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N Morimoto

Publications and source records attributed to N Morimoto.

At least 73 records · Page 4Linked to original sources
PubMed1991

[Study on choroidal blood flow at dark and light adaptation. II. Choroidal blood flow at light adaptation].

The effect of light adaptation on choroidal blood flow (CBF) was studied in albino rabbits. CBF was measured by the hydrogen clearance method. There was no significant change of CBF at light adaptation, in which CBF had been expected to decrease due to decreased oxygen demand of the outer retina. The results showed that light adaptation did not influence CBF, in spite of the close relationship between the outer retina and choroidal circulation. Because of the rich blood flow, the choroidal circulation may not need a reaction mechanism for functional changes of the outer retina.

Adaptation, Ocular↗
PubMed1990

Retinal vasculitis as a complication of rheumatoid arthritis.

Two middle-aged women developed retinal vasculitis in the moderately active phase of classical rheumatoid arthritis. Fluorescein angiography disclosed diffuse leakage from the retinal capillaries and cystoid macular edema, which subsided in response to oral steroid. They did not show any clinical signs of vasculitis in other parts of the body. Retinal vasculitis should be included in the list of complications observed in rheumatoid arthritis.

Adrenal Cortex Hormones↗
PubMed1990

Fine structure of tyrosine hydroxylase-like immunoreactive neurons and terminals of the hypothalamic arcuate nucleus and median eminence in young and aged rats.

Tyrosine hydroxylase (TH)-like immunoreactive neurons, fibers, and terminals in the hypothalamic arcuate nucleus and median eminence of young (8 weeks old) and aged (24 months old) rats were investigated at light and electron microscopic levels by means of the peroxidase anti-peroxidase (PAP) method. In the arcuate nucleus, TH-like immunoreactive neuronal perikarya of young animals contained well-developed cell organelles such as rough-surfaced endoplasmic reticulum (rER), mitochondria, Golgi apparatus, polysomes, as well as dense granules. Immunoreactive neuronal elements appeared as both presynaptic and postsynaptic elements. In aged rats, TH-like immunoreactive neuronal perikarya as well as non-immunoreactive ones were found to include many lysosomes or inclusions, and many neuronal elements with degradative changes were observed. In the external layer of the median eminence of young animals, many immunoreactive nerve endings were found around the pericapillary spaces of portal vessels and often terminated adjacent to the basement membranes of the pericapillary spaces. Immunoreactive fibers having close contact with non-immunoreactive fibers were also observed. In aged animals, degradative changes such as axons with swollen and watery appearance and myelin figures were observed in the neuronal elements, although many immunoreactive nerve endings were seen around the pericapillary spaces.

Animals↗
PubMed1990

[Recognition of orthodontic patients and their parents about the orthodontic treatment and results--a questionnaire method].

This study was undertaken to investigate the desire and consciousness of orthodontic patients and their parents on the content and effect of orthodontic treatment. The subjects were 362 post-treatment patients and 353 of their parents, who answered the questionnaires. The following conclusions were obtained: 1. 49% of the patients and 16% of the parents of the patients thought of giving up the treatment while the patients were under the orthodontic treatment. The main reasons were the discomfort of orthodontic appliances, long treatment period, and the absence from school. 2. 55% of the patients felt uneasy about a change of the occlusion for the worse after removal of the orthodontic appliances, and 60-70% of the patients and parents had forgotten the necessity of the retainer after active orthodontic treatment. 3. Both patients and parents hoped to finish the orthodontic treatment by the end of junior high school. 4. About 50% of the parents preferred the university hospital and about 45% preferred a private dental office which was convenient for attending as an outpatient. 5. About 70% of the patients and parents were satisfied with occlusion after orthodontic treatment, and 33% of the patients and 61% of the parents were satisfied with the orofacial appearance after treatment. 6. About 9% of the patients hoped to keep secret their history of orthodontic treatment. 7. About 35% of the patients and 60% of the parents would recommend people with malocclusions to receive orthodontic treatment. About 80% of the patients would make their own children receive the orthodontic treatment if needed. 8. About 90-95% of the patients and parents were pleased with orthodontic treatment.

Humans↗
PubMed1990

[A case of crystalline retinopathy with crystalline deposits in the cornea].

