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N S Penneys

Publications and source records attributed to N S Penneys.

At least 145 records · Page 8Linked to original sources
PubMed1984

A survey of cutaneous neural lesions for the presence of myelin basic protein. An immunohistochemical study.

The presence of myelin basic protein (MBP) within a skin neoplasm would support its derivation from Schwann's cells, since this substance is routinely present within Schwann's cells in the peripheral nervous system. Using a monoclonal antibody prepared against MBP and an unlabeled antibody peroxidase-antiperoxidase assay, we surveyed a variety of skin lesions suspected of being derived from Schwann's cells to determine whether MBP was present. Myelin basic protein was detected within the cytoplasm of cells composing benign solitary schwannoma (neurilemmoma) and neurofibroma, confirming the association of these lesions with proliferation of Schwann's cells. Myelin basic protein was not found in a variety of intradermal and compound nevus cell nevi nor in malignant melanoma. This negative finding supports electron microscopic evidence suggesting that nevus cells have no relationship to Schwann's cells even though some nevus cell arrangements suggest Schwann's cell derivation under the light microscope.

Animals↗
PubMed1984

Prekeratin immunohistochemistry in the diagnosis of undifferentiated carcinoma of the nasopharyngeal type.

We investigated the value of prekeratin immunostaining in establishing the diagnosis of undifferentiated carcinoma of the nasopharyngeal type (lymphoepithelioma). As in squamous cell carcinoma of the nasopharynx, all seven lymphoepithelial carcinomas stained for prekeratin whereas other look-alike but histogenetically different neoplasms (malignant lymphoma, esthesioneuroblastoma, and small round cell sarcomas) did not stain. In addition to confirming the squamous epithelial nature of lymphoepithelioma, our findings indicated that immunoperoxidase staining for prekeratin is a useful diagnostic tool in the differential diagnosis of nasopharyngeal carcinomas in general and lymphoepithelioma in particular.

Animals↗
PubMed1983

Subpicogram analysis of sweat proteins using two-dimensional polyacrylamide gel electrophoresis.

Sweat collected from six normal volunteers was analyzed to determine if reproducible protein patterns could be obtained using two-dimensional polyacrylamide gel electrophoresis of 125I-labeled sweat proteins. This method has the capability of easily detecting picogram quantities of protein. Once the methods of collection of the sweat had been standardized, reproducible patterns were obtained from these volunteers. Over 100 discrete spots were revealed by a combination of fluorography and rare earth screen radioautography of dried two-dimensional gels. This method will allow analysis of sweat for qualitative and quantitative variations in protein content in pathologic conditions such as cystic fibrosis, renal failure, and diabetes.

Electrophoresis, Polyacrylamide Gel↗
PubMed1983

Fibrous papule: an immunohistochemical study with an antibody to S-100 protein.

We studied twenty biopsies taken from fibrous papules which were located in the central part of the face. The lesions, which were characteristic histologically, did not contain S-100 protein within the stellate cells in the papillary dermis, nor was this substance found in mesenchymal cells with some features of nevus cells. In control studies, S-100 protein was found using an unlabeled antibody peroxidase-antiperoxidase technic within nevus cells composing junctional intradermal, and compound nevi, Spitz nevi, and halo nevi. It therefore seems unlikely that fibrous papule represents a form of degenerated nevus as some investigators have proposed.

Biopsy↗
PubMed1983

Immunoreactive prekeratin in Brenner tumors of the ovary.

In an attempt to further characterize benign, proliferating, and malignant Brenner tumors, immunoperoxidase staining for prekeratin was undertaken in a group of these neoplasms as well as in a variety of histologically different tumors of the ovary. All Brenner tumors reacted positively for prekeratin, whereas none of the other epithelial or sex cord-stromal tumors of the ovary stained. On the basis of these findings, we conclude that prekeratin might be a useful marker for differentiating malignant Brenner tumors from histologically similar, poorly differentiated ovarian neoplasms.

Brenner Tumor↗
PubMed1983

Granular cell tumors of the skin contain myelin basic protein.

Myelin basic protein, a substance found in neural structures, has been demonstrated in cutaneous granular cell tumors using a monoclonal antibody generated against myelin basic protein and an immunoperoxidase method. The substance was not found in fibrohistiocytic skin lesions. The presence of myelin basic protein in granular cell lesions of the skin supports the concept that this lesion is related closely to nerve structures.

Animals↗
PubMed1983

Prekeratin in spindle cell tumors of the skin.

A variety of benign and malignant skin lesions was stained for the presence of prekeratin using an unlabeled antibody peroxidase-antiperoxidase method and an antibody raised against human prekeratin protein. No prekeratin could be detected in benign or malignant lesions derived from melanocytes, and prekeratin could not be found in atypical fibroxanthoma, Kaposi's sarcoma, angiosarcoma, dermatofibrosarcoma protruberans, and leiomyosarcoma. Paget's cells did not contain prekeratin. Varying staining intensities were observed in keratinocytic atypical hyperplasias. Of particular interest is the observation that many spindle cell lesions that were difficult to identify with 100% assurance from routine stains could be positively identified as squamous cell carcinoma after prekeratin was found in the atypical cells.

Animals↗
PubMed1982

Paget's disease of the skin. A unifying concept of histogenesis.

