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Biomedical subjects

N Manolescu

Publications and source records attributed to N Manolescu.

At least 19 recordsLinked to original sources
PubMed1983

Changes concerning the external structure of peritoneal macrophages, due to the DQ-12 standard dust.

The cytotoxic activity of the DQ-12 standard dust on the external structure of peritoneal macrophages, after intraperitoneal introduction, was investigated, vs nontreated macrophages, and kept in in vitro cultures for 3, 6, and 24 hr. The alterations related to the external structure were investigated by scanning electron microscopy (SEM), clearly showing that cell damage can take place when the dust contacts the external surface of the cell, as already shown by the work of Johnson and Davies (paper presented at the International Workshop on the in Vitro Effects of Mineral Dusts, South Wales, U.K., 1979) and Miller (Environ. Res. 20, 162-182, 1979).

Animals↗
PubMed1983

Relationship between the lead concentration in hair and occupational exposure.

The lead content of hair in workers occupationally exposed was correlated with the blood lead concentration. Determinations of lead in blood and hair were performed by electrothermal atomic absorption spectrophotometry in two exposed groups and a control group. A significant correlation was observed between the blood lead and hair lead concentrations, and a regression analysis showed an exponential accumulation of the lead content in hair, simultaneously with the increase of the values in blood. The colour of the hair and the age of the subject did not influence the lead accumulation in hair in the occupationally exposed subjects. The assessment of lead in hair is considered a useful screening test in estimating occupational exposure.

Adult↗
PubMed1981

[Isolation of a Pasteurella multocida bacteriophage].

Lysogenesis testing of 84 Pasteurella multocida strains resulted in detection of one lysogenic strain from which phage B225 was isolated. B225 lysed only one Pasteurella multocida strain isolated from cattle. The Pasteurella phage, when examined by electron microscopy, showed a hexagonal head and a non-contractile, Flexible tail, It could be attached, consequently, to Group B according to Bradley (1967).

Animals↗
PubMedDate not supplied

[Electron microscopic observations on the existence of "deflagellation tolerance" processes capable of explaining the selective isolation of vibrios in semisolid agar].

The authors identified a group of substances (flagellar depression factors--FDF) which suppress the mobility of enterobacteria (immobilization) but do not influence the Vibrio movements (tolerance). FDF do not affect the vital processes, multiplication and formation of colonies. Electron microscopic interpretation showed that, in the usual media, enterobacteria have characteristic peritrichous cilia, whereas in media with FDF they are denuded of their flagellation formation. As such a process of flagellation depletion seems to be not known, we suggest the term "deflagellation" to name it. In a completely different way, the vibrios keep their single cilium located apically and implicitly their moving ability. These behaviour differences are evident in semisolid agar with FDF. The deflagellated enterobacteria are, therefore, immobilized, and multiply, forming small punctual colonies, of 0.5-1 mm phi, whereas the vibrios tolerating FDF develop and at the same time move in the liquid agar, mass, forming large colonies, of 8-14 mm phi. The electron microscopic observations presented show the existence of a "deflagellation tolerance" process and also explain the specific typology of the colonies of vibrios and enterobacteria formed in liquid agar with FDF.

Agar↗
PubMedDate not supplied

HBsAg positive secondary autoimmune active chronic hepatitis. Immunogenetic aspects.

Observations were made in a group of 120 patients with active chronic hepatitis (ACH), using demonstration of HBsAg presence, structural study of the peripheral blood mononuclear cell population by means of scanning electron microscopy (SEM) and typing of HLA-A, B and C antigens. From the whole group 22 patients were proved to have an autoimmune form of ACH secondary to infection with hepatitis B virus (HBV). The authors consider that the diagnostic value of the ratio between B and T lymphocytes (studied by SEM) is relative but the demonstration of an increased percentage of monocytomacrophage cells and the presence of "killing phenomenon" clearly reflect a more severe hepatic inflammation. A higher incidence of the HLA-Bw35-Cw4 was observed particularly in the HBsAg positive forms of ACH and in the HBsAg positive secondary autoimmune forms. The authors discuss the utility of these observations when the opportunity of corticotherapy has to be established.

Adult↗
PubMedDate not supplied

Identification and study of the various leukocytes, particularly of the leukemic ones. (Principles and some technical details).

Based on the main data from the specialty literature and on their own experience in the field, the authors present the principles and some details of the classical and the modern techniques used to identify and study the various leukocytes and especially the leukemic ones. These different methods of investigation are included into the wide categories of histologic, cytomorphologic, cytochemical, cytoenzymatic, cytoimmunological, cytogenetic, cell culture and other complementary techniques. The great progress achieved in this domain was favoured by the development of light, contrast phase and fluorescent microscopy and of the new techniques of transmission and scanning electron microscopy--whose utility for the study of the white blood cells, and particularly of the leukemic ones, is demonstrated in this paper by numerous suggestive illustrations.

Bone Marrow Cells↗
PubMedDate not supplied

Examination of human and animal leukemic cells by high contrast scanning electron microscopy.

Investigation of human or animal (sheep and ox) leukemic cells by scanning electron microscopy enabled the identification and study of some ultrastructural details of the cell surface. A more detailed visualization by the high contrast technique of the same cell surface was obtained, in this work, for the first time in the biological field. This technique allows a better interpretation of any zone of the preparation examined, irrespective of the species from which the cells are collected.

Animals↗
PubMedDate not supplied

Propagation of equine infectious anemia virus in horse cell cultures.

The Wyoming strain of equine infectious anemia virus was adapted to cell cultures by 7 passages in horse leukocytes and 14 passages in fetal equine dermal and kidney cells. The virus was made evident by electron microscopy and immunodiffusion tests with antigens prepared from culture fluids.

Animals↗