Biomedical subjects
Publications and source records attributed to N N Musienko.
Steroid glicoside which structure was elucidated by using mass-spectroscopic and NMR techniques, has been isolated using column chromatography on reverse-phase silicagel (C-18) from underground parts of Allium nutans. This compounds for the first time was isolated from the plants of genus Allium.
Kinetics of pyruvate transport through the envelope chloroplast vesicles has been studied. For wheat cultivar Bezostaya-1 Km is 26 mM, Vmax--33 Mmol/l h per 1 mg of protein, Hill's coefficient--0.56 and the coefficient of temperature inactivation at 40 degrees C is 0.045 min-1. 7 mM Mg or Ca ions for optimal activity of the system are needed. Chloroplast treatment with trypsin increases the pyruvate transport intensity. The simple analytical method is suggested for pyruvate determination at the concentration of 10-150 micrograms/ml.