Utilizing the peroxidase antiperoxidase technique we explored the presence and the distribution of carcinoembryonic antigen (CEA) in seven cases of mammary and sixteen of extramammary Paget's disease of skin. In every case positive immunostaining was observed in all the Paget's cells and the underlying tumor (where one was present), whereas intervening keratinocytes and melanocytes did not stain. CEA was also present in the cells and secretions of normal eccrine and apocrine glands. Our observations confirm that Paget's cells are of glandular origin.

Adenocarcinoma↗
PubMed1982

Carcinoembryonic antigen in sweat-gland carcinomas.

Using standard immunoperoxidase techniques, four cases of malignant sweat-gland carcinoma were examined for the presence of carcinoembryonic antigen (CEA). This substance was universally present in these lesions in spite of their wide spectrum of histologic features. CEA was mainly localized within the cytoplasm of tumor cells and in the lumina of neoplastic glands. The findings indicate that the presence of CEA in malignant skin adnexal tumors could help to identify and categorize these lesions as derived from eccrine or apocrine adnexal epithelium.

Adenocarcinoma↗
PubMed1982

Sinus histiocytosis with massive lymphadenopathy: a case with unusual skin involvement and a therapeutic response to vinblastine-loaded platelets.

A patient with sinus histiocytosis with massive lymphadenopathy had unusual skin lesions and progressive internal involvement. Both aspects responded dramatically to the administration of vinblastine-loaded platelets. Vinblastine-loaded, idiopathic thrombocytopenic purpura antibody-coated platelets are a rationale therapy for a disease characterized by the presence of actively phagocytosing histiocytes.

Antibodies↗
PubMed1982

Kaposi's sarcoma: absence of cytomegalovirus antigens.

Using an immunohistochemical method, we could not detect cytomegalovirus antigens in the tissue of Kaposi's sarcoma but could in the cells of control sections from tissues known to be infected by that virus. Our results are consistent with the hypothesis that high antibody titers to cytomegalovirus found in patients with Kaposi's sarcoma are not due to infection of neoplastic cells by the virus, as a secondary event; our data supports the conclusions of others that exposure to cytomegalovirus may be a primary event in the pathogenesis of Kaposi's sarcoma.

Antigens, Viral↗
PubMed1982

Inhibition of cell-mediated immune reactions by an aqueous extract of normal human skin.

An aqueous extract of normal human skin has been shown to contain an inhibitor of certain cell mediated immune reactions. In this report, the effect of the inhibitor on cell membrane markers and antibody dependent cellular cytotoxicity was determined. Significant diminution of E rosette formation was demonstrated using as little as 0.6 microgram of the skin fraction (p less than .02). Fc receptors for both IgG and IgM were reduced by 46-96% of controls in the presence of the skin inhibitor. On the other hand, no effect on the detection of the complement receptor or surface immunoglobulin was observed, indicating some specificity of binding. In addition, the antibody dependent cell-mediated cytotoxic reaction was inhibited on the skin extract. It was shown that the inhibitor interacted with the lymphocytes, not the antibody or target cells. No effect was detectable when the skin fraction was added after the interactions of effector cells, antibody, and target cells had occurred. This was in contrast to PHA-induced cytotoxicity which could be inhibited following the preincubation of the lymphocytes with the mitogen. Thus there appears to be 2 mechanisms by which the skin fraction interferes with cellular responses: inhibition of antibody binding to Fc receptors, and interference with a step in cellular activation following mitogen stimulation. Analysis of the extract showed the inhibitor was inactivated by trypsin, and did not contain sialic acid, 5'-nucleotidase of beta-N-acetylglucosaminidase, and thus was not associated with membrane or lysosomal enzymes.

Animals↗
PubMed1982

Inhibition of arachidonic acid oxidation in vitro by vehicle components.

In this work, we observed that several vehicle components which are complex lipids inhibited the oxidation of arachidonic acid in the oxygen monitor assay. Inhibition of oxygen consumption in this assay, a relatively non-specific finding, may reflect inhibition of lipoxygenase and/or prostaglandin synthetase activity or possible sequestration of arachidonic acid. Theoretically, similar effects could occur in vivo. Consideration should be given to the formulation of vehicles which maximize the anti-inflammatory effects of vehicle components.

Animals↗
PubMed1982

Carcinoembryonic antigen in benign sweat gland tumors.

Carcinoembryonic antigen was demonstrated in sweat gland adenomas by immunostaining using standard immunoperoxidase techniques. Carcinoembryonic antigen could not be found in adnexal tumors derived from the pilar and sebaceous apparatus. The demonstration of this specific antigen in certain sweat gland tumors may be helpful in the recognition and classification of these kinds of lesions.

Adenoma↗
PubMed1981

Carcinoembryonic antigen present in human eccrine sweat.

Carcinoembryonic antigen is detectable by standard unlabeled peroxidase-antiperoxidase technics in eccrine and apocrine secretory and ductal cells. Carcinoembryonic antigen is also present in the cuticle which lines the duct. In eccrine sweat, carcinoembryonic antigen activities were at least thirty times that in serum, using a radioimmunoassay. Carcinoembryonic antigen may form part of the Schiff-positive cuticle and function in the normal homeostasis of eccrine and apocrine glands.

Apocrine Glands↗