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PubMed · 2172649

Type-specific human papillomavirus detection in formalin-fixed, paraffin-embedded tissue sections using nonradioactive deoxyribonucleic acid probes.

T W Higgs·N J Moore·D Y Badawi·F E Taub
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Abstract

DNA probes directly conjugated to horseradish peroxidase have been used successfully to detect human papillomavirus (HPV) types 6/11, 16, and 18 in formalin-fixed, paraffin-embedded tissue sections. By using silver enhancement of a heavy metal-modified diaminobenzidine precipitate, the sensitivity of human papillomavirus detection was significantly increased without compromising specificity. In studies comparing the specificity of the horseradish peroxidase-labeled probe/silver enhancement system to that of a biotinylated-DNA probe/streptavidin-alkaline phosphatase system, the former was found to be superior.

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3,3'-DiaminobenzidineDNA Probes, HPVHistological TechniquesHorseradish PeroxidaseHumansPapillomaviridaeJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T W Higgs, N J Moore, D Y Badawi, F E Taub. 1990. Type-specific human papillomavirus detection in formalin-fixed, paraffin-embedded tissue sections using nonradioactive deoxyribonucleic acid probes.. https://pubmed.ncbi.nlm.nih.gov/2172649/

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