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Mitsuo Ochi

Publications and source records attributed to Mitsuo Ochi.

At least 37 records · Page 2Linked to original sources
PubMed2006

Inhibitory effects of a new bisphosphonate, minodronate, on proliferation and invasion of a variety of malignant bone tumor cells.

Little is known about the biological effects of bisphosphonates on primary malignant bone tumors. The purpose of this study was to investigate the antitumor effects of newly developed minodronate (MIN) on a variety of human malignant bone tumors. We examined the effects of MIN and clinically relevant incadronate (INC) on the proliferation, apoptosis, and cell cycle of two osteosarcoma (Saos-2, MG-63), two chondrosarcoma (SW1353, OUMS27), and two Ewing's sarcoma (RD-ES, SK-ES-1) cell lines. Furthermore, we investigated the anti-invasion effects of MIN on sarcoma cells and the effects of MIN on tumor growth in nude mice. MIN inhibited the viability of all six cell lines in a dose-dependent manner with IC50 values of 2.7 to 5.0 microM, which were significantly lower than those of INC. Importantly, both bisphosphonates affected the viability of normal bone marrow stromal cells much less than sarcoma cells. Both bisphosphonates induced cell cycle perturbation in all sarcoma cells tested and apoptosis in Saos-2 and SW1353 cells, although they failed to induce apoptosis in RD-ES and SK-ES-1 cells. MIN significantly suppressed invasion, even at a low concentration of 1 microM (p < 0.01). Daily injection of 5 microg of MIN inhibited the growth of SK-ES-1 xenograft sarcoma in nude mice without loss of body weight. These findings suggest that MIN may have a beneficial adjuvant role in the treatment of patients with malignant bone tumors.

Animals↗
PubMed2006

The autotomy relief effect of a silicone tube covering the proximal nerve stump.

A technique of covering the proximal nerve stump (PNS) has been reported as a preventative method or treatment for neuroma. However, its detailed pain relief mechanism remains unknown. We created a silicone tube model in which the PNS of the rat sciatic nerve was introduced into the tube, whereas the controls had no tube. The score of autotomy observed in the tube group was lower than that in the control group at 3 days to 2 weeks after surgery, which suggested that the silicone tube had pain-like behavior inhibitory action. To elucidate the mechanism of autotomy inhibition, immunohistochemistry and Toluidine blue staining were performed in the PNS. The increase in S-100-immunoreactivity (IR) including Schwann cells was inhibited at 1 week after surgery in the tube group, and the increase in the number of macrophages shown by ED-1-IR at 1, 2, and 4 weeks after surgery was similarly inhibited. Toluidine blue staining showed that the increase in the number of mast cells was inhibited at 1, 2, and 4 weeks after surgery and in the number of lymphocytes at 1 and 2 weeks after surgery in the tube group. Therefore, blocking of the infiltration of inflammatory cells into the PNS by the silicone tube was thought to be the mechanism of autotomy inhibition. To further explore details of this mechanism, the expression of nerve growth factor (NGF), production of which is induced by inflammatory cells and of the NGF receptor TrkA was examined in the PNS and the dorsal root ganglion using immunohistochemistry and a ribonuclease protection assay. In the PNS, the increase in NGF-IR was inhibited at 1, 2, and 4 weeks after surgery in the tube group, suggesting that this could be one of the pain-like behavior inhibitory effects.

Animals↗
PubMed2006

Anterior cruciate ligament augmentation procedure with a 1-incision technique: anteromedial bundle or posterolateral bundle reconstruction.

