group. Moreover, myocardial collagen was positively correlated to FBG (r = 0.746, P < 0.01); mRNA expressions of TSP-1 and TGFbeta(1), protein expressions of TSP-1 and active TGFbeta(1) were positively correlated to FBG and myocardial collagen (P < 0.05). However, there were no correlations between the protein expression of latent TGFbeta(1) and FBG and myocardial collagen. CONCLUSION: The pathway of glucose/TSP-1/TGFbeta(1) might play an important role in myocardial interstitial fibrosis of DCM. It may be the basis of novel therapeutic approaches for ameliorating DCM.

Animals↗

PubMed2006

[Expression of telomerase genes hTRTmRNA in oral squamous cell carcinomas].

PURPOSE: To investigate the expression of telomerase genes in oral squamous cell carcinomas(OSCC). METHODS: Telomerase genes hTRTmRNA in 65 cases of OSCC, 25 cases of epidermal cells with abnormal hyperplasia and 20 cases of normal oral mucosa were detected by in situ hybridization. The digoxin-labelled probe was targeted to reverse transcription domain. Routine hybirdization was carried out, oral squamous cancer was used as positive control and the negative control without probe. Data was statistically analyzed by SPSS software package for chi-square test,group t test and kendall correlation analysis. RESULTS: The expression of hTRT mRNA was weak in epithelium immediately adjacent to carcinomas and normal oral mucosa (4/20,20.0%), weaker in epidermal cells with abnormal hyperplasia (11/25,44.0%), while very strong expression (54/65,83.1%)in cases of OSCC. The hTRT mRNA expression levels between OSCC and other groups were significantly different P<0.01).The difference between normal oral mucosa, epithelium immediately adjacent to carcinomas and epidermis cells with abnormal hyperplasia was not significant (P>0.05) and the difference between epidermal cells with abnormal hyperplasia and pre-cancer alteration was not significant (P>0.05). CONCLUSION: The expression of telomerase genes (hTRT mRNA) in OSCC is closely related to the malignant transformation of oral mucosa cell. The reactivated telomerase genes (hTRT mRNA) may play a crucial role in the development of OSCC.

Carcinoma, Squamous Cell↗

PubMed2005

[Effects of matrix metalloproteinase-9 and tissue inhibitor-1 of metalloproteinase expression on atrial structural remodeling during chronic atrial fibrillation].

OBJECTIVE: To investigate the matrix metalloproteinase-9 (MMP-9) and tissue inhibitor-1 of metalloproteinase (TIMP-1) mRNA and protein expression in chronic fibrillating human atria and to evaluate the influence of MMP-9 and TIMP-1 expression on the progress of atrial structural remodeling. METHODS: Twenty-four patients with chronic atrial fibrillation (AF) and 12 patients with sinus rhythm as control group underwent transthoracic echocardiography and left atrial appendage (LAA) tissue samples were obtained from these patients during mitral/aortic valve replacement operation. MMP-9 and TIMP-1 protein expressions were detected by immunohistochemistry and their mRNA expressions were determined by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: The left atrial and right atrial diameters increased significantly in the fibrillation group in comparison with the control group (57 +/- 6 vs 45 +/- 7, 62 +/- 10 vs 51 +/- 17, P < 0.05 approximately 0.001) The expressions of MMP-9 mRNA and protein in the LAA tissue of the AF group is upregulated (0.70 +/- 0.12 vs 0.53 +/- 0.22, and 2.25 +/- 0.73 vs 1.12 +/- 0.58, P < 0.05 approximately 0.001) and the expressions of TIMP-1 mRNA and protein were downregulated significantly (0.20 +/- 0.07 vs 0.31 +/- 0.15, and 1.12 +/- 0.48 vs 1.75 +/- 0.46, P < 0.05 approximately 0.01). The MMP-9 mRNA level was positively correlated with AF duration and the left atrial diameter (P < 0.05 approximately 0.001). CONCLUSION: There is a selective downregulation of TIMP-1 expression along with the upregulation of MMP-9 in AF, which indicates that the disturbance expression of MMP/TIMP system may promote the process of atrial structural remodeling. Enhanced MMP-9 activity may be a molecular mechanism contributing to the dilation of fibrillating human atria.