We experienced a case of crystalline retinopathy with crystalline deposits in the cornea and the limbal conjunctiva, and performed histological studies of the cornea, conjunctiva and peripheral blood cells. The patient was a 45-year-old man who had no abnormality in hematological studies and urinalysis. In the fundus diffuse atrophy of the retinal pigment epithelium and patchy foci of choroidal atrophy were observed, and sparkling yellowish-white spots were scattered, chiefly in the posterior region. Fluorescein angiography showed neither hyperfluorescence nor hypofluorescence at these spots. In the marginal cornea and the limbal conjunctiva crystalline deposits were observed. Histological findings by light microscopy and electron microscopy revealed sparse degeneration of epithelial cells in both the cornea and the limbal conjunctiva. But no substances corresponding to crystalline deposits could be identified. Inclusion bodies were detected including clefts in circulating lymphocytes and eosinophils. The inclusion bodies suggest that crystalline retinopathy results from a systemic metabolic abnormality.

Conjunctiva↗
PubMed1990

[Three cases of alveolar soft part sarcoma and MRI findings].

Three cases of alveolar soft part sarcoma were reported with characteristic MRI findings. On MRI findings, slightly higher signal intensity than muscles on T1 weighted image and remarkably high signal intensity on T2 weighted image were thought to be characteristic of alveolar soft part sarcoma.

Adult↗
PubMed1990

[A clinical study of intraarterial infusion therapy with CDDP for primary osteosarcoma].

Eleven patients with primary osteosarcoma were treated by intra-arterial infusion of cisplatin. Cisplatin (50-100 mg/m2) was infused slowly into tumor feeding artery or proximal-to-the-lesion artery. An antidote, sodium thiosulfate, was also administered intravenously in 10 cases and angiotensin II was simultaneously used in 2 cases. Symptoms of heat sensation and local pain were decreased or disappeared in almost all cases and histopathologic changes were observed in 7 cases. No viable tumor cells were seen in 3 cases.

Adolescent↗
PubMed1989

[Study on choroidal blood flow at dark and light adaptation. 1. Choroidal blood flow at dark adaptation].

The effect of dark adaptation on choroidal blood flow (CBF) was studied in albino rabbits. CBF was measured by the hydrogen clearance method. There was no significant change of CBF at dark adaptation in both short term dark adaptation, in which CBF had been expected to increase due to increased oxygen demand of the outer retina, and long term dark adaptation, in which CBF had been expected to decrease due to depressed function of visual cells and retinal pigment epithelium. The results show that dark adaptation does not influence CBF, in spite of the close relationship between the outer retina and the choroidal circulation. The large blood flow of the choroidal circulation may not need the reaction mechanism for the functional changes of the outer retina.

Animals↗
PubMed1988

Age-related changes in growth hormone releasing factor and somatostatin in the rat hypothalamus.

Distribution and staining intensities of growth hormone releasing factor (GR (GRF) and somatostatin (SRIF) were examined in young (3 months of age) and old (24 months of age) male rats of Sprague-Dawley strain, using the PAP immunocytochemical procedure. Some animals of each age group were intraventricularly injected with colchicine to demonstrate immunoreactive neuronal perikarya. GRF-immunoreactive intensities of old rats were markedly reduced in the median eminence as compared with those of young rats. No remarkable difference could be detected between SRIF immunoreactivities in the young and old animals, since intensive SRIF immunoreactivities were found in the external layer of the median eminence of both groups of animals. Between two age groups injected with colchicine, we also found no difference in the distribution and staining intensities of immunoreactive perikarya of GRF and SRIF in the hypothalamus and also detected no significant difference in total neuron numbers of each peptide. These findings suggest that the synthesis and/or release of GRF in GRF-containing neurons are decreased, though GRF-containing neurons themselves remain alive and have the capacity to synthesize GRF.

Aging↗
PubMed1987

Distribution of neurotensin-like immunoreactivity in the diencephalon of the Japanese monkey (Macaca fuscata).

