Biomedical subjects
Publications and source records attributed to N S Penneys.
We review a spectrum of unusual infectious, nutritional, and malignant processes that have been observed in the skin of patients with acquired immunodeficiency syndrome.
An immunohistochemical method for the demonstration of S-100 protein was used to identify melanocytes in fourteen cases of malignant melanoma subsequently excised after initial punch biopsy. Although S-100 protein-containing cutaneous nerves and dermal dendritic cells could be identified within the area of dermal repair, there was no evidence of physical movement of atypical melanocytes or nevus cells downward into the scarified area. This study cannot confirm the concept that punch biopsy of melanoma physically seeds atypical cells into the dermis.
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We examined 4 cases of sclerosing epithelial hamartoma for the presence of carcinoembryonic antigen (CEA) by the unlabelled antibody peroxidase-antiperoxidase method but could not detect CEA in these lesions. Because syringomas contain CEA, the detection of this antigen is a useful marker for differentiating sclerosing epithelial hamartomas from syringomas.
Fifteen halo nevi were stained for the presence of S100 protein by an unlabelled antibody peroxidase-antiperoxidase method. S100 protein was clearly identifiable within nevus cell nests in the inflammatory infiltrate. The presence of this substance helped to identify nevus cells in dense inflammatory infiltrates and confirm the histologic diagnosis of halo nevus.
A 78-year-old woman with a history of follicular carcinoma of the thyroid developed a painless nodule on her face. A skin biopsy showed a dermal tumor composed of cells with granular, eosinophilic cytoplasm. Immunoperoxidase staining demonstrated thyroglobulin in the tumor cells, confirming the diagnosis of metastatic thyroid carcinoma.
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A 29-year-old Haitian man with the acquired immune deficiency syndrome (AIDS) developed disseminated cryptococcosis with an unusual cutaneous presentation. He had numerous hypopigmented papules over his face that clinically resembled molluscum contagiosum. To our knowledge, cutaneous cryptococcosis resembling molluscum contagiosum has not been previously reported. The patient had a T-cell defect consistent with AIDS and belonged to a high-risk group. To our knowledge, this is the second report of cutaneous cryptococcosis in a patient with AIDS.
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We surveyed a variety of papillomatous lesions, trichilemmoma, and bowenoid papulosis for the presence of papillomavirus common antigen using an unlabeled antibody peroxidase-antiperoxidase technique and an antibody against papillomavirus common antigen. Papillomavirus common antigen was detected in four of six verruca vulgaris lesions overlying the nuclei of koilocytotic cells. The antigen was observed much less often in the granular zone of verrucous lesions that did not contain koilocytotic cells. Thirteen of 18 lesions of bowenoid papulosis had positive immunostaining overlying occasional nuclei in the granular zone but the antigen was not detected in the granular zone of squamous cell carcinoma in situ or of trichilemmoma. These data support the hypothesis that some lesions of bowenoid papulosis represent a form of cutaneous infection by papillomavirus.
This review presents an abbreviated discussion of a technic the application of which is having profound effects on approaches to pathologic diagnosis, classification of tumors, analysis of cell infiltrates in the skin, and a variety of research problems. Its use will continue to increase as more physicians, pathologists, and researchers become acquainted with its potential.
In five patients with symptomatic lichen sclerosus, significant clinical improvement was obtained following the administration of potassium para-aminobenzoate. Improvement was characterized by a marked decrease or absence of symptoms and a flattening of skin lesions.
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The abnormal gene product in cystic fibrosis has not been identified. We report that two-dimensional polyacrylamide gel electrophoresis of 125I-labeled sweat proteins coupled with fluorography and rare earth screen radioautography reveals an absence of an acidic protein of molecular weight 60,000 in sweat collected from 9 patients with cystic fibrosis. The protein spot was present in sweat from heterozygotes and from controls.
Developing fetal eccrine glands were examined for the presence of carcinoembryonic antigen (CEA) using an unlabeled antibody peroxidase-antiperoxidase technique and rabbit antibody to human CEA. The earliest sign of commitment to the formation of an eccrine gland was the presence of CEA within a single keratinocyte in fetal epidermis. As the eccrine unit evolved, we found CEA in both acrosyringeal cells and the ductal cells. Although the function of CEA in eccrine gland remains undefined, this substance may play an integral role in the earliest development of the gland as well as in its normal function in the adult.
Using an unlabelled antibody peroxidase-antiperoxidase method, we have demonstrated that certain tissue antigens can be identified in tissue sections previously stained with hematoxylin and eosin. This technique is useful in identifying antigens in tissues where insufficient unstained material is available.
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