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Biomedical subjects

N J Moore

Publications and source records attributed to N J Moore.

4 recordsLinked to original sources

Human appropriation of photosynthesis products.

Previous global estimates of the human impact on terrestrial photosynthesis products depended heavily on extrapolation from plot-scale measurements. Here, we estimated this impact with the use of recent data, many of which were collected at global and continental scales. Monte Carlo techniques that incorporate known and estimated error in our parameters provided estimates of uncertainty. We estimate that humans appropriate 10 to 55% of terrestrial photosynthesis products. This broad range reflects uncertainty in key parameters and makes it difficult to ascertain whether we are approaching crisis levels in our use of the planet's resources. Improved estimates will require high-resolution global measures within agricultural lands and tropical forests.

Agriculture↗

Efficacy of Nucleic Acid Probes for Detection of Poliovirus in Water Disinfected by Chlorine, Chlorine Dioxide, Ozone, and UV Radiation.

MilliQ water was inoculated with poliovirus type 1 strain LSc-1 and was treated with disinfectants, including chlorine, chlorine dioxide, ozone, and UV light. No relationship between probes and plaque assays were seen, demonstrating that viral nucleic acids were not destroyed. These findings suggest that nucleic acid probes cannot distinguish between infectious and noninfectious viruses and cannot be used in the evaluation of treated waters.

Journal Article↗

Evaluation of radioactive and nonradioactive gene probes and cell culture for detection of poliovirus in water samples.

Five nonradioactive probe assays were evaluated by using chemiluminescent and colormetric signals, along with two isotopic assays and cell culture, for the detection of poliovirus in concentrated water samples. In environmental samples, a 100% correlation existed between digoxigenin and single-stranded [32P]RNA probes. All probe assays detected more positive samples than the cell culture did.

Animals↗

Type-specific human papillomavirus detection in formalin-fixed, paraffin-embedded tissue sections using nonradioactive deoxyribonucleic acid probes.

DNA probes directly conjugated to horseradish peroxidase have been used successfully to detect human papillomavirus (HPV) types 6/11, 16, and 18 in formalin-fixed, paraffin-embedded tissue sections. By using silver enhancement of a heavy metal-modified diaminobenzidine precipitate, the sensitivity of human papillomavirus detection was significantly increased without compromising specificity. In studies comparing the specificity of the horseradish peroxidase-labeled probe/silver enhancement system to that of a biotinylated-DNA probe/streptavidin-alkaline phosphatase system, the former was found to be superior.

3,3'-Diaminobenzidine↗