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Biomedical subjects

N W Preston

Publications and source records attributed to N W Preston.

At least 55 records · Page 3Linked to original sources
PubMed1984

Association between Bordetella pertussis agglutinogen 2 and fimbriae.

Fimbriae have been demonstrated on strains of Bordetella pertussis that possess agglutinogen 2 (types 1, 2, 3 and 1, 2), but not on those that lack it (types 1,3 and 1). This correlation between fimbriation and the presence of agglutinogen 2 has been found with fresh isolates from children and with laboratory strains that are virulent for mice. If fimbriae enhance the attachment of bacteria to mucosal cells, these findings offer an explanation for the predominance of serotypes 1,2,3 and 1,2 in non-vaccinated communities. The findings also suggest that agglutinogen 3 is not a fimbrial antigen, and because this is an essential component of fully effective whole-cell vaccine, a subcellular vaccine prepared from fimbriae alone may be inadequate.

Agglutination Tests↗
PubMed1982

A reappraisal of serotype factors 4, 5 and 6 of Bordetella pertussis.

Twelve strains of Bordetella pertussis, of various serotypes, were tested by agglutination with serial dilutions of six antisera which were thought by their donor to be monospecific for six distinct agglutinogens. The first three sera were shown, by agglutination of the twelve donated strains and of standard pertussis serotypes, to be monospecific for the three well-recognized major agglutinogens (factor 1, factor 2, factor 3). The 'factor 4' serum gave weak agglutination, but only of strains that possessed factor 2, though not all of such strains. This suggested that it may be a distinct minor component of factor 2. The 'factor 5' serum was very weak, and gave agglutination only with a few of the factor 2 strains. The titres of the 'factor 6' serum were very closely parallel to those of the factor 3 serum, and it may be inferred that these 'factors' probably constitute a single antigenic entity. Seven of the twelve donated strains were shown to be mixtures of two or more serotypes, offering an explanation for discrepancies in serological tests involving the use of these strains on different occasions. It seems likely that 'factors 5 and 6' do not actually exist; and, since 'factor 4' is only a minor component of factor 2, any protective role that it may have would probably be covered by the presence of factor 2 in vaccine.

Bordetella pertussis↗
PubMed1978

Machakos project studies. Agents affecting health of mother and child in a rural area of Kenya. V. Pertussis sentypes in Kenyan children 1974--1975.

Cultures of Bordetella pertussis were isolated by pernasal swabs from Kenyan children in whom whooping-cough was suspected. Serotyping of 94 of these isolates was undertaken by four laboratories in Europe, and there was very close agreement in their typing results. Each laboratory found that the incidence of type 1, 3 was the lowest of the three types, and that there were approximately equal numbers of types 1, 2, 3 and 1, 2. No new serotype was found. This distribution of serotypes was found in all age-groups; and it is in marked contrast with that currently seen in vaccinated communities, where type 1, 3 predominates. The implications of these findings for vaccination against whooping-cough in East Africa are discussed.

Bordetella pertussis↗
PubMed1976

Protection by pertussis vaccine. Little cause for concern.

It was estimated from a survey of 418 children aged three weeks to twelve years with bacteriologically confirmed whooping-cough that about 95% of vaccinated children are protected by current vaccine. Protection could be increased even further by the greater use of adsorbed rather than plain vaccine and by closer adherence to a vaccination schedule. The lower incidence of infection, resulting from widespread acceptance of this highly effective vaccine, would also greatly reduce the exposure of infants who are too young to be immunished themselves; and eradication ow whooping-cough would be in sight.

Bordetella pertussis↗