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N Yasukawa

Publications and source records attributed to N Yasukawa.

7 recordsLinked to original sources
PubMed2000

Female house mice develop a unique ovarian lesion in colonies that are at maximum population density.

Colonies of house mice reach maximum population density in 120-180 days, irrespective of cage size and initial number of colonizing animals. Reproduction ceases because the females become aggressive and unreceptive to mating. The aggressive behavior is correlated with elevated levels of testosterone (T) and corticosterone (B) (Chapman et al., Phys Behav 64:529-533, 1998). In two of seven strains of mice, females developed ovarian lesions. The occurrence of the lesion in one strain was correlated with the age of the animal and duration of the study. In the second strain, cage size was the determining factor. Lesioned ovaries weighed significantly more than nonlesioned ovaries. The lesion consisted of accumulations of luteal membrane and organelle fragments, and other cellular debris, suggestive of incomplete and prolonged luteolysis. Electron microscopic (EM) analyses revealed the presence of deposits of permanganate-resistant congophilic amyloid fibrils in the intima and smooth muscle cells of luteal thecal arteries. Population females had thymus glands and uteri that weighed significantly less than the same organs from females housed in the breeding colony, whereas the adrenal glands from the population females weighed significantly more. It is proposed that the female aggression is due to high levels of T. It is also proposed that the high levels of B suppress the immune cells involved in normal luteolysis and contribute to the incomplete and prolonged luteolysis.

Aggression↗
PubMed1993

Kinetics of infectivity are dissociated from PrP accumulation in salivary glands of Creutzfeldt-Jakob disease agent-inoculated mice.

The protease-resistant isoform of prion protein (PrP) has been implicated in the pathogenesis and transmission of Creutzfeldt-Jakob disease (CJD), scrapie and other related diseases, but the relationship between the infectious agent and PrP awaits elucidation. In the present study, we have examined levels of infectivity together with accumulation of the protease-resistant form of PrP (PrPCJD) in various tissues of CJD agent-inoculated mice. Accumulation of PrPCJD occurred only in tissues, including brain, salivary gland and spleen, in which infectivity was readily detectable throughout the course of the experiment. The brain showed the highest levels of both infectivity and PrPCJD accumulation, with well correlated kinetics. On the other hand, the high titres of infectivity detected in salivary gland and spleen early after inoculation of the agent were obviously distinguishable from PrPCJD. Furthermore, in the salivary gland, the kinetics of infectivity and the accumulation of PrPCJD reversed; infectivity declined as PrPCJD accumulated in the tissue. Our findings indicate that PrPCJD accumulation is associated with replication of the agent; however, PrPCJD is unlikely to be the agent itself.

Animals↗
PubMed1979

Perinatal naloxone: when does naloxone affect hyperalgesia?

Pregnant mice were treated with naloxone via subcutaneous implants, from about 5 days prior to parturition. At birth entire litters were cross-fostered so that groups of offspring were exposed to naloxone treated mothers; before birth, after birth to weaning, from about 5 days prior to birth to weaning, or not exposed to naloxone. When tested on a hot-plate at 50 days of age, females either prenatally treated or treated pre- and postnatally showed hyperalgesia to heat. For males, this effect was not evident. This sex difference may have been induced by the cross-fostering procedure.

Aging↗
PubMed1978

Estrous cycle regulation in the whitefooted mouse (Peromyscus leucopus) with special reference to vaginal cast formation.

The estrous cycle of the whitefooted mouse (Peromyscus leucopus) was consistent with those described for most small rodents and closely resembled that of the house mouse and the rat in timing. The presence of the male did not accelerate puberty nor induce cycle synchrony, but was critical in establishing cycle regularity. Females isolated from influences of males never attained a pattern of concistent cycle length. Development of the vaginal epithelium during estrus was excessive in both isolated and non-isolated females, resulting in a "cast" of cornified epithelium which could be removed intact during metestrus. Casts removed during the making of vaginal smears measured up to 20 mm in length and still left a sufficient residue of cells which could be detected in all stages of the estrous cycle.

Animals↗
PubMed1975

The quantitative determination of griseofulvin by gas chromatography (ECD-GC).

Serum and organ griseofulvin levels in guinea pigs and serum levels in patients who received a prolonged oral administration of griseofulvin were determined gas-chromatographically. In guinea pigs liver showed the highest concentration of griseofulvin, followed by kidney and serum in the decreasing order. The skin levels were 0.16 approximately 1.51 mug/g with a dose of 30 mg/kg. In humans it was proved that griseofulvin did not cause any accumulation in the serum with a dosage of 500 mg/day.

Administration, Oral↗