Arthroscopic examination before anterior cruciate ligament (ACL) reconstruction sometimes reveals that there is a relatively thick ACL remnant bridging the femur and the tibia that may function to stabilize the knee, although its attachment is not the same as the anatomic site. We have established an ACL augmentation procedure that preserves the ACL remnant with the 1-incision technique using an autogenous semitendinosus tendon and EndoButton-CL (Acufex, Smith & Nephew, Mansfield, MA) instead of ACL reconstruction. When the ACL remnant was attached to the anteroinferior portion of the anatomic femoral origin and the posterolateral (PL) bundle was well preserved, the anteromedial (AM) bundle was reconstructed. When, in contrast, the ACL remnant was attached to the high-noon position with a well-preserved AM bundle, the PL bundle should be reconstructed. We have performed 13 AM bundle reconstructions and 4 PL bundle reconstructions. The merits of our ACL augmentation procedure are (1) it is performed under arthroscopy with 1 incision, (2) it avoids resection of the ACL remnant, which contributes to prevention of anterior laxity, (3) it preserves the ACL remnant's neural elements and mechanoreceptors, and (4) it provides a favorable influence on vascularity and reinnervation to the graft.

Anterior Cruciate Ligament↗
PubMed2006

Lateral compartment osteoarthritis of the knee after meniscectomy treated by the transplantation of tissue-engineered cartilage and osteochondral plug.

Management of osteoarthritis of the knee after meniscectomy has been challenging, especially for young patients, because articular cartilage has very poor healing capacity because of its lack of vessels, nerve supply, and isolation from systemic regulation. Osteoarthritic lesions often involve both femoral and tibial cartilage, requiring treatments for both lesions. We report the case of a 14-year-old girl with lateral compartment osteoarthritis of the knee after a total meniscectomy of the discoid meniscus, who was successfully treated by the transplantation of both tissue-engineered cartilage made ex vivo for a femoral lesion and an autologous osteochondral plug for a tibial lesion. We treated both femoral and tibial cartilage defects simultaneously with this procedure. We confirmed cartilaginous regeneration in both femoral and tibial lesions at second-look arthroscopy. This procedure is one option to prevent further development of osteoarthritis in young patients.

Adolescent↗
PubMed2006

Predictive value of preoperative 3-dimensional computer tomography measurement of semitendinosus tendon harvested for anterior cruciate ligament reconstruction.

PURPOSE: The aim of this study was to evaluate preoperative measurement of the semitendinosus tendon (ST) by 3-dimensional computed tomography (3-D CT), before using ST as the substitute in the anterior cruciate ligament (ACL) reconstruction. TYPE OF STUDY: Cross-sectional study to compare anatomic findings with clinical findings. METHODS: The study involved 28 patients who underwent ACL reconstruction using autogenous ST graft. Preoperative 3-D CT images of ST were obtained with a multidetector-row CT scanner, and rendered qualitatively by the volume-rendering technique. The length and cross-sectional area of ST measured with 3-D CT were compared with the length of the harvested ST and the cross-sectional area of the grafted quadrupled tendon. RESULTS: The total length of ST measured with 3-D CT ranged from 220.3 to 285.4 mm, with a mean (+/- SD) of 248.0 +/- 17.1 mm, and that measured at surgery ranged from 230.0 to 290.0 mm, with a mean of 257.1 +/- 16.5 mm. These 2 values showed a close positive correlation (gamma = 0.634, P = .002). The cross-sectional area of ST measured with 3-D CT ranged from 4.29 to 18.5 mm2 with a mean of 11.9 +/- 4.6 mm2, and that of the substitute measured at surgery ranged from 28.3 to 56.7 mm2 with a mean of 45.5 +/- 6.9 mm2). There was no significant correlation between the areas of ST measured by CT and the areas of ST measured during surgery. CONCLUSIONS: Preoperative 3-D CT imaging predicts the length of ST. These findings support the measurement of ST length by 3-D CT as a useful tool for planning ACL reconstructive surgery and for providing important preoperative information to patients. LEVEL OF EVIDENCE: Level III, diagnostic study.

Adolescent↗
PubMed2006

New hamstring fixation technique for medial collateral ligament or posterolateral corner reconstruction using the mosaicplasty system.

