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Biomedical subjects

N Manolescu

Publications and source records attributed to N Manolescu.

25 records · Page 2Linked to original sources
PubMedDate not supplied

Animal DNA viruses analyzed by colour electron microscopy.

Black-and-white and colour transmission electron micrographs of three DNA viruses (infectious bovine rhinotracheitis virus, bovine adenovirus type 5 and Molluscum contagiosum) are comparatively presented. The colour images have been obtained by an original method of computer-assisted analogic processing of black-and-white micrographs, using the Romanian electronic device OPTIMISCOP. A comparative analysis of the two types of images demonstrates the variety of additional information provided by the new processing technique.

Adenoviridae↗
PubMedDate not supplied

Aspects of the T and B lymphocyte surface architecture in chronic internal diseases with immune component. Scanning electron microscopy studies.

Investigations on the T and B lymphocyte surface architecture were carried out by means of scanning electron microscopy (SEM) in 102 patients with various chronic internal diseases such as chronic hepatitis, diabetes mellitus, systemic lupus erythematosus, chronic lymphocytic leukemia, a.o. The clinical and biological examination performed in these cases reveled also some cytochemical, biochemical and immune disturbances. Emphasis is laid on the superiority of SEM, as compared with the E and EAC rosette tests, for the identification of T and B lymphocytes. Moreover, by detecting certain cellular anomalies, SEM can provide useful indications regarding the diagnosis, prognosis and therapy of the above mentioned diseases. Association of SEM with other clinical and laboratory investigations can either confirm, disapprove or even enhance the value of the morphological findings by electron microscopy.

B-Lymphocytes↗
PubMedDate not supplied

Some ultrastructural aspect of T and B lymphocytes and their significance in chronic internal diseases with immune component.

Some peculiar aspects of T and B lymphocytes from patients with chronic internal diseases with immune component such as: chronic hepatitis (CH), rheumatoid arthritis (RA), diabetes mellitus (DM), systemic lupus erythematosus (SLE), chronic lymphocytic leukemia (CLL) and others, could be detected using transmission electron microscopy (TEM) or scanning electron microscopy (SEM) in association with cytomorphologic, cytoenzymochemical and/or cytoimmunologic methods. Among the more frequent aspects observed were the change of the T and B lymphocyte balance and the presence of giant nucleoli associated with RNA hyposynthesis. This latter aspect was proved to be a sign of unfavourable prognosis and of resistance to immunosuppressive treatments in diseases such as: RA, SLE and CLL.

B-Lymphocytes↗
PubMedDate not supplied

Changes of human platelets studied by scanning electron microscopy in acute myocardial infarction.

The formation of platelet aggregates has been suggested to be the initial step in coronary occlusion and the subsequent myocardial infarction (AMI). By scanning electron microscopy we followed: the platelet morphology and aggregation, the macrophage (M) morphology and the lymphocyte (T, B) count and structure in 11 patients with AMI and in 18 patients with unstable angina (UA). Generally, in the patients with AMI, most of the platelets presented relatively frequent (42.5%) or very frequent pseudopodia (35.27%) and the network of surface extensions was associated with several huge platelet aggregates in 6 patients. The mean lymphocyte count was: T = 40.36 +/- 23.95%; B = 28.09 +/- 7.38%; M = 31.54 +/- 21.25%. In the patients with unstable angina the proportion of platelets with pseudopodia was more reduced, namely, that of platelets with relatively frequent pseudopodia was 33.8% and with very frequent pseudopodia was 27.8%. The mean lymphocyte count was: T = 40.30 +/- 20.24%; B = 34.6 +/- 14.39%; M = 25 +/- 10.50%. These data indicate that platelet changes and the formation of aggregates can be an important factor in the occurrence of AMI. The change of immunocompetent cell count in both groups of coronary patients suggests the association of an immunologic process in coronary atherosclerosis.

Adult↗
PubMedDate not supplied

"Killer"-type phenomena in the immune pathology of some chronic internal diseases. (Scanning electron microscopy studies).

Aspects of cytotoxicity followed by cytolysis have been described by many authors in neoplasias. Using scanning electron microscopy, the authors have investigated the cellular population from 179 patients with various chronic internal diseases such as: chronic hepatitis, diabetes mellitus, systemic lupus erythematosus, scleroderma, chronic lymphocytic leukemia. In some of the forms of disease with severe clinical and biological phenomena, they have detected the presence of "killer" type phenomena, having as target cells either the monocytomacrophage or the T or B lymphocytes. Iconographic aspects are demonstrating the presence of this phenomenon, observed particularly in the peripheral blood. Detection of such phenomena is assumed to provide information on prognosis of the chronic disease investigated and on the effects of therapy at cellular level.

Cytotoxicity, Immunologic↗
PubMedDate not supplied

Electron optic investigations on influenza virus A(H1N1) interacted with some aromatic compounds.

The morphological changes caused by the interaction of influenza virus A(H1N1) with three fluorescent aromatic compounds (a vinyl-sulfonic and an isocyano naphthalic acid derivative and the 4-acetylamino-naphthalene-1,8-dicarboxylic acid) were studied in the electron microscope. The intensity and type of the alterations depend on the chemical structure of the compounds and can be correlated with their effect on the biological activities of influenza virus.

Fluorescent Dyes↗