Biomedical subjects

N V Repin

Publications and source records attributed to N V Repin.

3 recordsLinked to original sources

PubMed1994

Quantitative biological electron probe microanalysis with a wavelength dispersive spectrometer.

This paper describes the details of quantitative electron probe microanalysis (EPMA) performed with a wavelength dispersive spectrometer (WDS). EPMA was carried out on the giant neuron of a fresh frozen ganglion from the snail Lymnaea stagnalis. The freeze-dried cryosections were compared with sections of freeze-dried, embedded tissue. It was found, that in the ganglion there are two kinds of neurons with a different chlorine concentration of 11 mmole/liter and 32 mmole/liter. Isolated neurons in culture were shown to differ in elemental composition from those in the ganglion tissue.

Animals↗

PubMed1990

[The ultrastructural and dynamic characteristics of erythrocyte membranes. The effect of the physiological status and temperature].

Erythrocyte membranes of ground squirrels taken from hibernating, awakening and active animals with the body temperatures of 4, 30 and 36 degrees C, respectively, have been shown by freeze-fracture technique to differ in morphology of the PF surface, density and distribution patterns of intramembrane particles. The ultrastructural changes in membrane caused by temperature were confirmed by the data on alterations of dynamics parameters of the lipid bilayer and spectral characteristics of membrane proteins.

Animals↗

PubMedDate not supplied

[Structural and functional characterization of cytochrome oxidase encapsulated in liposomes in sodium chloride solutions].

The effect of NaCl concentration (0-2 M) on the absorption spectrum and enzymic activity of cytochrome oxidase built in lecithin liposome has been studied at 24 and 8 degrees C. It is shown that at 0,8 M NaCl concentration a conformational transition occurs that leads to the enzyme inactivation. The relationship between protein conformation and liposome state has been established.

Animals↗