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Biomedical subjects

N D Fanchenko

Publications and source records attributed to N D Fanchenko.

At least 91 records · Page 5Linked to original sources
PubMed1975

[The biological role of estriol].

It was demonstrated on the uteri of women and guinea pigs that estriol (in vitro) possessed a marked affinity to the estradiol-binding system of human and guinea pig uteri; the activity of steroid-receptor interaction of estriol in vitro constituted 9.4% for guinea pigs and 17% for man in relation to the estradiol activity. Administration of estriol to guinea pigs in vivo in a dose of 0.25-0.5 mg led to a sharp reduction of the estradiol-binding capacity of the receptor system of the uterus. It is supposed that there existed a competitive relationship between estradiol and estriol for binding with the active centres of the receptor proteins of the uterus.

Animals↗
PubMedDate not supplied

[Comparative determination of progesterone in the blood plasma by radioimmunological and chromato-mass-fragmentographic methods].

A comparative determination of progesterone in the blood plasma was performed using a radioimmunoassay (RIA) and 2 variants of a chromato-mass-fragmentographic (CMF) method. A deuterated internal standard, capillary gas-liquid chromatography and 3-enol-3-tret-BDMS and 3.20-bis-enol-bis-BDMS progesterone ethers as chromatographied derivatives were employed. Both derivatives made it possible to perform CMF determination using simplified variants of the method. The variants of the CMF method were employed as references for RIA of progesterone. Comparison of the results of the CMF and RIA methods indicated RIA significance (r = +0.99) in using antiserum to the conjugate 3-(O-carboxy methyl)-oxime of progesterone with bovine serum albumin injected to rabbits.

Evaluation Studies as Topic↗
PubMedDate not supplied

[Intracellular distribution of estrogen receptors in the human endometrium].

Fifty samples of endometrial tissue from healthy women with regular menstrual cycle and from patients with uterine myoma, ovarian cystoma, uterine hypoplasia and endometriosis were studied. The amount of cytoplasmic (total and free) and nuclear estradiol-binding sites as well as estradiol (E2) concentration in the endometrial cells were estimated. Level of E2 in the peripheral blood plasma did not correlate with its content in endometrial cells. At the same time, amount of cytoplasmic estrogen receptors bound with E2 did not correlate with the estradiol content in cytoplasm. The data obtained suggest the leading role of the cytoplasmic estrogen-receptors interaction in development of hormonal effect on the tissue. The formation of hormone-receptor complexes appears to be controlled by some additional factors of unknown nature.

Cell Nucleus↗
PubMedDate not supplied

[Comparative determination of norethisterone in the blood plasma by radioimmunological and chromato-mass fragmentographic methods].

Radioimmunoassay (RIA) and two variants of the chromato-mass fragmentographic (CMF) method were used for comparative determination of norethisterone (NET) in the blood plasma. A deuterated internal standard, capillary gas-liquid chromatography and chromatographed derivates: 3-enol-3-tret-butyldimethylsilyl-17-tri-methysilyl and 3-enol-3,17-bis-tret-butyldimethylsilyl NET esters were used. Both derivates provide for CMF determination after simplified variants. They were used as references for RIA determination of NET. Comparison of the CMF and RIA results indicate to the significance of RIA determination (r = 0.979) while employing antiserum to NET conjugate 3-(0-carboxymethyl)-oxime with bovine serum albumin injected to rabbits.

Evaluation Studies as Topic↗