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N Atanasov

Publications and source records attributed to N Atanasov.

At least 37 records · Page 2Linked to original sources

Electrophoresis of water-insoluble leptospiral proteins and the taxonomy of Leptospira.

The cellular debris obtained after lysis of Leptospira were solubilized by sodium dodecyl sulfate and studied by polyacrylamide electrophoresis, comparing the molecular weight and relative concentration of the protein bands. The percentage similarity of 30 characteristic bands from 21 Leptospira strains, belonging to the main pathogenic and some saprophytic serogroups, was calculated; A partial matching of the derived dendrogram with the other classifications was observed.

Bacterial Proteins↗

[Disc electrophoresis of urinary proteins. Changes after exertion].

The authors examined, by means of disc electrophoresis, urine, obtained from football players before and after a match of the championship of the "A" republican football group. Proteinuria after effort was manifested by a considerable excretion of albumin, but there were also serum proteins: postalbumin, transferin, gamma-globulins and fractions found at the place of alpha2-macroglobulins till a complete resemblence of serum electrophoresis. Electrophoresis of lactate-dehydrogenase revealed the presence only of two quick fractions, mainly LDH-1, which increased after effort as well. The fractions of nonspecific esterases were of predominantly tissue origin but after effort of serum origin. The results were discussed in connection with various mechanisms, which could cause proteinuria after physical effort.

Albumins↗

[Proteinases in the muscles of embryos and hens and and in transplantable muscle sarcoma].

The authors examined the activity of acid proteinases in extracts, obtained by 0,05 M KCl from thigh muscles of 12-and 18-day hen embryos and of a 3-year hen and from muscle sarcoma 12--14 days after its transplantation on thigh muscles of a hen. The activity of the fractions obtained from extracts by gel chromatography on sefadex G-200, was also determined. The highiest activity per unit of protein was established in extracts of embryonal muscles, but the lowest -in cultures of hens. Sarcoma took medean position. Seven fraction of acid proteinases were found in the muscles and in the sarcoma. In most cases sarcoma took mean position among embryonal muscles and muscles of hens in percentage ratios of these fractions. The highiest mollecular (the earliest flowing) acid proteinase fractions in the extract of hen muscles had insignificant activity, but in extracts of sarcoma they had almost 20% of the total activity.

Animals↗