The distribution of neurotensin-like immunoreactive (NT-LI) neurons was examined in the thalamus and hypothalamus of the Japanese monkey (Macaca fuscata) by using the peroxidase-antiperoxidase immunocytochemistry technique. In the thalamus, NT-LI neuronal perikarya were distributed mainly in the midline nuclear group and the dorsomedial nucleus, and partially in the intralaminar nucleus: Immunoreactive fibers were mainly distributed in the midline nucleus, particularly in the nucleus rhomboidalis. Numerous immunoreactive fibers were also detected in the regions that contain the pathways to extrathalamic areas such as the stratum zonale and inferior thalamic peduncle. In the hypothalamus, many immunoreactive neuronal perikarya were distributed in the lateral hypothalamic area and in the arcuate nucleus. Immunoreactive fibers were disseminated throughout the hypothalamus, but they were dense in the preoptic area and sparse in the ventromedial nucleus. An accumulation of dense immunoreactive endings was also observed in the external layer of the median eminence. NT-LI fibers in the external layer of the median eminence were considered to represent nerve endings near portal vessels. Functional roles of neurotensin in the thalamus and hypothalamus are discussed from the anatomical point of view.

Animals↗
PubMed1987

A resuscitation puzzle: acute acquired methemoglobinemia.

We treated a 32-day-old baby suffering acute acquired methemoglobinemia induced by topical application of aniline cocaine. Although acute acquired methemoglobinemia with severe cyanosis and distress is potentially fatal, this rare syndrome is easily curable if it is correctly diagnosed.

Anesthetics, Local↗
PubMed1986

Light and electron microscopic immunocytochemistry of GRF-like immunoreactive neurons and terminals in the rat hypothalamic arcuate nucleus and median eminence.

Growth hormone-releasing factor (GRF) synthesizing neuronal perikarya and terminals were investigated by light and electron microscopic immunocytochemistry using rat hypothalamus. Immunoreactive neuronal perikarya were located mainly in the ventrolateral part of the arcuate nucleus. They contained well developed cell organella such as mitochondria and rough surfaced endoplasmic reticulum with some expansion. They also contained immunoreactive dense granules (80-120 nm in diameter). On the surface of the immunoreactive neuronal perikarya were frequently found non-immunoreactive axo-somatic synapses. Therefore, the GRF-like immunoreactive neurons were assumed to receive neuronal inputs from other neurons on their neuronal soma. In the external layer of the median eminence large numbers of immunoreactive terminals were distributed particularly around the capillaries of the portal vessel. Electron microscopic immunocytochemistry revealed large numbers of immunoreactive terminals containing immunoreactive dense granules, synaptic vesicles and mitochondria in the vicinity of the basement membrane of the pericapillary space of the portal vessel. Therefore, we concluded that GRF-like immunoreactive substances are released into the portal capillaries from the nerve terminals, which originate from the neuronal perikarya in the ventrolateral part of the arcuate nucleus, and act on growth hormone release in the anterior pituitary. We also suggest that GRF-like immunoreactive neurons have abundant terminal arborization in the external layer of the median eminence.

Animals↗
PubMed1985

Regulatory role of cysteine dioxygenase in cerebral biosynthesis of taurine. Analysis using cerebellum from 3-acetylpyridine-treated rat.

The effect of 3-acetylpyridine (3-AP) administration on the biosynthesis of taurine in the rat brain has been studied. Treatment with 3-AP induced a significant decrease in the cerebellar contents of taurine and its metabolic precursors, cysteine sulfinic acid (CSA) and cysteic acid (CA), as well as a selective degeneration of climbing fibers in the molecular layer of the cerebellum. It was found that the activity of cerebral cysteine dioxygenase, the enzyme catalyzing the formation of CSA from cysteine, consisted of two systems with low and high Km values. The 3-AP-induced attenuation of cysteine dioxygenase activity with a low Km value was noted only in the cerebellum, while that with a high Km value was detected not only in the cerebellum but also in other brain areas such as the medulla oblongata, striatum and cerebral cortex. In contrast, no alteration in the activity of cysteine sulfinic acid decarboxylase (CSD) was observed in any brain areas examined following the administration of 3-AP. Furthermore, it was found that essentially no cystamine as well as a very low activity of cysteamine dioxygenase is present in the brain. The present results suggest that taurine in the brain is synthesized from cysteine, mainly by the CSA and CA pathways, and the observed decline of cerebellar taurine in 3-AP-treated rats may be due to an attenuation of the biosynthesis, possibly at the step of cysteine dioxygenase. A possible regulatory role of cysteine dioxygenase with a low Km value in the biosynthesis of cerebral taurine is also suggested.