In this study, we describe a new hamstring fixation technique with a bone graft using the Mosaicplasty system for the reconstruction of the medial collateral ligament (MCL) or posterolateral corner (PLC) of the knee. In this technique, a cylindrical bone plug (6.5 mm in diameter, 15 mm in length) is harvested from the anatomical femoral attachment of the MCL or popliteal tendon using the Mosaicplasty system. After placing the autologous semitendinosus or gracilis tendon as a graft in the created bone socket, the bone plug is replaced over the graft. The graft is secured with a spike staple after appropriate tension is applied to the graft. This fixation technique can provide rigid and less invasive fixation, because the distance between the fixation sites can be shorter than that in the Endobutton technique. Compared to fixation with only a staple with no bone graft, the bone graft may enhance tendon to bone healing.

Arthroplasty↗
PubMed2006

Reconstruction of lateral ligament with arthroscopic drilling for treatment of early-stage osteoarthritis in unstable ankles.

PURPOSE: The purpose of this study was to investigate the clinical, radiologic, and arthroscopic results of lateral stabilization with reconstruction of the lateral ligaments and cartilage regeneration with arthroscopic drilling for the treatment of moderate osteoarthritis of the ankle with simultaneous lateral instability of the ankle (LIA). METHODS: There were 16 cases with LIA, 7 of which had stage 2 osteoarthritis of the ankle according to the radiographic classification of Takakura et al. and 9 of which had stage 3 osteoarthritis. Arthroscopic drilling was performed with a motorized drill for the chondral defect, and anatomic reconstruction of the lateral ligament with an autologous gracilis tendon graft was performed for LIA. Clinical, radiologic, and arthroscopic evaluations were performed before surgery and at the most recent follow-up. RESULTS: In stage 2 cases the mean score on the American Orthopaedic Foot and Ankle Society ankle-hindfoot scale was 42.5 +/- 6.8 points before surgery and 87.4 +/- 4.2 points at the most recent follow-up (P = .0002). In stage 3 cases the mean score was 41.4 +/- 8.0 points before surgery and 61.2 +/- 7.4 points at the most recent follow-up (P = .0001). The talar tilt angles on standard stress radiography in stage 2 cases were 17.4 degrees +/- 4.5 degrees before surgery and 3.4 degrees +/- 0.9 degrees at the most recent follow-up (P = .0009). In stage 3 cases the mean talar tilt angles were 18.2 degrees +/- 4.7 degrees before surgery and 3.6 degrees +/- 0.7 degrees at the most recent follow-up (P < .0001). In all cases the radiographic classification of Takakura et al. was unchanged between assessments before surgery and at the most recent follow-up. With regard to arthroscopic findings at 1 year after surgery, the International Cartilage Repair Society's cartilage repair assessment score showed 6 nearly normal and 1 abnormal stage 2 cases and 1 abnormal and 8 severely abnormal stage 3 cases. CONCLUSIONS: Reconstruction of the lateral ligament with arthroscopic drilling as a surgical procedure for the treatment of stage 2 osteoarthritis with LIA can be recommended. LEVEL OF EVIDENCE: Level IV, therapeutic case series.

Adult↗
PubMed2006

Pollicization of the index finger for traumatic thumb amputation.

BACKGROUND: Indications for pollicization of the index finger have decreased since the toe-to-thumb transfer and wraparound flap have been developed for thumb reconstruction. Pollicization, however, remains a useful method of thumb reconstruction depending on the amputation level, age, and patients' requests. The purpose of this study was to evaluate the authors' results and to clarify indications for pollicization. METHODS: Eight patients treated by pollicization of the index finger after thumb amputation were reviewed retrospectively. Five men and three women were included. The age at surgery ranged from 24 to 66 years, with a mean age of 43 years. Amputation levels included the metacarpophalangeal joint in four patients, the first metacarpal in three, and the carpometacarpal joint in one. The period between injury and pollicization ranged from 0 days to 4 years. Postoperative evaluations included thumb range of motion, opposition and pinch function, grasp and pinch strength, sensation, conversion of position sense, a "picking-up" test, and appearance. RESULTS: The follow-up period ranged from 5 to 28 years, with an average of 15 years, excluding one patient who died during the follow-up period. The thenar muscles were retained in four patients, who had excellent postoperative function and satisfactory results. CONCLUSIONS: Pollicization gives excellent results if the thenar muscles can be retained; opposition can be expected after this procedure. However, if the thenar muscles cannot be preserved, the reconstructed thumb may function as a post against the fingers to regain grasping and pinching functions.

