Biomedical subjects

N A Margolina

Publications and source records attributed to N A Margolina.

4 recordsLinked to original sources

PubMedDate not supplied

[Permeability of acetylcellulose ultrafiltration membranes with regard to biologically active substances].

The permeability of standard Soviet ultrafiltration membranes prepared from cellulose acetates was investigated with respect to biologically active substances (hemoglobin, trypsin, ribonuclease, vitamin B12, hydroxytetracycline) and inorganic salt (KH2PO4). The arrest of a substance by a membrane of a certain structure depended primarily on the size of the substance macromolecule in the solution. The filtration rate was related to the membrane type, pressure gradient and composition of the filtered solution. Potential use of the tested membranes is described.

Cellulose↗

PubMedDate not supplied

[Preparative isolation of terrilytin components and study of their enzymatic properties].

By ion-exchange chromatography the composition of the fibrinolytic enzyme from Aspergillus terricola-terrilytine-was studied. The preparation contained five components that differed in their substrate specificity. Three of the components and proteolytic and fibrinolytic activities. The isolated fractions were examined with respect to their pH stability, pH optima (casein, hemoglobin and fibrin used as substrates), fibrinolytic and fibrinohenolytic activities.

Aspergillus↗

PubMedDate not supplied

[Study of ultrafiltration of trypsin solutions].

The dependence of the rate of trypsin ultrafiltration on the concentration, pressure and structure of membranes was studied. During ultrafiltration of diluted trypsin (0.3 mg/ml), water and sodium chloride the flow rate increased linearly with a pressure increase in the range of 0.4-4.2 kg/cm2. During ultrafiltration of trypsin solutions of a concentration of 1 mg/ml and over at a pressure of 2-3 kg/cm2 deviations from linear proportionality occurred which enhanced with an increase in the protein concentration and a decrease in membrane permeability.

Binding Sites↗

PubMedDate not supplied

[Trypsin concentration by ultrafiltration].

Criteria for the selection of membranes and optimal conditions for the trypsin concentration by ultrafiltration through acetylcellulose membranes have been developed. The possibility of a repeated concentration of trypsin by means of these membrances has been shown.

Trypsin↗