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Biomedical subjects

N Bhamarapravati

Publications and source records attributed to N Bhamarapravati.

At least 91 records · Page 5Linked to original sources
PubMed1977

Replication of dengue-2 virus in Aedes albopictus mosquitoes. An electron microscopic study.

Sequential electronmicroscopic studies of Aedes albopictus mosquitoes infected with dengue-2 virus showed replication of virus particles and vesicular structures confined to cells having an active, rough, endoplasmic reticulum. Small granules not found on the surface of virions produced in mammalian cells were seen on the envelopes of the virus particles. There was no evidence of nuclear involvement as described in infected mouse neurones, and there was no apparent impairment of cellular function. Substantial viral replication was confined to cells of the salivary glands and nervous tissue, with lesser involvement of midgut, hemocytes, epidermal cells, fatbody, and foregut.

Aedes↗
PubMed1976

Serum-soluble malarial antigens and immune complex nephritis in Plasmodium berghei berghei infected mice.

Swiss albino mice infected with Plasmodium berghei berghi showed the serum-soluble malarial antigen and antibody on day 10 of infection onward. Immune complex nephritis in these mice developed on the seventh day after inoculation. The infected kidneys revealed the deposition of mouse gamma globulin, mouse beta1C globulin and malaria antigen along the capillary wall of the glomeruli. Proteinuria was detected on seventh day of infection. Serum-soluble malaria antigen in probably responsible for forming the soluble immune complex which causes glomerulonephritis in infected mice.

Animals↗
PubMed1976

Immunofluorescent staining of the surfaces of lymphocytes in suspension from patients with dengue hemorrhagic fever.

Immunofluorescent staining of lymphocytes suspensions from 55 of 62 patients with dengue hemorrhagic fever was positive for dengue antigen and human beta1C/a-globulin on the surface, from the second ay before shock or subsidence of fever. The percentages of positive staining of both components gradually increased to a maximum on the day of shock or subsidence of fever. B lymphocytes increased during the course of the disease. Neither dengue antigen nor human beta1C/a-globulin was detected on the surface of the lymphocytes from normal controls or patients with other diseases. By double immunofluorescent staining with different colors of fluorochromes, antidengue antibody with fluorescein isothiocyanate and antihuman gamma -globulin or antihuman beta1C/a-globulin with lissamine rhodamine B on the same lymphocytes revealed dengue antigen appearing only on B lymphocytes. The human beta1C/a-globulin and dengue antigen were located on the surface of the same lymphocytes. The pattern of the staining by both components showed fine and coarse irregular granules over the lymphocyte surface. The fluorescent granules seemed to be on the surface but not in the intracellular vacuoles of the lymphocytes.

Alpha-Globulins↗
PubMed1976

Glomerular changes in dengue hemorrhagic fever.

Percutaneous needle biopsies were performed on the kidneys of 20 patients who had dengue hemorrhagic fever with some clinical manifestation of renal impairment. In ten cases IgG or IgM, or both, and C3 could be localized in the glomeruli, using the fluorescent antibody technique. In those cases in which immune complex was found, the biopsy had been done in the second week after the onset of fever. Electron microscopy showed focal thickening of the glomerular basement membrane, with hypertrophy of mesangial cells at the sites where the immune complex was shown. Dense, spherical particles, 40 to 50 nm in diameter, were found in 12 cases. Those particles were carried into the glomeruli by monocyte-like cells.

Adolescent↗
PubMed1974

Filamentous structures in dengue type 3 virus infected mouse neurones.

Dengue type 3 (H-87) virus was inoculated into suckling mouse brain and animals were sacrificed at 24 hr intervals. Parallel filamentous structures were found in infected neurones in close association with virus particles in the distended endoplasmic cisternae. They were usually arranged in a crystalloid pattern, oriented in different directions within the cisternae. Faint helical features were sometimes observed. These filamentous structures measured 15-25 nm in width and varied in length. Their possible involvement with viral material or a viral core is postulated.

Animals↗
PubMed1973

Dengue virus infection of mice: morphology and morphogenesis of dengue type-2 virus in suckling mouse neurones.

In dengue type-2 virus-infected neurones of suckling mice, formation of single-membrane vesicles is observed in the distended cisternae of the endoplasmic reticulum mostly of the perinuclear zone around 72 h after inoculation. Electron-dense 50-nm virus particles are arranged in chains in these distended cisternae; some form small crystalloid aggregates. Aberrant particles of different shapes are also seen in the distended cisternae about the same time that the virus particles appear. Parallel filamentous structures are occasionally observed in the cisternae that contain very few virions, either characteristic or aberrant. Increasing cytopathic changes are present after 75 to 96 h. There is an intense vesicular formation. Large numbers of virions and aberrant particles are seen either in the endoplasmic reticulum cisternae or smooth membrane vesicles. They are spread throughout the neurocytoplasm, extending into the dendrites. Dengue virions which are enclosed in fairly intact membrane-bound vesicles are released during cytolysis of the neurones. Morphogenesis of dengue virus type 2 is discussed.

Animals↗