Adult↗

PubMed2005

In vitro augmentation of natural killer activity and interferon-gamma production in murine spleen cells with Agaricus blazei fruiting body fractions.

Aqueous extracts of the Agaricus blazei fruiting body prepared at different temperatures were fractionated by ethanol precipitation with various ethanol concentrations. The original aqueous extracts of A. blazei failed to stimulate natural killer (NK) cell activity in murine spleen cells in vitro, but the strongest effect was observed in a 30% ethanol-soluble-50% ethanol-insoluble fraction prepared from the extract at 40 degrees C (fraction A-50). Fraction A-50 also showed the strongest augmenting effect on interferon (IFN)-gamma production. This augmentation of NK activity and IFN-gamma production by fraction A-50 was completely abrogated by a heat treatment.

Agaricus↗

PubMed2005

[Anti-proliferation effect of genistein on salivary adenoid cystic carcinoma cell line SACC-83 in vitro].

PURPOSE: To investigate the anti-proliferation effect of tyrosine protein kinase inhibitor, genistein (4'-5,7-trihydroxyisoflavone), on human salivary adenoid cystic carcinoma cell line SACC-83 in vitro, and its effect on cell cycle. METHODS: SACC-83 cells cultured in vitro were treated with genistein,cell survival rate was calculated with MTT assay, phase contrast microscope was used to observe the status of cell growth and the morphological changes in each group, and cell cycle was detected with flow cytometry. Apoptosis was detected with Annexin V/PI staining. The results were statistically analyzed by SPSS11.5 software. RESULTS: When treated with genistein of certain concentration for certain time, SACC-83 cell growths were significantly inhibited. With the increase of concentration of genistein and elongation of acting time, the inhibitory effects increased. Cells treated with genistein showed changes in morphology, cells' volume was decreased ,and suspended cells increased. Treated with 220 micromol/L genistein for 72 hours, SACC-83 cell growths were significantly inhibited, cell cycle was arrested in G2/M phase, and cell apoptosis was induced(P<0.01). CONCLUSION: Genistein inhibits growth of human salivary adenoid cystic carcinoma cell line SACC-83, arrests cell cycle in G2/M phase, and induces cell apoptosis; these suggest that tyrosine protein kinase has important effect on the neoplasia and development of salivary adenoid cystic carcinoma.

Apoptosis↗

PubMed2005

[Expression of Interleukin-8 in KB cells infected with Candida albicans].

PURPOSE: To investigate the change of interleukin-8 (IL-8) in oral epithelial cells (KB cells) infected with Candida albicans. METHODS: Two-site ELISA and RT-PCR were applied to determine the levels of IL-8 protein or mRNA expression in the KB cells induced by hyphae, yeast, killed Candida albicans and medium respectively. The unpaired Student's t test was used to analyse the data. RESULTS: Candida albicans induced the KB cells secrete more IL-8. The hyphae, yeast, killed Candida albicans and control group marks of RT-PCR were 180.23, 186.36, 143.41 and 85.57 respectively, calculated using a two-tailed test, P<0.05, was considered to be significant, but the medium group (mark 80.87) had no significant change. The tendency of the IL-8 protein secreted by KB cells increased with the time but reduced when Candida albicans was more. CONCLUSIONS: Candida albicans is responsible for the production of IL-8 secreted by oral epithelial cells which can be expected to play an important role in the infection and defection with the local oral mucosa.

Candida albicans↗

PubMed2005

[Effect of static magnetic field on bone morphogenetic protein-2 in periodontal membrane of experimental periodontitis rat].