Adult↗
PubMed2006

An alternative for descending thoracic aortic aneurysm repair.

A simple technique termed "clamp test and spinal cord-plegia" for descending thoracic aortic aneurysm surgery is described. The clamp test is a passive method of creating ischemic conditions, whereas spinal cord-plegia is an active method of decreasing metabolism. This technique is a practical test that double-checks for critical feeding arteries and seems to have an excellent spinal cord-preserving effect.

Aged↗
PubMed2006

Superior retinaculoplasty for recurrent dislocation of peroneal tendons.

BACKGROUND: Although dislocation of peroneal tendons is an uncommon injury, if it does occur, daily and sports activities may be restricted because of snapping of the tendons and pain in the posterolateral aspect of the ankle joint. Many operative procedures have been reported for this pathology, including anatomic reattachment of the retinaculum, bone block procedures, tissue-transfer procedures, and groove-deepening. However, no procedure has been established as the "gold standard." Successful results were reported after anatomic reattachment of the detached superior peroneal retinaculum and periosteum of the fibula. We have modified the procedure to reduce operative invasiveness and introduced a method to determine proper tension when suturing the retinaculum to the fibula. METHODS: In our retinaculoplasty, the false pouch was opened through one incision, and the retinaculum was sutured to the fibula while measuring the tension, avoiding too tight or too loose suturing. We performed this procedure in 20 patients with symptomatic dislocation of peroneal tendons in whom conservative treatment had failed. We followed them over 2 years, and clinical results were evaluated. RESULTS: No patient had re-dislocation of the peroneal tendons during the followup period. Fifteen of 18 patients who had been involved in sports activities returned to their previous activities without reducing their activity levels. All patients acquired full range of motion of the ankle joint postoperatively. The preoperative American Orthopaedic Foot and Ankle Society (AOFAS) scores improved significantly (p value < 0.01) postoperatively. CONCLUSIONS: This study demonstrated that the described repair successfully treated patients with symptomatic dislocation of the peroneal tendon and should be considered for this pathology.

Ankle↗
PubMed2006

Autologous bone marrow mononuclear cell implantation induces angiogenesis and bone regeneration in a patient with compartment syndrome.

A 28-year-old man developed compartment syndrome in the right lower leg after fracture of the tibia and fibula. Despite fasciotomy, many arteries collapsed and union of the tibial and fibula fractures did not occur. Autologous bone marrow mononuclear cell (BMMNC) implantation for therapeutic angiogenesis and subsequent bone regeneration was performed and 4 weeks later, angiography showed a marked increase in collateral vessels surrounding the tibial fracture, and union was completed 6 months later. BMMNC implantation therapy might provide therapeutic angiogenesis and osteogensis in patients with compartment syndrome.

Bone Marrow Transplantation↗
PubMed2006

Rotational acetabular osteotomy for advanced osteoarthritis secondary to dysplasia of the hip.

