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PubMed · 7638478

Quantitative biological electron probe microanalysis with a wavelength dispersive spectrometer.

A Pogorelov·V Pogorelova·N V Repin·I Mizin
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Abstract

This paper describes the details of quantitative electron probe microanalysis (EPMA) performed with a wavelength dispersive spectrometer (WDS). EPMA was carried out on the giant neuron of a fresh frozen ganglion from the snail Lymnaea stagnalis. The freeze-dried cryosections were compared with sections of freeze-dried, embedded tissue. It was found, that in the ganglion there are two kinds of neurons with a different chlorine concentration of 11 mmole/liter and 32 mmole/liter. Isolated neurons in culture were shown to differ in elemental composition from those in the ganglion tissue.

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AnimalsElectron Probe MicroanalysisFreeze DryingFreeze FracturingGanglia, InvertebrateLymnaeaMathematicsMicroscopy, Electron, Scanning TransmissionNeuronsSensitivity and SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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A Pogorelov, V Pogorelova, N V Repin, I Mizin. 1994. Quantitative biological electron probe microanalysis with a wavelength dispersive spectrometer.. https://pubmed.ncbi.nlm.nih.gov/7638478/

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