OBJECTIVE: To evaluate the effect of static magnetic field on expression of bone morphogenetic protein-2(BMP-2) in the periodontal membrane of experimental periodontitis rat. METHODS: Experimental periodontitis of rat was formed by ligaturing the neck of rat teeth and feeding sugar of high concentration. The magnet which the intensity of magnetic field was 0.12 tesla was put into their cheeks. The rats were sacrificed at 2nd day, 4th day and 7th day, respectively. Immuno-histochemical techniques were used to evaluate the change of BMP-2 expression. RESULTS: BMP-2 was mainly found in the plasma of fibroblast, osteoblast, cementocyte and odontoblast separately. There was not clearly difference between the periodontitis group and the normal group, but BMP-2 in the experimental group treated with static magnetic field was higher than the two groups. CONCLUSION: Static magnetic field plays an important role in repairing and remodeling of periodontitis.

Animals↗

PubMed2005

[Expression of p21WAF1, p27KIP1 and cyclin E in ameloblastoma].

OBJECTIVE: To investigate the expression of cyclin E mRNA, p21(WAF1) mRNA and p27(KIP1) protein in human ameloblastoma (AB), and to explore the clinical and biological characteristics of AB. METHODS: The expression of cyclin E mRNA, p21(WAF1) mRNA and p27(KIP1) protein in 54 cases of human AB were detected by in situ hybridization or immunohistochemistry (SP method). RESULTS: The positive expression rate of cyclin E mRNA in the cytoplasm or cell nucleus of AB was 66.7% (36/54). The expression of cyclin E mRNA increased with AB recurrence and malignant transformation, and the difference of expression among primary AB, recurrent AB, and malignant AB, was statistically significant. The positive expression ratio of cyclin E mRNA in OKC was 50.0% (8/16). The p21(WAF1) mRNA expression in the cytoplasm or cell nucleus of AB decreased, and the positive ratio was 22.6% (12/54) in AB, 37.5% (6/16) in OKC, respectively. The p27(KIP1) protein expression in the cell nucleus of AB was positive in a small number of cases, and the positive rate was 16.7% (9/54) in AB, 6.3% (1/16) in OKC, respectively. CONCLUSIONS: The genesis and invasion of AB is associated with the cell proliferation and differentiation, and regulated by the higher expression of cyclin E and the lower expression of p21(WAF1) and p27(KIP1).

Adolescent↗

PubMed2005

[Effect of bite force lost on the expression of iNOS in the rat periodontium].

PURPOSE: To investigate the molecular mechanism of the effect of bite force loss on periodontium remodeling. METHODS: For establishing a rat model of different bite forces for the study, the left maxillary molars of Wistar rats were extracted and the left mandibular molar area was used as the model of the bite force lost. The animals were sacrificed after 6 hours,1,2,3 days and 1,2,3,4weeks. The prepared tissue specimens were processed for the study of the change in the histologic morphology and the expression of iNOS protein in PDLC and osteoblasts with HE staining, immunohistochemistry and RT-PCR techniques, and the results were analyzed by one-way ANOVA,Dunnet t test and paired samples test. RESULTS: In the group of lost biting force, the derangement of periodontal ligament and resorption of alveolar bone were observed in histologic study: the structure of periodontal ligament deranged ,fibers and cells arrayed abnormally, there were many holes in the alveolar bone, even osteoclasts were present; the expression of iNOS was observed dramatically changed: the expression of iNOS in periodontal ligament fibroblasts and osteoblasts was higher than normal, especially at 2 days and 3 weeks,so as the results of RT-PCR.There was a significant correlation between bite force lost and the increased expression of iNOSmRNA. CONCLUSIONS: Bite force lost significantly increased the expression of iNOSmRNA in PDLC and osteoblasts, which suggests that iNOS may play an important role in the process of periodontium remodeling.

Alveolar Bone Loss↗