BACKGROUND: Satisfactory intermediate and long-term results of rotational acetabular osteotomy for the treatment of early osteoarthritis secondary to developmental dysplasia of the hip have been reported. The purpose of this study was to examine the results of rotational acetabular osteotomy in patients with advanced osteoarthritis secondary to developmental dysplasia of the hip. METHODS: We performed a retrospective review of the results of rotational acetabular osteotomy in forty-three patients (forty-three hips). All of the patients had radiographic evidence of advanced-stage osteoarthritis, defined as narrowing of the joint space with cystic radiolucencies and small osteophytes according to the staging system of the Japanese Orthopaedic Association. Forty-one patients were female, and two were male. The mean age was 43.8 years at the time of surgery, and the mean duration of follow-up was 8.5 years. Clinical follow-up was performed with use of the system of Merle d'Aubigné and Postel. The center-edge angle, acetabular roof angle, head lateralization index, and minimum width of the joint space were measured on radiographs made preoperatively, postoperatively, and at the time of final follow-up. Postoperative joint congruency was classified into four grades. RESULTS: The mean preoperative Merle d'Aubigné clinical score was 13.3 points, which improved to a mean of 15.4 points at the time of the latest follow-up (p < 0.0001). The mean center-edge angle improved from 0.7 degrees preoperatively to 29 degrees at three months postoperatively (p < 0.0001), the mean acetabular roof angle improved from 30 degrees to 11 degrees (p < 0.0001), the mean head lateralization index improved from 0.69 to 0.65 (p < 0.01), and the mean minimum width of the joint space improved from 2.2 to 2.5 mm (p < 0.0003). Ten hips had radiographic evidence of progression of osteoarthritis. Kaplan-Meier survivorship analysis, with radiographic signs of progression of osteoarthritis as the end point, predicted a ten-year survival rate of 72.2%. CONCLUSIONS: Rotational acetabular osteotomy for advanced osteoarthritis secondary to dysplasia of the hip in properly selected patients can improve clinical scores and is associated with a lack of radiographic signs of progression of osteoarthritis in most patients. LEVEL OF EVIDENCE: Therapeutic Level IV.

Acetabulum↗
PubMed2005

Differential activation of astrocytes and microglia after spinal cord injury in the fetal rat.

BACKGROUND: As the immature spinal cord was nerve growth permissive, we examined glial reactions that influence regeneration of the spinal cord in a fetal rat spinal cord injury model. METHODS: Three, 7, 21, and 35 days after intrauterine surgery, offspring were killed and the thoracic and lumbar spinal cords were carefully removed from the spinal column and then cut into 10 mum longitudinal sections. These sections were stained with hematoxylin-eosin, anti-glial fibrillary acidic protein antibody (GFAP) as a marker of astrocytes, and anti-complement CR3 antibody (OX-42) as a marker of microglia. A cordotomy model in a young adult rat was utilized as a control. RESULTS: In the present study, collagen fibers and scar formation were seen in the severed spinal cords of mature rats, but scar formation was not seen in the fetal rat cordotomy group, regardless of spinal continuity. In the control group, biological activity of GFAP-positive cells increased over time. In the fetal rat cordotomy model, activity elevated slightly immediately after cordotomy, and disappeared shortly thereafter. In the control group, OX-42-positive macrophage-like cells proliferated over time. However, in the fetal rat cordotomy model, OX-42- positive macrophage-like cells were recognized on postoperative days 3 and 7, and then disappeared. At 5 mm from the cordotomy site, reactive microglia were recognized in the white matter of control group spinal cords, but these microglia were not recognized in the fetal rat cordotomy model. CONCLUSIONS: In the present study, collagen fibers and scar formation were seen in the severed spinal cords of adult rats, but scar formation was not seen in the fetal rat cordotomy group. Lack of inflammation and scar formation thus appear advantageous for regeneration of the fetal spinal cord. Between fetal and mature rats, chronological changes in the immunohistochemical reactions of astrocytes and microglia following cordotomy were compared, and the results confirmed many differences. The results of the present study suggest that the presence of activated glial cells around damaged central nervous tissue and the quick disappearance of these cells after injury are important for the repair of damaged central nervous system tissue, and that the role of glial cells in nerve regeneration can change depending on the level of maturity of glial cells or surrounding cells, site of injury, or the state of tissue around the injury.

Animals↗
PubMed2005

Modified Steindler procedure for the treatment of brachial plexus injuries.