Animals↗
PubMed1985

Light and electron microscopic immunocytochemistry of beta-endorphin/beta-LPH-like immunoreactive neurons in the arcuate nucleus and surrounding areas of the rat hypothalamus.

beta-Endorphin/beta-LPH-like immunoreactive neurons in the hypothalamic arcuate nucleus and its surrounding areas were visualized by light and electron microscopic immunocytochemistry. Immunoreactive processes were found in the vicinity of the pia mater, in the lateral part of the external layer of the median eminence and near the lateral wall of the third ventricle. Neuronal perikarya contained immunoreactive dense granules as well as developed cell organellae. They received neuronal inputs from other neurons through axoplasmic and axodendritic synapses. Immunoreactive neuronal processes containing dense granules and mitochondria were found as preterminal elements on non-immunoreactive neuronal soma and dendrites. Immunoreactive processes also make intimate contact with capillaries in the arcuate nucleus near the median eminence.

Animals↗
PubMed1985

Plasma fibrinolysis after intraduodenal administration of urokinase in rats.

Plasma fibrinolysis in rats rose above the level of physiological fluctuations in a curve with two peaks at 1 and 6 h, following intraduodenal administration of high-molecular-weight urokinase (HMW-UK; MW 53,000; 124,000 IU/mg protein). Activation of plasma fibrinolysis was also confirmed with insolubilized enzyme (glass-coupled UK), but lacked the first activity peak. Plasma fibrinolytic enzyme isolated by affinity chromatography revealed strong fibrinolytic (1,120 IU/dl), pyro-Glu-Gly-Arg-pNA amidolytic (3,200 nmol/dl) and Glu-plasminogen activating (24.5 IU/dl) activities. Using specific UK antibody, it appeared that the first peak originated from the administered UK, while the second one derived from endogenous plasminogen activator. Dose response of UK was not observed, and the maximal effect was at about 5,000 IU/kg body weight.

Animals↗
PubMed1984

Influence of ascending noradrenergic fibers on the neurotensin-like immunoreactive perikarya and evidence of direct projection of ascending neurotensin-like immunoreactive fibers in the rat central nucleus of the amygdala.

The influence of ascending noradrenergic neuronal input on the neurotensin (NT)-like immunoreactive neuronal perikarya located in the dorsal part of the central nucleus of the amygdala (CNA) was examined using fluorescence histochemistry and peroxidase-antiperoxidase (PAP) immunocytochemistry. Unilateral hemitransection of the ascending noradrenergic pathway by injection of 6-hydroxydopamine into the caudal mesencephalon just rostral to the locus coeruleus caused a marked depletion of immunoreactivity in NT-like immunoreactive neuronal perikarya in the CNA. Ascending noradrenergic neuronal input, therefore, is considered to facilitate production of NT-like immunoreactive substances in neuronal perikarya and to influence on the functional role of the amygdaloid complex. In addition, we obtained evidence of unilateral direct ascending projections of NT-like immunoreactive neurons into the CNA since the disappearance of NT-like immunoreactive processes occurred mainly in the ventral part of the CNA after surgical hemitransection of the ascending neuronal pathway that interrupts the ascending NT-like immunoreactive pathway arising from the neurons in the brain stem.

Amygdala↗
PubMed1984

Light and electron microscopic immunocytochemistry of neurotensin-like immunoreactive neurons in the rat hypothalamus.

Neurotensin-like immunoreactive neuronal perikarya, fibers and terminals in the rat hypothalamus, particularly in the arcuate nucleus, the paraventricular nucleus and the median eminence, were investigated by light and electron microscopic immunocytochemistry. The main distributional areas of immunoreactive neuronal perikarya were found to be the arcuate nucleus, the periventricular nucleus and the paraventricular nucleus by light microscopic immunocytochemistry. Immunoreactive neuronal perikarya showed a characteristic distributional pattern in the arcuate nucleus. In the paraventricular nucleus they were distributed in both the magnocellular and parvocellular portions. A large number of immunoreactive terminals were observed throughout the external layer of the median eminence, particularly its lateral portion. A moderate number of immunoreactive terminals were also observed in the internal layer of the median eminence. By electron microscopic immunocytochemistry immunoreactive neuronal perikarya both in the arcuate and paraventricular nuclei showed generally well-developed cell organelles such as mitochondria, r-ER, and Golgi complex. In addition, immunoreactive dense granules were dispersed throughout the perikarya. A large number of immunoreactive terminals containing immunoreactive dense granules, clear vesicles and mitochondria were observed in the vicinity of pericapillary spaces of the external layer of the median eminence. This observation strongly suggests that neurotensin-like immunoreactive substance is released into the portal capillaries.

Animals↗