Biomedical subjects

N I Shalbueva

Publications and source records attributed to N I Shalbueva.

7 recordsLinked to original sources

PubMed2003

[Distribution of the labeled lipoproteins in the myocardium during perfusion of the isolated rat heart and adrenaline administration].

The distribution of different classes of colloidal gold-labeled lipoproteins (LP) was studied at the ultrastructural level in rat myocardium using the isolated heart perfusion following the adrenaline stimulation. The distribution of label was examined in endotheliocytes, macrophages and in the interstitial space. The addition of adrenaline to Krebs solution during 30 min of heart perfusion in vitro was shown to increase the amount, but to decrease the velocity of penetration of labeled LP of very low and low density into the myocardium. The intensive utilization of lipid component from LP in the conditions of stimulation provides for the enhanced metabolic requirements of the cardiomyocytes and good preservation of their ultrastructure. It is possible that colloidal gold-associated protein component of LP is incorporated by the endothelium more slowly. The label penetrates into the interstitium and is accumulated in the activated macrophages. If high density LP are added together with adrenaline into the perfusion medium the label does not leave the capillary wall, while macrophages and cardiomyocytes acquire the ultrastructural features of high functional activity.

Animals↗

PubMed2002

[Penetration of lipoproteins through a capillary wall and their effects on the cardiomyocyte ultrastructure during in vitro heart perfusion].

The colloidal gold-labeled lipoprotein (LP) distribution in the myocardium after the 30 min perfusion of isolated working rat heart was studied by electron microscopy. LP of physiological concentrations are shown to be able to interact with the capillary wall and they can be incorporated by endotheliocytes. The velocity and transportation mechanisms of different classes of LP differ. Very low density LP are the most intensively taken up, low density LP enter into interstitium more rapidly than others, high density LP do not leave the capillary wall at all. The passages of labeled LP through endotheliocytes by the receptor-mediated as well as the non-receptor manners are revealed. The transportation through the widened intercellular junctions may be supposed for low density LP. For the fist time LP addendum into the perfusion medium was shown to provoke the activation of interstitial macrophages. During the perfusion duration they take inside and accumulate labeled very low density LP and low density LP in their lysosomes. The internalization may be performed by the specific endocytosis or by the simple phagocytosis. The qualitative and morphometrical analyses show that LP preserve the capillary and cardiomyocyte ultrastructure from perfusion injuries. One may suppose that there are interrelations between the capillary endothelium, the interstitial macrophages and the parenchymal cells in myocardium for realization of plasma LP effects.

Animals↗

PubMed2001

Dynamics of structural and functional changes in hepatocyte mitochondria of senescence-accelerated OXYS rats.

Electron microscopy showed signs of degenerative dystrophic changes in hepatocytes and structural decompensation of mitochondria in senescence-accelerated OXYS rats in comparison with Wistar rats. These signs were detected in the presence of liver mitochondria dysfunction in OXYS rats: reduced oxygen consumption rate in all metabolic states, respiratory control volume, ADP/O ratio. transmembrane potential, and phosphorylation rate. The revealed disorders in mitochondrial structure and function are the key factors in the pathogenesis of accelerated aging in OXYS rats and visceropathies characteristic of these animals.

Adenosine Triphosphate↗

PubMed2001

Involvement of adenine nucleotide carrier in the mechanism of the apolipoprotein C induced decrease of membrane potential in rat liver mitochondria.

Apolipoprotein C (apo C) was shown to decrease the Ca2+ capacity and membrane potential of mitochondria isolated from rat liver. The specific ligands of adenine nucleotide carrier, ADP and carboxyatractyloside (CAT), inhibited the effect of apo C on the mitochondrial membrane potential. The effect of ADP and CAT was revealed in the absence of Ca2+. We conclude that in the presence of apo C, adenine nucleosides carrier transforms into a pore, and this causes the decrease in the membrane potential of the mitochondria. ADP and CAT support the primary conformation of the carrier and therefore inhibit the effect of apolipoprotein C.

Adenosine Diphosphate↗

PubMed2000

Effects of apolipoproteins C on oxidative phosphorylation in rat liver mitochondria.

It is shown that apoC-III, but not other apoC proteins, components of very low density lipoproteins (apoC-I, apoC-II, apoC-III), reduced the rate of mitochondrial respiration in various metabolic states. This effect depended on the dose of apoprotein, type of oxidized substrate, and the presence of Ca ions in the incubation medium. ApoC-III completely blocked oxidative phosphorylation during oxidation of palmitoyl carnitine by mitochondria, while the respiration rate in metabolic state 4 remained unchanged.

Animals↗

PubMedDate not supplied

[Use of mirtilene forte and adrusen zinco for correction of oxidative lesions in mitochondria in rats OXYS with inherited hyperproduction of free radicals].

A long-term uptake of a diet supplemented with Mirtilene Forte (Vaccinum myrtillus extract) or Adrusen Zinco (a vitamin E complex with zinc, copper, selenium and omega-3 polyunsturated fatty acid) improved the functional state of liver mitochondria in OXYS rat strain. Adrusen Zinco sharply decreases the level of protein and lipid oxidation products. Possible mechanisms of the drug action are considered in relationship with their antioxidant properties.

Animals↗

PubMedDate not supplied

[The activity of trypsin and proteolytic inhibitors in complicated forms of appendicitis in children].

The activity of trypsin, alpha 1-proteinase inhibitor, and alpha 2-macroglobulin (MG) was measured in the blood plasma of patients with destructive appendicitis and localized and generalized peritonitis during admission to hospital and for 3 weeks after surgery. A deficit of MG was found in all forms of these diseases. A decreased activity of MG in the blood plasma is apparently a risk factor for emergency inflammatory diseases of abdominal organs. After appendectomy a more demonstrative index of patient's status is trypsin activity, an increase of which is most of all expressed in generalized peritonitis. Quantitative assessment of the trypsin-inhibitor system activity before and after surgery is necessary for a more accurate diagnosis and for monitoring the quality of treatment.

Adolescent↗