A retrospective follow-up study was completed on ten patients who suffered from a brachial plexus injury that was treated with a modified Steindler procedure. The mean postoperative period was 6.8 years. The postoperative elbow joint range of motion was -42 degrees of extension (range -5 degrees to -65 degrees ) and 107 degrees of flexion (range 90 degrees -130 degrees ). Manual muscle testing showed grade 4 or 5 in eight patients and grade 3 in two patients. In the subjective assessment, the patients scored 20 out of 30 points and were able to perform almost all activities with the exception of shoulder elevation. Innervation of the musculocutaneous nerve was evaluated by electromyography and no correlation was seen between preoperative and postoperative amplitude of the biceps brachii by electromyogram. Based on these results, we concluded that a modified Steindler procedure is useful for reconstruction of upper brachial plexus injuries, and recovery of the biceps brachii was difficult to predict by an electromyogram.

Adult↗
PubMed2005

Prefabrication of vascularized bone graft using a combination of fibroblast growth factor-2 and vascular bundle implantation into a novel interconnected porous calcium hydroxyapatite ceramic.

The aim of this study was to create a prefabricated vascularized bone graft using a novel interconnected porous calcium hydroxyapatite ceramic (IP-CHA) by combining vascular bundle implantation and basic fibroblast growth factor (FGF)-2 administration in a rabbit model. Twenty-four Japanese white rabbits were used. The saphenous artery and vein were passed through the hole of the IP-CHA. In an experimental group, 100 microg of FGF-2 was administered into the IP-CHA before implanting the vascular bundle. In the control group, the saline was administered into the IP-CHA before implanting the vascular bundle. Finally, the IP-CHA was placed subcutaneously in the medial thigh. Neovascularization from the vascular bundle was evaluated at 2 weeks after surgery, and osteogenesis was evaluated at 4 weeks. At 2 weeks, the length and density of newly formed vessels were significantly greater in the experimental group than in the control group. Histological evaluation showed osteoid deposition in the pores of the IP-CHA at 4 weeks in the experimental group, whereas no evidence of osteoid deposition was noted in the control group. This study showed the potential of creating a vascularized bone graft of a predetermined size and shape using a combination of FGF-2 and vascular bundle implantation in the IP-CHA.

Animals↗
PubMed2005

Characterization of labeled neural progenitor cells for magnetic targeting.

For many diseases and injuries of the central nervous system, transplantation of neural progenitor cells is being evaluated as a possible treatment option. Although local, intravenous and subarachnoid injections have been reported as administration methods of neural progenitor cells, each of these methods has limitations. More effective and minimally invasive cell delivery systems are necessary for transplanting neural progenitor cells. In this study, we have developed a technique to form magnetically labeled neural progenitor cells for a magnetic targeting system. We demonstrated that neural progenitor cells can couple with magnetic beads, and that the labeled neural progenitor cells preserve the characteristics of non-labeled neural progenitor cells, and that they can be localized by magnetic force in vitro. Labeled neural progenitor cells have the potential to be used in magnetic targeting systems in-vivo models.

Amides↗
PubMed2005

Clinical characterizations and radiologic findings of pure foraminal-type cervical disc herniation: CT discography as a useful adjuvant in its precise diagnosis.

STUDY DESIGN: A retrospective study of 15 cases of pure foraminal type cervical disc herniation (CDH) was performed. OBJECTIVES: The objectives were to describe the clinical characteristics and to assess the radiologic findings. SUMMARY OF BACKGROUND DATA: Although foraminal lumbar disc herniation has been reported, pure foraminal-type CDH has not been fully elucidated. It is not only a rare condition but also a difficult one to diagnose. METHODS: All patients underwent MRI, CT myelography (CTM), and CT discography (CTD). Herniated discs were removed by the posterior approach. Clinical symptoms were evaluated at preoperative and postoperative period. RESULTS: On MRI, 2 cases were positive, 6 cases were suspected, and 7 cases were negative. On CTM, 7 cases were positive and 8 cases were negative; and on CTD, all cases were positive. As to the association between the herniated disc and posterior longitudinal ligament, 12 cases were intraligamentous. The association between the herniated disc and nerve root revealed that 10 discs were at the axillary portion of the nerve root. CONCLUSIONS: Pure foraminal-type CDH may be overlooked with conventional MRI or CTM, so CTD should be performed. It provides valuable and additional information in difficult or ambiguous cases such as pure foraminal type.

Adult↗