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N C Jain

Publications and source records attributed to N C Jain.

At least 55 records · Page 3Linked to original sources
PubMed1987

In vitro platelet release by rat megakaryocytes: effect of metabolic inhibitors and cytoskeletal disrupting agents.

Development of an in vitro visual assay facilitated the study of large numbers of megakaryocytes undergoing proplatelet formation in short-term cultures. Approximately 9% of megakaryocytes formed platelets during a 24-hour period. In the presence of an inhibitor of anaerobic glycolysis (NaF), proplatelet formation was inhibited, whereas inhibitors of respiration (NaCN) did not significantly (P greater than 0.05) decrease proplatelet formation. Presence of the microtubule-disrupting agents colchicine and vincristine sulfate in culture medium inhibited proplatelet formation, whereas the microfilament-disrupting agent cytochalasin B had a less pronounced inhibition.

Animals↗
PubMed1987

In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.

A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.

Animals↗
PubMed1986

Platelet dysplasia associated with megakaryoblastic leukemia in a dog.

In a case of megakaryoblastic leukemia in a dog, definitive diagnosis was made through use of specific cytochemical stains of circulating and bone marrow blast cells. Morphologic and functional abnormalities of platelets were revealed through transmission electron microscopy and in vitro aggregation of platelets after addition of adenosine diphosphate.

Animals↗
PubMed1986

Scanning electron microscopic studies of megakaryocytes and platelet formation in the dog and rat.

Megakaryocyte morphology and platelet formation in canine and murine bone marrows were studied by scanning electron microscopy. In situ-fixed bone marrow preparations and cell suspensions of bone marrow provided complementary information for the 2 species (dogs and rats). Cylindrical processes (proplatelets) of variable length and thickness, originating from the megakaryocyte surface, were in the larger marrow sinusoids and the central vein. Regional constrictions along the length of proplatelets, particularly near their apical region, and the presence of fragments of such processes supported the concept of platelet formation through segmentation of proplatelets. Megakaryocytes presented varied morphology. Surface features resembling platelets were observed on megakaryocytes, indicating that platelets may have been released through surface budding. In conclusion, megakaryocytes formed long proplatelet processes that actively migrated to venous sinusoids to release platelets by fragmentation. Scanning electron microscopy analysis revealed a complex and variable megakaryocyte surface topography. The platelet-like structures on megakaryocyte surfaces may represent platelet release by a budding mechanism. The similarity between murine platelet release and canine platelet release demonstrates that data from rodent models may be applicable to nonrodents.

Animals↗
PubMed1983

Antibacterial activity of bovine blood neutrophils and their cationic proteins.

Six cationic protein bands were identified in extracts of both normal bovine blood neutrophils and their granules subjected to polyacrylamide disc gel electrophoresis. Band 1, the most cationic protein, was as positively charged as crystalline egg white lysozyme, and band 2 was as positively charged as ribonuclease. Antibacterial activity of various leukocyte fractions and cationic proteins was determined in vitro for Enterobacter aerogenes strain 2412-2. Antibacterial activity of various preparations was proportional to amount of test material present and varied inversely with number of bacteria in the test system. Comparative evaluations revealed that the neutrophil granule fraction had greater antibacterial activity than the whole cell homogenate or individual cationic proteins. Among cationic proteins, band 1 had the highest antibacterial activity, band 2 had the lowest, and band 3 had intermediate antibacterial activity. Cationic protein bands 4, 5, and 6 exhibited growth stimulatory rather than a growth inhibitory activity.

Animals↗
PubMed1983

Using red blood cell creatine concentration to evaluate the equine erythropoietic response.

Red blood cell creatine concentration was examined to determine its association with the equine erythropoietic response. Studies were conducted on 9 healthy horses, 4 healthy ponies, 24 anemia horses, and 2 horses in which anemia was experimentally induced. A modified Jaffe reaction was used to measure RBC creatine concentration. The mean RBC creatine concentration of the 9 healthy horses was 5.72 +/- 0.42 mg/dl, and that of the 4 healthy ponies was 2.59 +/- 0.31 mg/dl. Density-separation of erythrocytes from the healthy horses revealed significantly higher (P less than 0.001) creatine content (7.72 +/- 0.57 mg/dl) in the young RBC populations than in the old RBC populations (4.03 +/- 0.27 mg/dl). The RBC creatine content was assayed in 19 hot-blooded horses which were anemic due to a variety of causes. Of these anemic horses, 12 with PCV between 25% and 30% had a mean RBC creatine concentration of 6.12 +/- 0.46 mg/dl. The 7 other anemic horses with PCV less than 25% had a mean RBC creatine value of 6.07 +/- 0.12 mg/dl. Bone marrow films were examined from 5 anemic horses and in the 2 horses in which anemia was experimentally induced. The RBC creatine concentration correlated positively (P less than 0.001) with the reticulocyte count in the bone marrow and negatively with the myeloid-erythroid ratio (P less than 0.001).

Anemia↗
PubMed1982

Haematological studies on normal lactating Indian water buffaloes.

Haematological studies were conducted on 50 clinically normal lactating Murrah buffaloes in India. The range and mean (with one standard deviation), respectively, for the parameters examined were: red blood cells, 5.07 to 8.27, 6.54 +/- 0.77 million per microliter; haemoglobin, 9 to 13.5, 11.1 +/- 0.96 g/dl; packed cell volume, 0.26 to 0.34, 0.31 +/- 0.02; mean corpuscular volume, 40.6 to 55.2, 48.2 +/- 4.6 fl; mean corpuscular haemoglobin concentration, 30.9 to 38.5, 35.2 +/- 2.34 g/dl; mean corpuscular haemoglobin, 13.5 to 20.5, 17.10 +/- 1.85 pg; icterus index, 2 to 5, 2 +/- 1.25 units; erythrocyte sedimentation rate, 17 to 69, 53 +/- 12.3 mm at one hour; plasma protein, 6 to 9, 7.8 +/- 0.7 g/dl; fibrinogen, 0.2 to 0.8, 0.37 +/- 0.2 g/dl; reticulocytes, 0 per cent; white blood cells, 6250 to 13,050, 9676 +/- 1789 microliters; band cells, 0 to 1, 0.2 +/- 0.34 or 0 to 106, 18 +/- 40/microliters; neutrophils, 13 to 54, 32.9 +/- 8.74 per cent or 1285 to 6893, 3257 +/- 1262/microliters; lymphocytes, 26 to 75, 52.7 +/- 12.0 per cent or 2554 to 9637, 5065 +/- 1595/microliters; monocytes 1 to 11.5, 5.9 +/- 2.63 per cent or 63 to 1349, 584 +/- 301/microliters; eosinophils, 2 to 14.0, 6.9 +/- 4.64 per cent or 170 to 1471, 592 +/- 452/microliters and basophils, 0 to .5, 1.4 +/- 1.02 per cent or 0 to 326, 131 +/- 98 microliters. Normal species characteristics evident from this investigation included average size of the erythrocytes similar to that in cattle, low icterus index, conspicuous erythrocyte sedimentation rate, absence of reticulocytes and predominance of lymphocytes over neutrophils.

Animals↗
PubMed1982

Dermatomyositis.

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Adolescent↗